Differences in gut microbiota between silkworms (<i>Bombyx mori</i>) reared on fresh mulberry (<i>Morus alba</i>var. multicaulis) leaves or an artificial diet

Dong, Hui-Ling; Zhang, Shengxiang; Chen, Zhuo-Hua; Tao, Hui; Xue, Li; Qiu, Jian-Feng; Cui, Wen-Zhao; Sima, Yang-Hu; Cui, Wei-Zheng; Xu, Shiqiang

doi:10.1039/c8ra04627a

Cited by 48 publications

(50 citation statements)

References 66 publications

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“…Enterobacteriaceae dominated the gut bacteria of S. litura feeding on taro leaves, while Pseudomonadaceae dominated the gut bacteria of S. litura feeding on the artificial diet. Similar results have been found with silkworm in which differences in the gut microbes are seen for those feeding on mulberry leaves or artificial diet with these differences leading changes in nutritional metabolism and immune resistance ( Dong et al, 2018 ). A study in pine processionary moth, Thaumetopoea pityocampa , also revealed that different host plants lead to a different gut bacterial community ( Strano et al, 2018 ).…”

Section: Discussionsupporting

confidence: 81%

Characterization of Spodoptera litura Gut Bacteria and Their Role in Feeding and Growth of the Host

et al. 2020

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Insect gut microbes play important roles in host feeding, digestion, immunity, growth and development. Spodoptera litura is an important agricultural pest distributed of global importance. In the present study, diversity and functions of the gut bacteria in S. litura are investigated based on the approaches of metagenomics and denaturing gradient gel electrophoresis (DGGE). The results showed that the gut bacterial diversity of S. litura reared on taro leaves or an artificial diet, were similar at the phylum level, as both were mainly composed of Proteobacteria, but differed significantly at the order level. Spodoptera litura reared on taro leaves (Sl-tar) had gut biota mainly comprised of Enterobacteriales and Lactobacillales, while those reared on artificial diet (Sl-art) predominantly contained Pseudomonadales and Enterobacteriales, suggesting that gut bacteria composition was closely related to the insect’s diet. We found that feeding and growth of S. litura were significantly reduced when individuals were treated with antibiotics, but could be both restored to a certain extent after reimporting gut bacteria, indicating that gut bacteria are important for feeding, digestion, and utilization of food in S. litura . Metagenomic sequencing of gut microbes revealed that the gut bacteria encode a large number of enzymes involved in digestion, detoxification, and nutrient supply, implying that the gut microbes may be essential for improving the efficiency of food utilization in S. litura .

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Section: Discussionsupporting

confidence: 81%

Characterization of Spodoptera litura Gut Bacteria and Their Role in Feeding and Growth of the Host

et al. 2020

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“…Sequences were grouped into OTUs at a 97% similarity level using UPARSE [ 45 ]. The ribosomal database project (RDP) classifier (Version 16) was used to assign sequences to different taxonomic groups based on the Greengene ribosomal RNA gene database (Version 13.5) [ 46 ]. The sequences were submitted to the Sequence Read Archive in National Center for Biotechnology Information (NCBI, ; accession numbers SRR11206458–SRR11206466).…”

Section: Methodsmentioning

confidence: 99%

Differential Profiles of Gut Microbiota and Metabolites Associated with Host Shift of Plutella xylostella

Yang

Saqib

Chen

et al. 2020

IJMS

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Evolutionary and ecological forces are important factors that shape gut microbial profiles in hosts, which can help insects adapt to different environments through modulating their metabolites. However, little is known about how gut microbes and metabolites are altered when lepidopteran pest species switch hosts. In the present study, using 16S-rDNA sequencing and mass spectrometry-based metabolomics, we analyzed the gut microbiota and metabolites of three populations of Plutella xylostella: one feeding on radish (PxR) and two feeding on peas (PxP; with PxP-1 and PxP-17 being the first and 17th generations after host shift from radish to peas, respectively). We found that the diversity of gut microbes in PxP-17 was significantly lower than those in PxR and PxP-1, which indicates a distinct change in gut microbiota after host shift. Kyoto Encyclopedia of Genes and Genomes analysis revealed that the functions of energy metabolism, signal transduction, and xenobiotics biodegradation and metabolism were increased in PxP-17, suggesting their potential roles in host adaptation. Metabolic profiling showed a significant difference in the abundance of gut metabolites between PxR and PxP-17, and significant correlations of gut bacteria with gut metabolites. These findings shed light on the interaction among plants, herbivores, and symbionts, and advance our understanding of host adaptation associated with gut bacteria and metabolic activities in P. xylostella.

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“…And we found that the butanoic acid content in AF feces was 13.56 times higher than that of the MF feces. The intestinal microbial community diversity of silkworm larvae fed with artificial diet was lower than that of silkworm larvae fed with mulberry leaves [43]. It would be interesting to further explore which gut microbiota causes the intestinal butanoic acid produced after silkworms reared with artificial diets, which further affected the growth and development of the artificial diets-fed silkworm.…”

Section: Certain Metabolites Were More Abundant In the Feces Of The Amentioning

confidence: 99%

Comparative Fecal Metabolomes of Silkworms Being Fed Mulberry Leaf and Artificial Diet

Qin

Wang

Dong

et al. 2020

Insects

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Metabonomics accurately monitors the precise metabolic responses to various dietary patterns. Metabolic profiling allows simultaneous measurement of various fecal metabolites whose concentrations may be affected by food intake. In this study, we analyzed the fecal metabolomes of silkworm (Bombyx mori) larvae reared on fresh mulberry leaves and artificial diets. 57 differentially expressed metabolites were identified by gas chromatography–mass spectrometry. Of these, 39 were up-regulated and 18 were downregulated in the mulberry leaf meal group. Most of the amino acids, carbohydrates and lipids associated with physical development and silk protein biosynthesis were enriched in silkworms reared on mulberry leaves. In contrast, the urea, citric acid, D-pinitol, D-(+)-cellobiose and N-acetyl glucosamine levels were relatively higher in the silkworm feeding on the artificial diets. The findings of this study help clarify the association between diet and metabolic profiling.

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Differences in gut microbiota between silkworms (Bombyx mori) reared on fresh mulberry (Morus albavar. multicaulis) leaves or an artificial diet

Abstract: Artificial diets for silkworms have many potential applications and they are important in sericulture.

Cited by 48 publications

References 66 publications

Characterization of Spodoptera litura Gut Bacteria and Their Role in Feeding and Growth of the Host

Characterization of Spodoptera litura Gut Bacteria and Their Role in Feeding and Growth of the Host

Differential Profiles of Gut Microbiota and Metabolites Associated with Host Shift of Plutella xylostella

Comparative Fecal Metabolomes of Silkworms Being Fed Mulberry Leaf and Artificial Diet

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