Differences in hematologic variables in rats of the same strain but different origin

Abstract

Differences in hematologic RI between Wistar rats obtained from different breeders underscore the need to evaluate untreated control animals for comparison, rather than using historical RI, to detect compound-related effects in preclinical safety studies. Valid RI for control animals are needed and should be verified when using a new supplier to avoid misinterpretation of data. Low reference limits for some variables, such as WBC counts, in rats from one source may preclude their use in studies in which detection of leukopenia is required.

Experimental immunology The effect of vitamin B 6 supplementation of protein deficiency diet on hematological parameters in the blood of rats subjected/non subjected to physical exertion – a pilot study

“…It has been shown, that B 6 lowers cholesterol, prevents atherosclerosis, regulates blood pressure and inhibits the release of histamine and participates in antibodies and red blood cells synthesis [26,27]. The data from hematological analysis of rats fed control diet, presented in this article, were consistent with results obtained for Wistar rats [28]. Generally, we didn't observed significant changes in number of red blood cells (RBC) platelets (PLT) and between rats fed different diets or undergo physical exercise.…”

Progression of anemia and its relationship with renal function, blood pressure, and erythropoietin in rats with chronic kidney disease

“…One methodologic issue to be considered in the interpretation of our studies is the use of published reference intervals to compare our control and disease model data. It is in general not an acceptable practice in clinical pathology, especially in laboratory rats as the origin of the animals can affect such reference intervals . As we are studying a naturally progressive model of disease, no untreated control line is available for reference data; however, this was mediated to some effect by using Lewis animals as controls for the LPK comparative analysis, being the same strain from which the LPK arose, and bred and maintained in the same facility.…”

A novel F8 −/− rat as a translational model of human hemophilia A

“…A global hemostasis test was performed on citrated whole blood by rotational thromboelastometry (Ro-TEM delta; Triolab, M€ unchen, Germany) without activation, with measurement of the clotting time, clot formation time, angle, and maximum clot firmness. Complete blood counts were performed on EDTA blood, with an automated hematology analyser (XT-2000 iV; Sysmex, Norderstadt, Germany), which has been previously utilized in rats [13]. The platelet function test was performed on lithium heparin whole blood, with a Multiplate analyzer (Roche Diagnostics, Rotkreutz, Switzerland), and with activation of the platelets by three different agonists (ADP, arachidonic acid test [ASPI], collagen, and a control [isotonic saline]).…”