Differences in lipid peroxidation of rat liver rough and smooth microsomes

Devasagayam, T.P.A.; Pushpendran, Choliparambil K.; Eapen, Jacob

doi:10.1016/0005-2760(83)90207-2

Cited by 47 publications

(12 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Isolated mitochondria were exposed to oxidative damage induced by tertiary butyl hydroperoxide (TBHP) and ascorbic acid and iron containing system (As-Fe 2+ ) [33]. Briefly, mitochondria corresponding to 500 μg of protein were incubated with 400 μM TBHP and As-Fe 2+ in the presence or absence of different concentrations of Pterostilbene for 60 mins at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Protective effect of Pterostilbene against free radical mediated oxidative damage

Acharya

Ghaskadbi

2013

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundPterostilbene, a methoxylated analog of Resveratrol, is gradually gaining more importance as a therapeutic drug owing to its higher lipophilicity, bioavailability and biological activity than Resveratrol. This study was undertaken to characterize its ability to scavenge free radicals such as superoxide, hydroxyl and hydrogen peroxide and to protect bio-molecules within a cell against oxidative insult.MethodsAnti-oxidant activity of Pterostilbene was evaluated extensively by employing several in vitro radical scavenging/inhibiting assays and pulse radiolysis study. In addition, its ability to protect rat liver mitochondria against tertiary-butyl hydroperoxide (TBHP) and hydroxyl radical generated oxidative damage was determined by measuring the damage markers such as protein carbonyls, protein sulphydryls, lipid hydroperoxides, lipid peroxides and 8-hydroxy-2'-deoxyguanosine. Pterostilbene was also evaluated for its ability to inhibit •OH radical induced single strand breaks in pBR322 DNA.ResultPterostilbene exhibited strong anti-oxidant activity against various free radicals such as DPPH, ABTS, hydroxyl, superoxide and hydrogen peroxide in a concentration dependent manner. Pterostilbene conferred protection to proteins, lipids and DNA in isolated mitochondrial fractions against TBHP and hydroxyl radical induced oxidative damage. It also protected pBR322 DNA against oxidative assault.ConclusionsThus, present study provides an evidence for the strong anti-oxidant property of Pterostilbene, methoxylated analog of Resveratrol, thereby potentiating its role as an anti-oxidant.

show abstract

Section: Methodsmentioning

confidence: 99%

Protective effect of Pterostilbene against free radical mediated oxidative damage

Acharya

Ghaskadbi

2013

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…They are very interesting compounds due to their important applications in many pharmaceutical biological and chemical fields (De Lima et al 1994). It has also been reported that they shows antioxidative activity inhibiting lipid peroxidation (Devasagayam et al 1983) and protein oxidation (Yoshikawa et al 2000). Moreover, this compounds have been researched local anesthesical activities (Mazzone et al 1993), muscle relaxant effects, nervous system depressant (Flaherty et al 1996, Gravier et al 1992, cytotoxicity and in vitro antituberkulosis activities (Barros Costa et al 1995, Foroumadi et al 2001.…”

Section: Introductionmentioning

confidence: 99%

Effects of The Hydroxyurea Derivative 1, 3 ,4 - Thiadiazoles on Antioxidant Vitamins and MDA in Serums of Rats and Cell Viability of MCF-7 Breast Cancer Cells

Karagozoglu

2015

Journal of Cellular Neuroscience and Oxidative Stress

View full text Add to dashboard Cite

Journal of Cellular Neuroscience and Oxidative Stress is an online journal that publishes original research articles, reviews and short reviews on the molecular basis of biophysical, physiological and pharmacological processes that regulate cellular function, and the control or alteration of these processes by the action of receptors, neurotransmitters, second messengers, cation, anions, drugs or disease. Areas of particular interest are four topics. They are; A-Ion Channels (Na + -K + Channels, Clchannels, Ca 2+ channels, ADP-Ribose and metabolism of NAD + , Patch-Clamp applications) B-Oxidative Stress (Antioxidant vitamins, antioxidant enzymes, metabolism of nitric oxide, oxidative stress, biophysics, biochemistry and physiology of free oxygen radicals) C-Interaction Between Oxidative Stress and Ion Channels in Neuroscience (Effects of the oxidative stress on the activation of the voltage sensitive cation channels, effect of ADP-Ribose and NAD + on activation of the cation channels which are sensitive to voltage, effect of the oxidative stress on activation of the TRP channels, role of TRPM2 channels in neurodegenerative diseases such Parkinson's and Alzheimer's diseases) D-Gene and Oxidative Stress (Gene abnormalities. Interaction between gene and free radicals. Gene anomalies and iron. Role of radiation and cancer on gene polymorphism)

show abstract

“…21 Isolation of rat liver mitochondria and exposure to oxidative stress Three months old Wistar rats (weighing about 250 ± 20 g) were used for the preparation of mitochondria using standard protocol. 22 0.25 mg/ml rat liver mitochondria were exposed to Fenton radical initiated by ascor bateFe 2+ and oxidative damage induced to the lipids was estimated by TBARS method. Malonaldehyde (MDA) one of the major end product of lipid peroxidation was estimated spectrophotometrically using TBA re agent.…”

Section: Dna Fragmentation Assaymentioning

confidence: 99%

Comprehensive Assessment of Antioxidant Activities of Apigenin Isomers: Vitexin and Isovitexin

Khole

Panat

Suryawanshi

et al. 2016

FRA

View full text Add to dashboard Cite

Introduction: Fenugreek is known to possess anti-diabetic as well as antioxidant properties known to increase with germination. Previous study from our laboratory has reported vitexin and isovitexin as major antioxidant compounds from germinated fenugreek seeds. The present study aimed to comprehensively assesses and compare their antioxidant activity. Methods: The antioxidant ability of these compounds against various radicals was checked using standard in vitro radical scavenging assays and pulse radiolysis. Protection conferred to mitochondria against oxidative damage was assessed by measuring the levels of lipid peroxides and protein sulphydryls. Protection from hydrogen peroxide induced cytotoxicity in HepG2 cells was checked by MTT assay and by measuring intracellular ROS. Their ability to modulate intracellular antioxidant status was checked both at activity and RNA level. Result: Vitexin and isovitexin exhibited differential antioxidant activity against various radicals. Vitexin was a better nitric oxide scavenger while isovitexin scavenged superoxide radicals more efficiently. Pulse radiolysis studies revealed good antioxidant activity of both compounds against short-lived radicals and affected the formation and decay of the 2-dG transient species to the same extent. In oxidatively damaged mitochondria, lipid peroxidation was inhibited significantly by isovitexin whereas vitexin prevented decrease in protein sulphydryl content more effectively. These compounds protected HepG2 cells to the same extent against hydrogen peroxide induced oxidative insult by reducing intracellular ROS and modulating the levels of antioxidant enzymes. Conclusion: The present study clearly demonstrates differential antioxidant potential of both vitexin and isovitexin and their role in mitigating oxidative stress induced damage and maintaining cellular redox homeostasis.

show abstract

Differences in lipid peroxidation of rat liver rough and smooth microsomes

Cited by 47 publications

References 25 publications

Protective effect of Pterostilbene against free radical mediated oxidative damage

Protective effect of Pterostilbene against free radical mediated oxidative damage

Effects of The Hydroxyurea Derivative 1, 3 ,4 - Thiadiazoles on Antioxidant Vitamins and MDA in Serums of Rats and Cell Viability of MCF-7 Breast Cancer Cells

Comprehensive Assessment of Antioxidant Activities of Apigenin Isomers: Vitexin and Isovitexin

Contact Info

Product

Resources

About