Differences in Lymphocyte Subsets in the Wall of High Endothelial Venules and the Lymphatics of Human Palatine Tonsils

Zidan, Mohamed; Jecker,; Pabst,

doi:10.1046/j.1365-3083.2000.00681.x

Cited by 26 publications

(21 citation statements)

References 17 publications

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“…Using unselected lymphocytes, Ly6C crosslinking increased adhesion to endothelium by one-fifth (19%), and after purification of CD8 T cells by one-third (32%). According to the literature (35) and our own in vitro experiments, CD8 T cells are two times more adherent to peripheral lymph node HEV than CD4 T cells. Therefore, the effect of Ly6C cross-linking on the adhesion of unselected lymphocytes can be more pronounced than expected on the basis of the proportion of Ly6C ϩ cells (Ϸ12-16%) among them.…”

Section: Discussionmentioning

confidence: 88%

Ly6C Induces Clustering of LFA-1 (CD11a/CD18) and Is Involved in Subtype-Specific Adhesion of CD8 T Cells

Jaakkola

Merinen

Jalkanen

et al. 2003

The Journal of Immunology

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Ly6C is a hemopoietic cell differentiation Ag found on a subset of CD8 T cells in the periphery. It is involved in target cell killing by CTLs, augments TCR-mediated activation of IL-2 and IFN-γ production in CD8 T cells, and regulates CD8 T cell homing in vivo. In this study, we show that cross-linking of Ly6C causes clustering of LFA-1 (CD11a/CD18) on the surface of CD8 T cells via a mechanism dependent on reorganization of actin cytoskeleton and intracellular protease, calpain, but not the phosphatidylinositol 3-kinase pathway. In the capillary flow-adhesion assay, Ly6C cross-linking significantly augments lymphocyte adhesion to endothelium, and this is inhibited by an Ab that blocks LFA-1 function. Furthermore, upon in vitro cross-linking and during in vivo homing into lymph nodes, Ly6C is transiently lost from cell surface but becomes re-expressed on lymph node-resident CD8 T cells. The abilities of Ly6C to induce LFA-1 clustering and to be re-expressed after signaling-associated down-regulation may be important in regulating the homing of CD8 T cells into lymph nodes and in subsequent steps of CD8 T cell activation and effector function that again involve LFA-1.

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Section: Discussionmentioning

confidence: 88%

Ly6C Induces Clustering of LFA-1 (CD11a/CD18) and Is Involved in Subtype-Specific Adhesion of CD8 T Cells

Jaakkola

Merinen

Jalkanen

et al. 2003

The Journal of Immunology

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“…Recirculating lymphocytes may migrate across the wall of these venules from the blood to the BALT similar to other lymphoid tissue (Gowans and Knight 1964, Anderson and Anderson 1976and Zidan et al, 2000 and filtered again into the associated lymphatic vessels which were observed in the margin of all BALT of the camel. This mechanism of lymphocytes recirculation were described in several lymphoid structure (Williams et al, 1995, Zidan et al, 2000and Zidan and Pabst 2008, 2012.…”

Section: Discussionmentioning

confidence: 94%

Organization of the Bronchial Associated Lymphoid Tissue (BALT) of The Dromedary Camel (Camelus Dromedarius)

Elhussieny¹,

Zidan²,

Doughbag³

et al. 2017

AJVS

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Key words: Camel, BALT, Bronchus, BronchiolesThe BALT is an organized lymphoid tissue in the mucosa of the bronchi and bronchioles that samples antigens leading to lymphocytes stimulation. This vital structure was studied in different species but not in the camel. Therefore this work studied the bronchi and bronchioles of 14 healthy camels of different ages (10 months -12 years) using light and transmission electron microscope. BALT was regularly present in all specimens studied. It was variable in locations and structures. BALT was found in the form of patches across the circumference of the bronchial tree in the form of loose lymphocytic aggregations to a well-organized lymphoid tissue with secondary lymphoid follicles. It was localized in the lamina propria and sub mucosa or even the adventitia. At the margin of BALT, several high endothelial venules and lymphatics were localized. The BALT associated epithelium was formed from ordinary pseudo-stratified columnar ciliated epithelium with goblet cells in the bronchi and simple columnar in the bronchioles. Interepithelial lymphocytic infiltration of variable number was observed.

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“…A further important contribution to the characterization of T-lymphocyte migration within the intra or extra thymus compartments derives from recent studies on high endothelial venules (HEV). Zidan et al (2000), Lacorre et al (2004), andOviedo-Orta et al (2002) have underlined the interesting morphological pattern displayed by HEV. According to Zidan et al (2000) lymphocyte trafficking plays a critical role in disseminating specifically primed lymphocytes all over the body.…”

Section: Discussionmentioning

confidence: 94%

“…Zidan et al (2000), Lacorre et al (2004), andOviedo-Orta et al (2002) have underlined the interesting morphological pattern displayed by HEV. According to Zidan et al (2000) lymphocyte trafficking plays a critical role in disseminating specifically primed lymphocytes all over the body. The same authors investigated lymphocyte subsets in the HEV wall, in the lumen of lymphatic vessels, and in human palatine tonsils by immune histology.…”

Section: Discussionmentioning

confidence: 94%

Age‐related changes in the human thymus studied with scanning electron microscopy

Cavallotti

D’Andrea

Tonnarini

et al. 2008

Microscopy Res & Technique

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This investigation describes some morphological, age-related changes in different compartments and cells of the human thymus. Scanning electron microscopic observations were done on human thymus samples obtained from four young and eight elderly patients during thoracic surgery and/or diagnostic biopsy of the thymus, after receiving authorization from the Ethical Committee of our university. The morphological data were submitted to quantitative image analysis so as to obtain quantitative results. Subsequently, the related values were used for statistical analysis. Our findings demonstrate that (1) all thymus compartments (subcapsular spaces, cortical, medullar, thymus microenvironment) contain numerous thymocytes even after the thymus has aged. (2) In older humans, residual thymus lymphoid islets contain, in addition to fatty cells and/or fibrous cells, also the same types of resident and nonresident cells (permanent and moving cells) that are found in young and adult subjects. (3) Endothelial cells of thymus microvessels contain numerous gaps. These gaps are tight in young subjects and become loose with age. (4) Thymocytes, in older subjects, are always found near these loose endothelial gaps of thymus microvessels. (5) While thymus cortical microvessels are provided with pericytes and/or periarteriolar spaces, microvessels of the thymus medullar are free of such spaces. Our morphological and quantitative results lead us to consider the possibility that the thymus fraction of resident and permanent cells (including thymocytes and reticular epithelial cells) is larger in younger subjects compared with older ones. The endothelial loose gaps of thymus microvessels, in older subjects, can allow the bidirectional transit of thymocytes through the wall of the said microvessels.

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Differences in Lymphocyte Subsets in the Wall of High Endothelial Venules and the Lymphatics of Human Palatine Tonsils

Cited by 26 publications

References 17 publications

Ly6C Induces Clustering of LFA-1 (CD11a/CD18) and Is Involved in Subtype-Specific Adhesion of CD8 T Cells

Ly6C Induces Clustering of LFA-1 (CD11a/CD18) and Is Involved in Subtype-Specific Adhesion of CD8 T Cells

Organization of the Bronchial Associated Lymphoid Tissue (BALT) of The Dromedary Camel (Camelus Dromedarius)

Age‐related changes in the human thymus studied with scanning electron microscopy

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