Differences in major bacterial populations in the intestines of mature broilers after feeding virginiamycin or bacitracin methylene disalicylate

Neumann, Anthony P.; Suen, Garret

doi:10.1111/jam.12960

Cited by 38 publications

(36 citation statements)

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“…Many reports showed that the relative abundance of phyla Bacteroidetes increased and that of genus Lactobacillus decreased in the intestines of chickens after the administration of antibiotic 9,10,47-50 , Higher numbers of lactobacilli were previously implicated in broiler growth depression due to competition in nutrient uptake or impaired fat absorption 30,48 . Some other studies showed that proportion of phyla Firmicutes and genus of Lactobacillus increased in the gut of the chicken after administration of antibiotics [51][52][53] . Metabolomic analysis showed that 4-EOTC treatment improved the synthesis of lysophosphatidylcholine (LysoPC), as indicated by the lipid biomarkers LysoPC (16:0), LysoPC (18:3), LysoPC (20:3), and LysoPC (20:4) in the blood of Wistar rats.…”

Section: Discussionmentioning

confidence: 93%

Microbiota and metabolome responses in the cecum and serum of broiler chickens fed with plant essential oils or virginiamycin

Chen

Wang

et al. 2020

Sci Rep

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This study investigated the cecal microbiota and serum metabolite profile of chickens fed with plant essential oils (PEO) or virginiamycin (VIRG) using high-throughput 16S rRNA gene sequencing and untargeted metabolomics approach. the main aim of this work was to explore the biochemical mechanisms involved in the improved growth performance of antibiotics and their alternatives in animal production. The results showed that both PEO and VIRG treatment significantly increased the relative abundance of phyla Bacteroidetes and decreased the abundance of phyla firmicutes and genus of Lactobacillus in cecal microbiota of chickens. compared to the control group (ct group), the relative abundance of genus of Alistipes, unclassified Rikenellaceae, Roseburia, and Anaeroplasma was enriched in the peo group; that of genus Bacteroides, Lachnospiraceae, and unclassified Enterobacteriaceae was enriched in the cecal microbiota of the ViRG group. Untargeted metabolomics analyses revealed that the PEO treatment modified 102 metabolites and 3 KEGG pathways (primary bile acid biosynthesis and phenylalanine metabolism) in the cecal microbiota, and 81 metabolites and relevant KEGG pathways (fructose and mannose metabolism, biosynthesis of unsaturated fatty acids, and linoleic acid.) in the serum of the chicken. Compared to the CT group, VIRG treatment group differed 217 metabolites and 10 KEGG pathways in cecal contents and 142 metabolites and 7 KEGG pathways in serum of chickens. pearson's correlation analysis showed that phyla Bacteroidetes and genus of Bacteroides, Alistipes, and unclassified Rikenellaceae (in the VIRG and PE group) were positively correlated with many lipid metabolites. However, phyla firmicutes and genera Lactobacillus (higher in the ct group) were negatively correlated with the lipid and thymine metabolism, and positively correlated with hydroxyisocaproic acid, cytosine, and taurine. this study shows that dietary supplementation with peo and VIRG altered the composition and metabolism profile of the cecal microbiota, modified the serum metabolism profile. The intestinal microbiota, the population of microorganisms that inhabit the intestine, plays an important role in the intestinal morphology, immunity, nutrient digestion and absorption, and host health 1-3. Many studies have demonstrated that intestinal microbiota participates in many metabolic pathways, such as lipid metabolism and amino acid synthesis 4,5. The mechanism by which PEO promote growth of may be alter gut microflora and hence improved absorption of nutrients 6 , increase absorption of micronutrients in the small intestine 7 and reducing the deleterious effects of the microbial metabolites 8-10. There are many alternatives to antibiotics, such as acidifiers, probiotics, oligosaccharides, and plant extracts, which play a growth promoting role by regulating gut microbes in pig and poultry 11. Plant essential oils (PEO), which can be extracted from plants by steam distillation, extrusion, or solvent extraction 12,13 , serve as alternatives for antibi...

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Section: Discussionmentioning

confidence: 93%

Microbiota and metabolome responses in the cecum and serum of broiler chickens fed with plant essential oils or virginiamycin

Chen

Wang

et al. 2020

Sci Rep

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“…However, these limited and temporal changes may exert a significant influence on immune programming. This may be due to the fact that jejunum was chosen as the tissue of interest, which has a less complex microbiota composition and diversity compared to caecum, Shannon index 2.5 for jejunum and 5.5 for caecum [58]. We still need more knowledge about this early life phase to fully understand the gut (eco)system and its implication towards the development and programming of the immune system.…”

Section: Discussionmentioning

confidence: 99%

Perturbation of microbiota in one-day old broiler chickens with antibiotic for 24 hours negatively affects intestinal immune development

Schokker

Jansman

Veninga³

et al. 2017

BMC Genomics

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BackgroundGut microbial colonization and development of immune competence are intertwined and are influenced by early-life nutritional, environmental, and management factors. Perturbation of the gut microbiome at young age affects the crosstalk between intestinal bacteria and host cells of the intestinal mucosa.ResultsWe investigated the effect of a perturbation of the normal early life microbial colonization of the jejunum in 1-day old chickens. Perturbation was induced by administering 0.8 mg amoxicillin per bird per day) via the drinking water for a period of 24 h. Effects of the perturbation were measured by 16S rRNA profiling of the microbiome and whole genome gene expression analysis. In parallel to what has been observed for other animal species, we hypothesized that such an intervention may have negative impact on immune development.Trends were observed in changes of the composition and diversity of the microbiome when comparing antibiotic treated birds with their controls. in the jejunum, the expression of numerous genes changed, which potentially leads to changes in biological activities of the small intestinal mucosa. Validation of the predicted functional changes was performed by staining immune cells in the small intestinal mucosa and a reduction in the number of macrophage-like (KUL01+) cells was observed due to a direct or indirect effect of the antibiotic treatment. We provide evidence that a short, early life antibiotic treatment affects both the intestinal microbiota (temporarily) and mucosal gene expression over a period of 2 weeks.ConclusionThese results underscore the importance of early life microbial colonization of the gut in relation to immune development and the necessity to explore the capabilities of a variety of early life dietary and/or environmental factors to modulate the programming for immune competence in broilers.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3625-6) contains supplementary material, which is available to authorized users.

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“…The rex rabbits gastrointestinal (GI) tract is colonized by a dense microflora community that is intimately connected to the overall heath and the development of its animal host. In livestock, there is a considerable interest in understanding how this community contributes to the efficient feed conversion into animal growth (Neumann and Suen 2015 ). Improvements in feed conversion associated with dietary supplementation with antibiotics are thought to involve GI tract microbial communities, but this connection remains poorly understood (Dibner and Richards 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Illumina Miseq platform analysis caecum bacterial communities of rex rabbits fed with different antibiotics

Zou

Zeng

Wen³

et al. 2016

AMB Expr

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Antibiotics have been widely used for the prevention and the treatment of diseases to humans and animals, and they have fed additives for agricultural animals to promote growth. However, there is a growing concern over the practice due to its side effects on intestinal microbial communities which plays a vital role in animals’ health. To investigate the effect of antibiotics on the bacterial population of the caecum in rex rabbits, 80 rex rabbits were randomly divided into four groups: control group (B, basal diet), chlortetracycline group (C, 50 mg/kg), colistin sulfate group (S, 20 mg/kg) and zinc bacitracin group (Z, 40 mg/kg). Caecum microbial communities of rex rabbits from the four groups were analyzed through Illumina Miseq platform after being fed 28 days. The results showed that most obtained sequences belongs to Firmicutes followed by Bacteroidetes, and the ratio of Bacteroidetes/Firmicutes in C group (42.31 %) was higher than that in Z group (21.84 %). Zinc bacitracin supplementation caused a significant decreased of the Proteobacteria phylum and Lactobacillus spp. (P < 0.05), while the Lactobacillus spp. significantly increased in S group (P < 0.05). In addition, Ruminococcus spp., especially Ruminococcus albus were the predominant bacterial species found in both S and Z groups. The proportion of Coprococcus spp. significantly increased in Z group (P < 0.05). These findings suggested that the antibiotics used may cause significant changes in the caecum microbiota of rex rabbits, and we also found C group had a similarity caecum bacteria structure with B group which was probably due to the high levels of chlortetracycline resistance.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0273-1) contains supplementary material, which is available to authorized users.

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Differences in major bacterial populations in the intestines of mature broilers after feeding virginiamycin or bacitracin methylene disalicylate

Cited by 38 publications

References 36 publications

Microbiota and metabolome responses in the cecum and serum of broiler chickens fed with plant essential oils or virginiamycin

Microbiota and metabolome responses in the cecum and serum of broiler chickens fed with plant essential oils or virginiamycin

Perturbation of microbiota in one-day old broiler chickens with antibiotic for 24 hours negatively affects intestinal immune development

Illumina Miseq platform analysis caecum bacterial communities of rex rabbits fed with different antibiotics

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