Differences in mesenchymal stem cell cytokine profiles between MS patients and healthy donors: Implication for assessment of disease activity and treatment

Mazzanti, Benedetta; Aldinucci, Alessandra; Biagioli, Tiziana; Barilaro, Alessandro; Urbani, Serena; Pozzo, Simone Dal; Amato, Maria Pia; Siracusa, G.; Crescioli, Clara; Manuelli, Cinzia; Bosi, Alberto; Saccardi, Riccardo; Massacesi, Luca; Ballerini, Clara

doi:10.1016/j.jneuroim.2008.05.006

Cited by 70 publications

(53 citation statements)

References 35 publications

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“…In another study, MSCs isolated from MS patients exhibit the same properties of MSCs from healthy donors in terms of proliferation, phenotype, in vitro differentiation and immunosuppressive ability. 89 All together, these results support the utilization of autologous MSCs from autoimmune patients for transplantation purposes.…”

Section: Mscs and Human Experiencesupporting

confidence: 55%

Multipotent mesenchymal stromal cells for autoimmune diseases: teaching new dogs old tricks

Tyndall

Uccelli

2009

Bone Marrow Transplant

108

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Section: Mscs and Human Experiencesupporting

confidence: 55%

Multipotent mesenchymal stromal cells for autoimmune diseases: teaching new dogs old tricks

Tyndall

Uccelli

2009

Bone Marrow Transplant

108

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“…rPADs were analyzed by flow cytometry for the expression of the following conjugated MABs: CD34-PE, CD45-FITC, CD31-FITC, CD14-PE, CD90-PE, CD106-FITC (BD Pharmingen, San Diego, CA, USA), and CD105 PE (Ancell, Bayport, MN, USA), as described previously (Mazzanti et al 2008). Briefly, cells were harvested in 0 .…”

Section: Flow Cytometry Analysismentioning

confidence: 99%

Testosterone treatment improves metabolic syndrome-induced adipose tissue derangements

Maneschi¹,

Morelli²,

Filippi³

et al. 2012

Journal of Endocrinology

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We recently demonstrated that testosterone dosing ameliorated the metabolic profile and reduced visceral adipose tissue (VAT) in a high-fat diet (HFD)-induced rabbit model of metabolic syndrome (MetS). We studied the effects of HFD and in vivo testosterone dosing on VAT function and the adipogenic capacity of rabbit preadipocytes isolated from VAT of regular diet (RD), HFD, and testosterone-treated HFD rabbits. VAT was studied by immunohistochemistry, western blot, and RT-PCR. Isolated rPADs were exposed to adipocyte differentiating mixture (DIM) to evaluate adipogenic potential. Adipocyte size was significantly increased in HFD VAT compared with RD, indicating adipocyte dysfunction, which was normalized by testosterone dosing. Accordingly, perilipin, an anti-lipolytic protein, was significantly increased in HFD VAT, when compared with other groups. HFD VAT was hypoxic, while testosterone dosing normalized VAT oxygenation. In VAT, androgen receptor expression was positively associated with mRNA expression of GLUT4 (SLC2A4) (insulin-regulated glucose transporter) and STAMP2 (STEAP4) (androgen-dependent gene required for insulin signaling). In testosterone-treated HFD VAT, STAMP2 mRNA was significantly increased when compared with the other groups. Moreover, GLUT4 membrane translocation was significantly reduced in HFD VAT, compared with RD, and increased by testosterone. In DIM-exposed preadipocytes from HFD, triglyceride accumulation, adipocyte-specific genes, insulin-stimulated triglyceride synthesis, glucose uptake, and GLUT4 membrane translocation were reduced compared with preadipocytes from RD and normalized by in vivo testosterone dosing. In conclusion, testosterone dosing in a MetS animal model positively affects VAT functions. This could reflect the ability of testosterone in restoring insulin sensitivity in VAT, thus counteracting metabolic alterations.

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“…Ag specificity was tested periodically. MSCs were obtained as previously reported (5). Briefly, whole bone marrow was collected from the iliac crest in tubes containing acid citrate dextrose and centrifuged for 10 min at 700 3 g; the interface between plasma and red cell pellet (buffy coat) was recovered and diluted 1:10 in HBSS without calcium and magnesium (Euroclone, Milano, Italy) to be counted.…”

Section: Cellsmentioning

confidence: 99%

“…A Notch-Jagged1 cross-talk between MSCs and T cells may be responsible for T cell immunosuppression, and LPS stimulation may inhibit this suppression by downregulating Jagged1 expression via TLR4 (4). However, even if MSC stimulation by LPS is followed by upregulation of TLR4 expression, it does not affect the ability of MSCs to induce the differentiation of nonimmunogenic DCs (5). Conflicting results were reported for MSC/B cell interaction.…”

mentioning

confidence: 99%

Inhibition of Immune Synapse by Altered Dendritic Cell Actin Distribution: A New Pathway of Mesenchymal Stem Cell Immune Regulation

Aldinucci

Rizzetto²,

Pieri

et al. 2010

The Journal of Immunology

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Differences in mesenchymal stem cell cytokine profiles between MS patients and healthy donors: Implication for assessment of disease activity and treatment

Cited by 70 publications

References 35 publications

Multipotent mesenchymal stromal cells for autoimmune diseases: teaching new dogs old tricks

Multipotent mesenchymal stromal cells for autoimmune diseases: teaching new dogs old tricks

Testosterone treatment improves metabolic syndrome-induced adipose tissue derangements

Inhibition of Immune Synapse by Altered Dendritic Cell Actin Distribution: A New Pathway of Mesenchymal Stem Cell Immune Regulation

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