Differences in metabolism of 5-fluorouracil and 5-fluorouridine and regulation by glucosamine in human colon cancer multicell tumor spheroids

Acquisition of Human Concentrative Nucleoside Transporter 2 (hCNT2) Activity by Gene Transfer Confers Sensitivity to Fluoropyrimidine Nucleosides in Drug-Resistant Leukemia Cells

“…The differences in metabolism, mechanism of action, and favorable pharmacokinetics of 5-fluorouridine compared with other fluoropyrimidine nucleosides have contibuted to its efficacy in the treatment of superficial bladder cancers, which represent about 80% of bladder cancers (Song et al, 1997). 5-Fluorouridine and 5-fluoro-2Ј-deoxyuridine are also useful radiopharmaceuticals in tumor imaging using positron emission tomography and in spectroscopic analysis with 19 F NMR to follow their intracellular metabolism in vitro and in vivo (Chen et al, 1999). Since serum levels of diagnostic radiopharmaceuticals are typically very low, active transport will influence their cellular and tissue distribution.…”

Synthesis of FUDP-N-acetylglucosamine and FUDP-glucose inSaccharomyces cerevisiaecells treated with 5-fluorouracil

“…FUDP-glucose, FUDP-galactose, FUDP-N-acetylglucosamine, FUDP-N-galactosamine and trace amounts of FUDP-glucuronate were detected in hepatoma cells treated with 5-fluorouridine (Weckbecker & Keppler, 1984); and (v) FUDP sugars have also been characterized by 31 P-NMR spectra in extracts of small cell lung cancer cells (Pederson et al, 1994) and human colon tumor cells (Chen et al, 1999), and by 19 F-NMR in liver and tumor cells (Martino et al, 2005). All these studies show that animal cells synthesize a variety of FUDP sugar derivatives upon their exposure to 5-fluorouracil.…”