Differences in peroxidase activity and isoenzymes in embryogenic ane non-embryogenic ‘Shamouti’ orange ovular callus lines1

Kochba, J.; Lavee, S.; Spiegel-Roy, P.

doi:10.1093/oxfordjournals.pcp.a075455

Cited by 313 publications

(139 citation statements)

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“…POD activity was measured by the method described by Kochba [31]. The reaction mixture consisted of 2.9 mL 50 mM phosphate buffer (pH 5.5), 1.0 mL 2% H 2 O 2 , 1.0 mL 50 mM guaiacol, and 0.1 mL enzyme extract for 15 min at 37 • C. The same reaction mixture was boiled for 5 min as a control, then cooled with ice water immediately.…”

Section: Electrical Conductivity Assaymentioning

confidence: 99%

Changes in Seed Germination Ability, Lipid Peroxidation and Antioxidant Enzyme Activities of Ginkgo biloba Seed during Desiccation

Feng

Shen

Shi

et al. 2017

Forests

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Abstract:With the aim of investigating the antioxidant system and germinability in response to the desiccation of Ginkgo biloba seeds, they were put in a drying room (25 ± 2% relative humidity, 25 • C) for 67 days. Results showed that the germination rate remained constant when seed moisture content (MC) decreased from 48% (fresh seeds) to 45.1%. However, when MC reached 40.1%, the germination percentage decreased from 92% to 50%. A significant positive correlation was observed between the MC and seed germination percentage (r = 0.910). The electrical conductivity was significantly increased during the initial desiccation (48-45.1%). Furthermore, both the superoxide dismutase (SOD) and peroxidase (POD) activity first reduced, then elevated to peak values before they declined again. POD activity rose earlier than SOD activity, indicating that the POD reaction was more desiccation-sensitive than the SOD. Significant negative correlations were observed between the MC and malondialdehyde (MDA) content (r = −0.619) and electrical conductivity (r = −0.745). Our collective results suggest that G. biloba seeds are highly sensitive to desiccation. Excessive desiccation could reduce the antioxidant enzyme activity of G. biloba seeds and intensify membrane lipid peroxidation, which causes the consequent reduction-or even the complete loss-of seed germinability.

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Section: Electrical Conductivity Assaymentioning

confidence: 99%

Changes in Seed Germination Ability, Lipid Peroxidation and Antioxidant Enzyme Activities of Ginkgo biloba Seed during Desiccation

Feng

Shen

Shi

et al. 2017

Forests

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“…Peroxidase (POD) activity was measured using the modified method of Kochba et al (1997) with slight modifications. The crude POD enzyme was extracted in the same manner as the crude PPO enzyme.…”

Section: Enzyme Activitiesmentioning

confidence: 99%

Effect of 24-epibrassinolide treatment on the metabolism of eggplant fruits in relation to development of pulp browning under chilling stress

Gao

Kang²,

Liu³

et al. 2014

J Food Sci Technol

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This study aims to investigate the effect of 24-epibrassinolide (EBR) on the metabolism in relation to development of chilling injury-induced pulp browning of eggplant fruit. The fruits were dipped for 10 min in solutions containing 10 μmM EBR and then stored at 1°C for 15 days. Chilling injury index, weight loss, electrolyte leakage and malondialdehyde (MDA) content of control fruit increased during storage. Chilling injury improved phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD) activities, which are correlated with the increase of total phenolic content and pulp browning of eggplant fruit. The inhibition of pulp browning by EBR treatment was possibly attributed to preserving the cell membrane integrity, reducing total phenolic content, and decreasing PAL, PPO, and POD activities. These results suggest that EBR may inhibit chilling injury and pulp browning in eggplant fruit during cold storage.

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“…Peroxidase (POD, EC1.11.1.7) activity was assayed according to Kochba et al (1977). The POD reaction solution (3 ml) contained 20 mM phosphate buffer (pH 6.0), 20 mM guaiacol, 40 mM H 2 O 2 , and 40 μl enzyme extract.…”

Section: Assays Of Antioxidant Enzyme Activitymentioning

confidence: 99%

Impact of Salicylic Acid on the Antioxidant Enzyme System and Hydrogen Peroxide Production in Cucumis sativus under Chilling Stress

Zhang

Jiang

Lou

et al. 2011

Zeitschrift Für Naturforschung C

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Salicylic acid (SA) is a naturally produced compound and has been implicated to play important roles in defense of plants against diverse biotic and abiotic stresses. To understand how SA functions in the tolerance of cucumber (Cucumis sativus) to chilling stress, endogenous SA levels in two different cultivars with opposite chilling responsiveness were quantified. Membrane integrity, including malondialdehyde (MDA) content and leakage of electrolyte, was also examined in SA-pretreated cucumber plants under chilling conditions. In addition, activities of the two antioxidant enzymes peroxidase (POD) and catalase (CAT) were quantified, and hydrogen peroxide (H2O2) production was investigated histochemically in SA-treated leaves under chilling temperature. Chilling stress resulted in greater induction of SA levels in the chilling-tolerant cultivar Changchun mici in both leaves and seeds compared to the chilling-sensitive one Beijing jietou, while the former one contained higher levels of SA than the latter one in the seeds under normal conditions. Pretreatment with SA diminished the increased electrolyte leakage and MDA content caused by chilling in the leaves of both cultivars, while much less MDA and electrolyte leakage were produced in Changchun mici compared to Beijing jietou. Moreover, exogenous application of SA increased significantly the POD and CAT activities and soluble protein content. Most importantly, exogenous SA treatment could eliminate the accumulation of H2O2 in leaves and cotyledons of both cultivars caused by chilling stress. The data clearly demonstrated that the chilling-tolerant cultivar displays a higher SA level than the chilling-sensitive one, and that exogenous SA can enhance the chilling tolerance ability, which might be achieved through modulating the antioxidant system in cucumber.

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Differences in peroxidase activity and isoenzymes in embryogenic ane non-embryogenic ‘Shamouti’ orange ovular callus lines1

Cited by 313 publications

References 0 publications

Changes in Seed Germination Ability, Lipid Peroxidation and Antioxidant Enzyme Activities of Ginkgo biloba Seed during Desiccation

Changes in Seed Germination Ability, Lipid Peroxidation and Antioxidant Enzyme Activities of Ginkgo biloba Seed during Desiccation

Effect of 24-epibrassinolide treatment on the metabolism of eggplant fruits in relation to development of pulp browning under chilling stress

Impact of Salicylic Acid on the Antioxidant Enzyme System and Hydrogen Peroxide Production in Cucumis sativus under Chilling Stress

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