Differences in photoelectrocatalytic inactivation processes between E. coli and its isogenic single gene knockoff mutants: Destruction of membrane framework or associated proteins?

An, Taicheng; Sun, Hongwei; Li, Guiying; Zhao, Huijun; Wong, Po Keung

doi:10.1016/j.apcatb.2016.02.014

Cited by 38 publications

(19 citation statements)

References 39 publications

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“…It was observed that the antitrypanosomal potency of compounds with substitution in position 2 ( 3.a , 3.b and 3.f ) depended on the type of substituent and followed this order 3.f , 3.a and 3.c with reference to their polarity. Since chemical molecules alter cell growth by causing or inducing either cytoplasmic leakage [22], disruption of respiratory processes [23, 24], binding to DNA and/or inhibit the process of replication. Cell death observed herein could also be attributed to active reactive oxygen species (ROS) induced by the tested compounds [25].…”

Section: Resultsmentioning

confidence: 99%

Benzimidazole Schiff base derivatives: synthesis, characterization and antimicrobial activity

et al. 2019

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A series of Schiff bases (3.a-f) bearing benzimidazole moiety was successfully synthesized in ethanol by refluxing Oct-2-ynoic acid (1,3-dihydrobenzimidazole-2-ylidene)amide with substituted amines. Fourier transform infrared (FTIR), ultra violet light (UV-VIS), elemental analysis, proton (1 H) and carbon (13 C) nuclear magnetic resonance spectroscopy were used to characterize the newly synthesized Schiff bases. Micro dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the Schiff bases, against 14 human pathogenic bacteria (8 Gram negative and 6 Gram positive) and against 7 fungal strains (5 Aspergillus and 2 Fusarium) representatives. Antimalarial activity against Plasmodium falciparum and antitrypanosomal property against Trypanosoma brucei was studied in vitro at a single dose concentration of the Schiff bases. Cytotoxicity of the Schiff bases was assessed against human cervix adenocarcinoma (HeLa) cells. Results obtained show that the newly synthesized Schiff bases are very potent antimicrobial agents. Gram negative bacteria Klebsiella pneumonia and Escherichia coli were more affected on exposure to Compounds 3.c-f (MIC 7.8 µg/mL) which in turn exhibited more antibacterial potency than nalidixic acid reference drug that displayed MICs between 64 and 512 µg/mL against K. pneumonia and E. coli respectively. The test compounds also demonstrated high cytotoxic effect against Aspergillus flavus and Aspergillus carbonarius as they displayed MFC 7.8 and 15.6 µg/mL. Compound 3.c exhibited the highest fungicidal property from this series with MFC alternating between 7.8 and 15.6 µg/mL against the investigated strains. The malarial activity revealed Compounds 3.c and 3.d as the more potent antiplasmodial compounds in this group exhibiting 95% and 85% growth inhibition respectively. The IC 50 of Compounds 3.c and 3.d were determined and found to be IC 50 26.96 and 28.31 µg/mL respectively. Compound 3.a was the most cytotoxic agent against HeLa cells in this group with 48% cell growth inhibition. Compounds 3.c, 3.d and 3.f were biocompatible with HeLa cells and displayed low toxicity. With a very low cytotoxic effect against HeLa, compound 3.c stands out to be a very good antiparasitic agent and consideration to further evaluate the candidate drug against others cell lines is necessary.

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Section: Resultsmentioning

confidence: 99%

Benzimidazole Schiff base derivatives: synthesis, characterization and antimicrobial activity

et al. 2019

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“…Photoelectrocatalytic (PEC) oxidation process in which a small positive potential is applied on the semiconductor photoanode has proven to be more efficient than single photocatalytic process [8]. In PEC system, the application of a small external bias decreases the recombination rate of photogenerated electronhole pairs and thus increases the photocatalytic activity of the semiconductor photocatalyst [9].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of photoelectrocatalytic degradation of diclofenac with persulfate activated by Cu cathode

Liu

Zhao

Zeng

et al. 2017

Chemical Engineering Journal

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“…As can be observed in Fig. 1 c, the bacterial envelope, composed of the outer membrane and the periplasm layer, have the largest probability of being exposed to ROS attack when compared to other cellular components ( An et al., 2016 ; Sun et al., 2016 ). Fig.…”

Section: Introductionmentioning

confidence: 76%

“…For Gram-negative bacteria, such as E. coli , the outer membrane, the cell wall and the cytoplasmic membrane are located in the outer part of the cell, and thus, these parts of the microorganism are the most suitable targets for • OH radical attack ( An et al., 2016 ). In this regard, An et al.…”

Section: Quasi-direct Oxidationmentioning

confidence: 99%

Photo-assisted electrochemical advanced oxidation processes for the disinfection of aqueous solutions: A review

et al. 2021

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Disinfection is usually the final step in water treatment and its effectiveness is of paramount importance in ensuring public health. Chlorination, ultraviolet (UV) irradiation and ozone (O 3 ) are currently the most common methods for water disinfection; however, the generation of toxic by-products and the non-remnant effect of UV and O 3 still constitute major drawbacks. Photo-assisted electrochemical advanced oxidation processes (EAOPs) on the other hand, appear as a potentially effective option for water disinfection. In these processes, the synergism between electrochemically produced active species and photo-generated radicals, improve their performance when compared with the corresponding separate processes and with other physical or chemical approaches. In photo-assisted EAOPs the inactivation of pathogens takes place by means of mechanisms that occur at different distances from the anode, that is: (i) directly at the electrode’s surface (direct oxidation), (ii) at the anode’s vicinity by means of electrochemically generated hydroxyl radical species (quasi-direct), (iii) or at the bulk solution (away from the electrode surface) by photo-electrogenerated active species (indirect oxidation). This review addresses state of the art reports concerning the inactivation of pathogens in water by means of photo-assisted EAOPs such as photo-electrocatalytic process, photo-assisted electrochemical oxidation, photo-electrocoagulation and cathodic processes. By focusing on the oxidation mechanism, it was found that while quasi-direct oxidation is the preponderant inactivation mechanism, the photo-electrocatalytic process using semiconductor materials is the most studied method as revealed by numerous reports in the literature. Advantages, disadvantages, trends and perspectives for water disinfection in photo-assisted EAOPs are also analyzed in this work.

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Differences in photoelectrocatalytic inactivation processes between E. coli and its isogenic single gene knockoff mutants: Destruction of membrane framework or associated proteins?

Cited by 38 publications

References 39 publications

Benzimidazole Schiff base derivatives: synthesis, characterization and antimicrobial activity

Benzimidazole Schiff base derivatives: synthesis, characterization and antimicrobial activity

Enhancement of photoelectrocatalytic degradation of diclofenac with persulfate activated by Cu cathode

Photo-assisted electrochemical advanced oxidation processes for the disinfection of aqueous solutions: A review

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