Differences in resistance profiles and virulence genes among methicillin-resistant and methicillin-susceptible Staphylococcus aureus of different lineages at a public tertiary hospital

Bride, Lais de Lima; Pereira, Monalessa Fábia; Barbosa, Maralisi Coutinho; Silva, Nayara Carvalho; Klein, Nazareth Magnago; Nascimento, Thiago César; Schuenck, Ricardo Pinto

doi:10.1590/0037-8682-0095-2019

Cited by 9 publications

(7 citation statements)

References 31 publications

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“…In the present study, 177 MRSA (81.6%) isolates exhibited resistance to at least one of the non-beta-lactam antimicrobials tested, which is indicative of the high resistance rates for erythromycin, ciprofloxacin, and clindamycin antimicrobials. These resistance rates are in accordance with findings from other studies in southern [28][29][30] and other regions of Brazil 31,32 .…”

Section: Discussionsupporting

confidence: 93%

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Resistance profile to antimicrobials agents in methicillin-resistant Staphylococcus aureus isolated from hospitals in South Brazil between 2014-2019

Rossato

Primon-Barros

Rocha

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen causing healthcare-associated infections. Owing to the restricted use of beta-lactams in MRSA infections, non-beta-lactam antimicrobials are required for treatment. However, MRSA can develop resistance mechanisms to non-beta-lactam antimicrobials, which reduces viable treatment options. Here, we evaluated the antimicrobial susceptibility and resistance genes of MRSA isolated from hospitalized patients in South Brazil. Methods: The antimicrobial susceptibilities of hospital MRSA (217) isolates were determined by disk diffusion or microdilution methods. Additionally, the presence of 14 resistance genes and SCCmec typing was performed by PCR. Results: Among the antimicrobials tested, we observed high erythromycin (74.2%), ciprofloxacin (64.5%), and clindamycin (46.1%) resistance rates and complete susceptibility to linezolid and vancomycin. Seventeen different patterns of MRSA antimicrobial resistance were observed, of which 42.9% represented multidrug resistance. Among erythromycin-resistant MRSA, 53.4%, 45.3%, 37.9%, 13.0%, and 6.8% carried ermA, msrA, msrB, ermC, and ermB genes, respectively. Among clindamycin-resistant MRSA, 83%, 17%, 10%, 4%, and 2% carried ermA, ermC, ermB, linA, and linB genes, respectively. Among gentamicin-resistant MRSA, 96.8%, 83.9%, and 9.7% carried aac(6')/aph(2''), aph(3')-IIIa, and ant(4')-Ia genes, respectively. Among tetracycline-resistant MRSA, 6.5% and 93.5% carried tetK and tetM genes, respectively. Lastly, among trimethoprim/sulfamethoxazole-resistant MRSA, 13.3% and 100% carried dfrA and dfrG genes, respectively. The SCCmec type IV isolates were detected more frequently, whereas the SCCmec type III isolates exhibited higher multidrug resistance. Conclusions: The study data provides information regarding the MRSA resistance profile in South Brazil that is associated with the clinical conditions of patients and can contribute to clinical decision-making.

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Section: Discussionsupporting

confidence: 93%

“…In this study, the rates for trimethoprim-sulfamethoxazole, tetracycline, and gentamicin resistance were observed to be low, which was consistent with recent reports from other studies conducted in Brazil 29,31 .…”

Section: Discussionsupporting

confidence: 92%

Resistance profile to antimicrobials agents in methicillin-resistant Staphylococcus aureus isolated from hospitals in South Brazil between 2014-2019

Rossato

Primon-Barros

Rocha

et al. 2020

Rev. Soc. Bras. Med. Trop.

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“…carriers are asymptomatic and related the process of infection is usually associated with some factor which decreases the immune response of the individual, as invasive medical procedures, being this microorganism referred to as a main agents of bacteremia, often acquired in hospital environment (Muldrew et al, 2008). Among the users of central vascular catheter, S. aureus and CoNS are the more frequent microorganisms, accounting for 30% of the isolates (Muldrew et al, 2008Montassier, et al, 2013 in our study, the most frequent samples were blood culture and catheter tip with percentages of respectively 33,6% and 17,2% for the UH and 25,5% and 13,7% for OH, similar a study in public hospital in Brazil (Bride et al, 2019).…”

Section: Discussionsupporting

confidence: 87%

“…The frequency of isolation of S. aureus and its relationship with hospital infections reach high values in many Brazilian hospitals, related of prevalence of MRSA strains varies from 40 to 80% (Lina et al, 1999b;Jones et al, 2013;Bride et al, 2019) and data from the Antimicrobial Surveillance Program show that MRSA reached 31% among the nosocomial and communitarian infections agents and are considered the more common among the most prevalent pathogens (Jones et al, 2013;Silveira et al, 2015). A survey in Brazilian hospitals showed 80% (Silveira et al, 2015) resistance of CoNS in blood cultures.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Virulence of Staphylococcus spp. in patients from oncologic and non oncologic hospitals of Recife City/PE

Rabelo

Silva

Pereira

et al. 2021

RSD

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Staphylococcus spp. is one of the major infection-associated bacteria within health care, especially in intensive care units, and one of principal cause of complication in cancer patients. This study compared the antimicrobial susceptibility profile and frequency of resistance (mecA, blaZ, ermA and ermC) and virulence (icaA, icaD and hlg) genes in Staphylococcus spp. from patients of Oncology Hospital (OH) and University Hospital (UH). The type of the ccr complex was assessed by PCR among the mecA positive isolates from the UH. Higher percentage of susceptible isolates, except for oxacillin and cefoxitin was found among the UH isolates and 27,3% vancomycin-resistant isolates were identified through the screening spot; 41 isolates displayed the MLSBc phenotype and five the MSLBi phenotype, and one isolate from the OH displayed the constitutive phenotype ermC gene. The ccr types I and II were identified with a higher frequency of ccr type I. No statistically significant difference was found in the frequency of the genes between the two groups of patients or in the two hospitals. Regarding the virulence genes, there was statistically significant difference when comparing the two hospitals.

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“…In our study, the ebps and fib genes were more prevalent in isolates from bacteremia. ebps was the most prevalent in the study by Bride et al, conducted with S. aureus strains isolated from healthcare-associated infections from various sources including blood [ 45 ]. Regarding the fib gene, other studies conducted with MRSA and S. aureus strains isolated from blood and wounds had a higher prevalence of this gene when compared to our study [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Biofilm Formation of Multidrug-Resistant MRSA Strains Isolated from Different Types of Human Infections

et al. 2021

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Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main pathogens causing chronic infections, mainly due to its capacity to form biofilms. However, the mechanisms underlying the biofilm formation of MRSA strains from different types of human infections are not fully understood. MRSA strains isolated from distinct human infections were characterized aiming to determine their biofilm-forming capacity, the biofilm resistance to conventional antibiotics and the prevalence of biofilm-related genes, including, icaA, icaB, icaC, icaD, fnbA, fnbB, clfA, clfB, cna, eno, ebpS, fib and bbp. Eighty-three clinical MRSA strains recovered from bacteremia episodes, osteomyelitis and diabetic foot ulcers were used. The biofilm-forming capacity was evaluated by the microtiter biofilm assay and the biofilm structure was analyzed via confocal scanning laser microscopy. The antimicrobial susceptibility of 24-h-old biofilms was assessed against three antibiotics and the biomass reduction was measured. The metabolic activity of biofilms was evaluated by the XTT assay. The presence of biofilm-related genes was investigated by whole-genome sequencing and by PCR. Despite different intensities, all strains showed the capacity to form biofilms. Most strains had also a large number of biofilm-related genes. However, strains isolated from osteomyelitis showed a lower capacity to form biofilms and also a lower prevalence of biofilm-associated genes. There was a significant reduction in the biofilm biomass of some strains tested against antibiotics. Our results provide important information on the biofilm-forming capacity of clinical MRSA strains, which may be essential to understand the influence of different types of infections on biofilm production and chronic infections.

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Differences in resistance profiles and virulence genes among methicillin-resistant and methicillin-susceptible Staphylococcus aureus of different lineages at a public tertiary hospital

Abstract: Differences in resistance profiles and virulence genes among methicillin-resistant and methicillin-susceptible Staphylococcus aureus of different lineages at a public tertiary hospital

Cited by 9 publications

References 31 publications

Resistance profile to antimicrobials agents in methicillin-resistant Staphylococcus aureus isolated from hospitals in South Brazil between 2014-2019

Resistance profile to antimicrobials agents in methicillin-resistant Staphylococcus aureus isolated from hospitals in South Brazil between 2014-2019

Antimicrobial Resistance and Virulence of Staphylococcus spp. in patients from oncologic and non oncologic hospitals of Recife City/PE

Biofilm Formation of Multidrug-Resistant MRSA Strains Isolated from Different Types of Human Infections

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