Differences in the outer membrane-related properties of the six classical Brucella species

Martín-Martín, Ana I.; Sancho, Pilar; Tejedor, Carmen; Fernández-Lago, Luis; Vizcaı́no, Nieves

doi:10.1016/j.tvjl.2010.05.021

Cited by 26 publications

(35 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Second, B. canis is highly infective and virulent for dogs, inducing pathological signs corresponding to brucellosis. Third, B. canis is much more resistant than smooth Brucella and rough mutants to low pH, complement, hydrogen peroxide, and bactericidal cationic peptides (27), all properties associated with virulence (49,50). Fourth, B. canis induces a lower proinflammatory response than rough Brucella mutants in animal models (25), a characteristic linked to its furtive strategy.…”

Section: Discussionmentioning

confidence: 99%

“…As in other brucellae, the stealth strategy followed by B. canis seems to be linked to the absence of recognition of putative pathogen-associated molecular patterns (PAMPs) as a result of significant changes in the cell envelope components that also allow the bacterium to persist for a longer time (21,(25)(26)(27)(28)(29). Moreover, a large proportion of the B. canis infecting bacteria that remain extracellularly located are killed, exposing putative intracellular PAMPs; still, the cytokine levels remain low.…”

Section: Discussionmentioning

confidence: 99%

“…This is significant, since B. canis harbors most of the virulence determinants defined for the genus, and the genome of this bacterium is 98 to 99% identical to the genomes of other virulent Brucella species. Noticeable differences in relation to other zoonotic Brucella species are the structure and biological characteristics of the B. canis cell envelope (21)(22)(23)(24)(25)(26)(27)(28)(29), features that may influence its virulent behavior.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

et al. 2015

View full text Add to dashboard Cite

e Canine brucellosis caused by Brucella canis is a disease of dogs and a zoonotic risk. B. canis harbors most of the virulence determinants defined for the genus, but its pathogenic strategy remains unclear since it has not been demonstrated that this natural rough bacterium is an intracellular pathogen. Studies of B. canis outbreaks in kennel facilities indicated that infected dogs displaying clinical signs did not present hematological alterations. A virulent B. canis strain isolated from those outbreaks readily replicated in different organs of mice for a protracted period. However, the levels of tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-12 in serum were close to background levels. Furthermore, B. canis induced lower levels of gamma interferon, less inflammation of the spleen, and a reduced number of granulomas in the liver in mice than did B. abortus. When the interaction of B. canis with cells was studied ex vivo, two patterns were observed, a predominant scattered cell-associated pattern of nonviable bacteria and an infrequent intracellular replicative pattern of viable bacteria in a perinuclear location. The second pattern, responsible for the increase in intracellular multiplication, was dependent on the type IV secretion system VirB and was seen only if the inoculum used for cell infections was in early exponential phase. Intracellular replicative B. canis followed an intracellular trafficking route undistinguishable from that of B. abortus. Although B. canis induces a lower proinflammatory response and has a stealthier replication cycle, it still displays the pathogenic properties of the genus and the ability to persist in infected organs based on the ability to multiply intracellularly. Brucellosis is a disease of animals and humans caused by members of the genus Brucella. Zoonotic species such as Brucella melitensis, Brucella abortus, and Brucella suis are facultative extracellular-intracellular stealthy pathogens that are able to overcome innate immunity at early times of infection (1-3) and at specific stages of adaptive immunity (4, 5). In addition to influencing the immune response, these Brucella species are able to circumvent the killing action of professional and nonprofessional phagocytes, transit within phagocytic vacuoles, and replicate extensively within the endoplasmic reticulum of cells (6). These properties allow the bacterium to spread throughout the reticuloendothelial system and promote chronic infection (3).There are other Brucella species that are also relevant pathogens; however, their infective strategies remain unclear and are not in tune with the solid results accepted for the previously mentioned zoonotic brucellae. Among these are Brucella canis, the etiological agent of brucellosis in dogs and a zoonotic pathogen (7). This pathogen induces a subclinical infection that may remain undiagnosed for protracted periods (8-10). B. canis invades the conjunctiva or the oronasal system or penetrates through the venereal route. Then it is distributed to different organs...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

et al. 2015

View full text Add to dashboard Cite

show abstract

“…B. melitensis may occur as either smooth or rough variants depending on the expression of O-Polysaccharides (OPS) as a component of the bacterial outer membrane LPS (Martin-Martin et al, 2011;Akhtar et al, 2012). The smooth LPS (S-LPS) are composed of three domains: the lipid A, the core oligosaccharide and the immunodominant portion of the molecule-the O side chain, also called the O antigen (Seyed et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

CLINICAL AND REPRODUCTIVE PATHOLOGICAL CHANGES ASSOCIATED WITH <i>BRUCELLA MELITENSIS</i> AND ITS LIPOPOLYSACCHARIDES IN FEMALE MICE VIA ORAL INOCULATION

Abdullah

Adamu

Hazirah

et al. 2013

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

Brucella melitensis (B. melitensis) are Gram-negative, aerobic, facultative intracellular bacteria that cause brucellosis that usually leads to abortion in sheep and goats. Three groups of equal number of 24 healthy female mice were used as animal models. They were orally inoculated with 0.4 mL of phosphate Buffered Saline (PBSControl group), 0.4 mL of 10 9 cfu of B. melitensis and 0.4 mL of Lipopolysaccharides (LPS) extracted from 10 9 cfu of B. melitensis (both as treatment groups). Clinical signs exhibited by the mice were observed for 10 days, after which the survived mice were euthanized by cervical dislocation. Following that, post mortem was conducted and histopathological study of the reproductive organs was carried out. B. melitensis group showed mild clinical signs compared to LPS group which showed normal behaviours except for mild ruffled fur, 14 and 34 h post-inoculation, respectively. The control group (PBS) showed normal behaviours. Histopathology results revealed that both B. melitensis and LPS groups showed mild to moderate infiltration of inflammatory cells in the reproductive organs, along with normal to mild findings of necrosis. Mild to moderate haemorrhage were found in the mice of B. melitensis group, while LPS group showed normal to mild haemorrhage and moderate to severe congestion of the ovary. The study proved that mice infected orally with B. melitensis developed mild clinical signs whereas mice orally inoculated by its LPS showed normal behavior except for the mild ruffled fur. Moreover, both groups of mice inoculated with B. melitensis immunogens developed pathological changes in the reproductive organs. The LPS of B. melitensis could be a potential candidate for the development of vaccines.

show abstract

“…This effect correlated with an increase in negative surface charge (Zeta potential, Figure 5). We also studied whether the resistance to complementmediated killing in normal serum typical of brucellae was affected by the mutations [36]. We found that, whereas the parental strain B. ovis PA, BoΔwadA and the sibling revertant strain were resistant to non-immune serum (survival percentages of 93.0 ± 5.5, 92.0 ± 4.8, 101 ± 6.9, respectively), BoΔwadB and BoΔwadC mutants were significantly (p < 0.05) affected (survival percentages of 74.0 ± 3.9 and 68.0 ± 3.2, respectively).…”

Section: B Ovis Wadb and Wadc Are Required For The Synthesis Of A Comentioning

confidence: 99%

Mutants in the lipopolysaccharide of

Soler-Lloréns

Gil-Ramírez

Zabalza-Baranguá

et al. 2014

Vet Res

Self Cite

View full text Add to dashboard Cite

Brucella spp. are Gram-negative bacteria that behave as facultative intracellular parasites of a variety of mammals. This genus includes smooth (S) and rough (R) species that carry S and R lipopolysaccharides (LPS), respectively. S-LPS is a virulence factor, and mutants affected in the S-LPS O-polysaccharide (R mutants), core oligosaccharide or both show attenuation. However, B. ovis is naturally R and is virulent in sheep. We studied the role of B. ovis LPS in virulence by mutating the orthologues of wadA, wadB and wadC, three genes known to encode LPS core glycosyltransferases in S brucellae. When mapped with antibodies to outer membrane proteins (Omps) and R-LPS, wadB and wadC mutants displayed defects in LPS structure and outer membrane topology but inactivation of wadA had little or no effect. Consistent with these observations, the wadB and wadC but not the wadA mutants were attenuated in mice. When tested as vaccines, the wadB and wadC mutants protected mice against B. ovis challenge. The results demonstrate that the LPS core is a structure essential for survival in vivo not only of S brucellae but also of a naturally R Brucella pathogenic species, and they confirm our previous hypothesis that the Brucella LPS core is a target for vaccine development. Since vaccine B. melitensis Rev 1 is S and thus interferes in serological testing for S brucellae, wadB mutant represents a candidate vaccine to be evaluated against B. ovis infection of sheep suitable for areas free of B. melitensis.

show abstract

Differences in the outer membrane-related properties of the six classical Brucella species

Cited by 26 publications

References 9 publications

Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

CLINICAL AND REPRODUCTIVE PATHOLOGICAL CHANGES ASSOCIATED WITH <i>BRUCELLA MELITENSIS</i> AND ITS LIPOPOLYSACCHARIDES IN FEMALE MICE VIA ORAL INOCULATION

Mutants in the lipopolysaccharide of

Contact Info

Product

Resources

About