Differences in the reactivity of phospholipids with FDNB in normal RBC, sickle cells and RBC ghosts

Gordesky, Stanley E.; Marinetti, G.V.; Segel, George B.

doi:10.1016/0006-291x(72)90932-1

Cited by 50 publications

(18 citation statements)

References 8 publications

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“…Our results indicated that the distribution of phospholipids in RBC membranes was discrete; PC and SM clusters were exclusively localised in the outer leaflet, whereas PE, PS/PI and PIP 2 were restricted in the inner leaflet. These results are in contrast to previous biochemical characterisations, in which each phospholipid was detected in both leaflets, although the distribution was asymmetric (Bretscher, 1972a;Bretscher, 1972b;Bütikofer et al, 1990;Devaux, 1991;Gordesky et al, 1972;van Meer et al, 1981;Verkleij et al, 1973;Zachowski, 1993). Since our method is dependent on the lipid probes employed, it is possible that lipid concentration was not sufficiently high to be detected by the probes.…”

Section: Discussioncontrasting

confidence: 89%

“…Using membrane-impermeable reagents that covalently bind to the lipid headgroup (Bretscher, 1972a;Bretscher, 1972b;Gordesky et al, 1972) or by selectively degrading the outer-leaflet phospholipids with specific phospholipases (Bütikofer et al, 1990;van Meer et al, 1981;Verkleij et al, 1973), it has been shown that 65-75% of phosphatidylcholine (PC) and ,80% of sphingomyelin (SM) are located in the outer leaflet, whereas 80-85% of phosphatidylethanolamine (PE), over 96% of phosphatidylserine (PS) and 80% of phosphatidylinositol 4,5-bisphosphate (PIP 2 ) are located in the inner leaflet of the human RBC membrane (Devaux, 1991;Zachowski, 1993).…”

Section: Introductionmentioning

confidence: 99%

“…The methods require a relatively long incubation time (10-120 min) and do not account for the possibility of lipid re-organisation during the course of the treatment. In addition, these methods are, in principle, best for single-membrane system, such as RBCs (Bretscher, 1972a;Bütikofer et al, 1990;Gordesky et al, 1972;van Meer et al, 1981;Verkleij et al, 1973) and grampositive bacteria (Rothman and Kennedy, 1977). Application of these methods to multi-membrane systems, such as eukaryotic cell membranes, requires additional measures in order to obtain highly purified membrane preparations (Chap et al, 1977;Perret et al, 1979;Post et al, 1988;Record et al, 1984;Schick et al, 1976).…”

Section: Introductionmentioning

confidence: 99%

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Transbilayer lipid distribution in nano scale

Murate

Abe

Kasahara

et al. 2015

Journal of Cell Science

110

107

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There is a limited number of methods to examine transbilayer lipid distribution in biomembranes. We employed freeze-fracture replicalabelling immunoelectron microscopy in combination with lipidbinding proteins and a peptide to examine both transbilayer distribution and lateral distribution of various phospholipids in mammalian cells. Our results indicate that phospholipids are exclusively distributed either in the outer or inner leaflet of human red blood cell (RBC) membranes. In contrast, in nucleated cells, such as human skin fibroblasts and neutrophils, sphingomyelin was distributed in both leaflets while exhibiting characteristic lipid domains in the inner leaflet. Similar to RBCs, lipid asymmetry was maintained both in resting and thrombin-activated platelets. However, the microparticles released from thrombin-activated platelets lost membrane asymmetry. Our results suggest that the microparticles were shed from platelet plasma membrane domains enriched with phosphatidylserine and/or phosphatidylinositol at the outer leaflet. These findings underscore the strict regulation and cell-type specificity of lipid asymmetry in the plasma membrane.

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Section: Discussioncontrasting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transbilayer lipid distribution in nano scale

Murate

Abe

Kasahara

et al. 2015

Journal of Cell Science

110

107

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“…Perturbations . Gascard et al (1991), Gordesky et al (1972, van Meer et al (1981) and Verkleij et al (1973).…”

Section: Introductionmentioning

confidence: 99%

Phospholipids in animal eukaryotic membranes: transverse asymmetry and movement

Zachowski

1993

Biochemical Journal

788

565

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“…In particular, the organization of amino phospholipids within the membrane, as detected by chemical probes, is abnormal (17,18). Dinitrofluorobenzene (DNFB), a probe which penetrates the membrane and reacts with amino groups on both sides of the bilayer, does not bind as I Abbreviations used in this paper: ISC, irreversibly sickled cell; PBS, phosphate-buffered saline; PC, phosphatidyl choline; PE, phosphatidyl ethanolamine; PS, phosphatidyl serine; RBC, erythrocyte; RSC, reversibly sickled cell; SMI, sphingomiyelin; TNBS, trinitrobenzene sulfoniic acid.…”

Section: Introductionmentioning

confidence: 99%

Abnormalities in membrane phospholipid organization in sickled erythrocytes.

Lubin¹,

Chiu²,

Bastacky³

et al. 1981

J. Clin. Invest.

250

115

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A B S T R A C T In contrast to the wealth of infonnation concerning membrane phospholipid asymmetry in normal human erythrocytes, very little is known about membrane phospholipid organization in pathologic erythrocytes. Since the spectrin-actin lattice, which has been suggested to play an important role in stabilizing membrane phospholipid asymmetry, is abnormal in sickled erythrocytes, we determined the effects of sickling on membrane phospholipid organization. We used two enzymatic probes: bee venom phospholipase A2 and Staphylococcus aureus sphingomyelinase C, which do not penetrate the membrane and react only with phospholipids located in the outer leaflet of the bilayer. Our results suggest that the distribution of glycerophospholipids within the membrane of sickled cells is* different from that in nonsickled cells. Compared with the normal erythrocyte, the outer membrane leaflet of the deoxygenated, reversibly sickled cells (RSC) and irreversibly sickled cells (ISC) was enriched in phosphatidyl ethanolamine in addition to containing phosphatidyl serine. These changes were compensated for by a decrease in phosphatidyl choline in that layer. The distribution of sphingomyelin over the two halves of the bilayer was unaffected by sickling. In contrast to ISC, where the organization of phospholipids was abnormal under both oxy and deoxy conditions, reoxygenation of RSC almost completely restored the organization of membrane phospholipids to normal. These results indicate that the process of sickling induces an abnormality in the organization of membrane lipids in RSC which becomes permanent in ISC.

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Differences in the reactivity of phospholipids with FDNB in normal RBC, sickle cells and RBC ghosts

Cited by 50 publications

References 8 publications

Transbilayer lipid distribution in nano scale

Transbilayer lipid distribution in nano scale

Phospholipids in animal eukaryotic membranes: transverse asymmetry and movement

Abnormalities in membrane phospholipid organization in sickled erythrocytes.

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