Differences of Extracellular Cues and Ca2+ Permeable Channels in the Signaling Pathways for Inducing Amphibian Sperm Motility

Sato, Tae; Arimura, Takashi; Murata, Kenji; Kawamura, Maako; Obama, Wataru; Suzuki, Megumu; Nakauchi, Yuni; Tominaga, Akira; Morita, Masaya; Hiraoka, Kiyoshi; Takayama‐Watanabe, Eriko; Watanabe, Akihiko

doi:10.2108/zs200159

Cited by 4 publications

(4 citation statements)

References 36 publications

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“…While the calcium channel, CatSper, has been found to be the main gate for the entry of calcium ions into the mammalian sperm cell to promote activation and the acrosome reaction, the CatSper channel is absent in amphibians (Lishko and Kirichok, 2010). However, calcium signaling is still a key component in controlling amphibian sperm motility, where flagellum movement is stimulated by voltage-dependent calcium channels (Sato et al, 2021). Within caudates, calcium is one of the major ions that can be found in the jelly layers coating the outside of the newt egg, which can help activate sperm movement (Ukita et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Comparing novel sperm extenders for the internally-fertilizing tiger salamander (Ambystoma tigrinum)

Chen,

Kouba,

Songsasen

et al. 2024

Front. Amphib. Reptile Sci.

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Due to the concentrated nature and small volume of spermic milt that is typically produced from internally-fertilizing salamanders, an extender is needed to analyze the sperm for quality and quantity metrics. Techniques for diluting salamander sperm for analysis have thus far utilized low osmolality media, which are likely physiologically distinct from the salamander female reproductive tract. Application of mammalian sperm extenders could maintain salamander sperm motility and morphology in storage longer due to the similar nature of internal fertilization in both taxa. Several sperm extenders were tested including: Multipurpose Handling Medium, Sperm Washing Medium, Modified Ham’s F-10 Basal Medium (HAM’s), Refrigeration Medium, and 10% Holtfreter’s solution as a control. The experimental treatments were divided into two groups: diluted (90 mOsm, the approximate osmolality of salamander sperm) and undiluted (280-320 mOsm). Milt samples were collected from male tiger salamanders (N=11) and diluted 1:50 with each of the treatments and measured for sperm progressive motility, total motility, and normal morphology every 10 minutes for one hour. When comparing the diluted versus undiluted treatments of the same extender, there was no difference (p>0.05) for any of the response variables. In addition, we found that 10% Holtfreter’s solution is best for maintaining progressive motility, while diluted HAM’s is best for retaining total motility and normal morphology over time. Overall, we found that osmolality is not a key driver influencing tiger salamander sperm motility, but rather other factors such as ionic composition or pH could be affecting sperm form and function.

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Section: Discussionmentioning

confidence: 99%

Comparing novel sperm extenders for the internally-fertilizing tiger salamander (Ambystoma tigrinum)

Chen,

Kouba,

Songsasen

et al. 2024

Front. Amphib. Reptile Sci.

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“…The SMIS‐triggering signaling pathway is modified among some species in correlation with the reproductive modes, such as internal and arboreal fertilization (Sato et al, 2021; Yokoe et al, 2016), suggesting that the modification of the SMIS‐triggering pathway contributes to reproductive mode diversification, leading to the establishment of internal fertilization. SMIS also evolved rapidly, given that its size varies among amphibian species (Sato et al, 2021). The rapid evolution of gamete‐recognizing proteins is common among animal species (Swanson & Vacquier, 2002; Yoshida et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…In the mode of internal fertilization in urodeles, SMIS affects quiescent sperm inseminated from the sperm reservoir of the female cloaca and induces motility for the penetration of egg jelly (Watanabe et al, 2010). SMIS is present in the egg jelly or its derivative in some anurans and urodeles; and, in nonamphibian species, it may function as in the sturgeon egg coat (Ohta et al, 2010;Sato et al, 2017Sato et al, , 2021Takayama-Watanabe et al, 2012;Watanabe et al, 2010). The SMIS-triggering signaling pathway is modified among some species in correlation with the reproductive modes, such as internal and arboreal fertilization (Sato et al, 2021;Yokoe et al, 2016), suggesting that the modification of the SMIS-triggering pathway contributes to reproductive mode diversification, leading to the establishment of internal fertilization.…”

mentioning

confidence: 99%

“…SMIS is present in the egg jelly or its derivative in some anurans and urodeles; and, in nonamphibian species, it may function as in the sturgeon egg coat (Ohta et al, 2010;Sato et al, 2017Sato et al, , 2021Takayama-Watanabe et al, 2012;Watanabe et al, 2010). The SMIS-triggering signaling pathway is modified among some species in correlation with the reproductive modes, such as internal and arboreal fertilization (Sato et al, 2021;Yokoe et al, 2016), suggesting that the modification of the SMIS-triggering pathway contributes to reproductive mode diversification, leading to the establishment of internal fertilization. SMIS also evolved rapidly, given that its size varies among amphibian species (Sato et al, 2021).…”

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confidence: 99%

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Identification and characterization of sperm motility‐initiating substance‐2 gene in internally fertilizing Cynops species

et al. 2023

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Sperm motility-initiating substance (SMIS) is an oviductal protein critical for internal fertilization in urodeles. It contributes to the establishment of various reproductive modes in amphibians and is thus a unique research model for the gene evolution of gamete-recognizing ligands that have diversified among animal species. In this study, a paralogous SMIS gene, smis2, was identified via the RNA sequencing of the oviduct of the newt, Cynops pyrrhogaster. The base sequence of the smis2 gene was homologous (>90%) to that of the original smis gene (smis1), and deduced amino acid sequences of both genes conserved six cysteine residues essential for the cysteine knot motif. Furthermore, smis2 complementary DNA was identified in the oviduct of Cynops ensicauda, and the base substitution patterns also suggested that the smis gene was duplicated in the Salamandridae. Nonsynonymous/synonymous substitution ratios of smis1 and smis2 genes were 0.79 and 2.6, respectively, suggesting that smis2 gene evolution was independently driven by positive selection. Amino acid substitutions were concentrated in the cysteine knot motif of SMIS2. The smis2 gene was expressed in some organs in addition to the oviduct; in contrast, SMIS1 was only expressed in the oviduct. The SMIS2 protein was suggested to be produced and secreted at least in the oviduct and redundantly act in sperm. These results suggest that smis1 plays the original role in the oviduct, whereas smis2 may undergo neofunctionalization, which rarely occurs in gene evolution.

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Testicular Toxicity in Rats Exposed to AlCl3: a Proteomics Study

Peng

Huang

Wei

et al. 2023

Biol Trace Elem Res

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Differences of Extracellular Cues and Ca2+ Permeable Channels in the Signaling Pathways for Inducing Amphibian Sperm Motility

Cited by 4 publications

References 36 publications

Comparing novel sperm extenders for the internally-fertilizing tiger salamander (Ambystoma tigrinum)

Comparing novel sperm extenders for the internally-fertilizing tiger salamander (Ambystoma tigrinum)

Identification and characterization of sperm motility‐initiating substance‐2 gene in internally fertilizing Cynops species

Testicular Toxicity in Rats Exposed to AlCl3: a Proteomics Study

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