Differences of platelet adhesion and thrombus activation on amorphous silicon carbide, magnesium alloy, stainless steel, and cobalt chromium stent surfaces

Hansi, Christopher; Arab, Amina; Rzany, A.; Ahrens, Ingo; Bode, Christoph; Hehrlein, Christoph

doi:10.1002/ccd.21834

Cited by 50 publications

(29 citation statements)

References 23 publications

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“…On observation of the platelet adhesion on the surfaces, we can see a remarkable difference in the shape of the adhesive platelets. We observed the blood platelets on the HPM-coated surface with few agglomerations and distortions, but the platelets on the HPM non-coated surface showed swarms forming also a morphological index for activated platelet forms [43]. This result suggests that the HPM-coated surfaces have better anti-platelet adhesion capacity compared to the non-HPMcoated surfaces in the blood platelet adhesion test.…”

Section: Discussionmentioning

confidence: 71%

“…Figure 2 shows the number of blood platelets on the HPM-coated surfaces as clearly less than that on the non-HPM-treated surface in the same condition. Only a few connected blood platelets appeared on the HPM-coated surface, but many platelets on the non-HPM-coated surface had extended pseudopodium and were connected, showing a morphological index for activated platelets [43]. On observation of the platelet adhesion on the surfaces, we can see a remarkable difference in the shape of the adhesive platelets.…”

Section: Discussionmentioning

confidence: 88%

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Thromboresistant and endothelialization effects of dopamine-mediated heparin coating on a stent material surface

Bae

Park

et al. 2012

J Mater Sci: Mater Med

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Heparinization of surfaces has proven a successful strategy to prevent thrombus formation. Inspired by the composition of adhesive proteins in mussels, the authors used dopamine to immobilize heparin on a stent surface. This study aimed to assess the thromboresistant and endothelialization effects of dopamine-mediated heparin (HPM) coating on a stent material surface. The HPM was synthesized by bonding dopamine and heparin chemically. Cobalt-chromium (Co-Cr) alloy disks were first placed in the HPM solution and applied to surface stability then underwent thromboresistant tests and human umbilical vein endothelial cells (HUVEC) cytotoxicity assays. The results showed not only thromboresistant activity and a stable state of heparin on the surfaces after investigation with toluidine blue and thrombin activation assay but also proliferation of HUVEC in vitro. Studies on animals showed that the HPM-coated stent has no obvious inflammation response and increasing of restenosis rate compared to the bare metal stent (BMS) indicating good biocompatibility as well as safety in its in vivo application. Moreover, improving the endothelial cell (EC) proliferation resulted in a higher strut-covering rate (i.e., endothelialization) with shuttle-shaped EC in the HPM-coated stent group compared to that of the BMS group. These results suggest that this facile coating approach could significantly promote endothelialization and offer greater safety than the BMS for its much improved thromboresistant property. Moreover, it may offer a platform for conjugating secondary drugs such as anti-proliferative drugs.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 88%

Thromboresistant and endothelialization effects of dopamine-mediated heparin coating on a stent material surface

Bae

Park

et al. 2012

J Mater Sci: Mater Med

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“…The silicon carbide coating is purported to reduce thrombus formation 6,7 , suppress platelet activation 8,9 , stimulate endothelialization 10 , and prevent corrosion. 11 The authors of this handbook wonder if silicon carbide also cures cancer and is effective as a treatment for erectile dysfunction.…”

Section: Pharos Techniquementioning

confidence: 99%

Endovascular Treatment of Intracranial Stenosis and Vasospasm

Harrigan¹,

Deveikis²

2012

Handbook of Cerebrovascular Disease and Neurointerventional Technique

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“…This affects the performance of a broad range of cardiovascular devices such as prosthetic valves, stents, bypass pumps, and flow-assist devices [6][7][8][9][10][11][12][13][14][15][16][17][18][19], All these hardware are designed to mimic the function of the heart either totally or partially. Ventricular assist devices are increasingly being used in congestive heart failure patients and have been proven to be more effective than treatment through drug therapy alone [6][7][8][9][10][11]20].…”

Section: Introductionmentioning

confidence: 99%

Design of a Pulsatile Flow Facility to Evaluate Thrombogenic Potential of Implantable Cardiac Devices

Arjunon

Ardana

Saikrishnan

et al. 2015

Journal of Biomechanical Engineering

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Due to expensive nature of clinical trials, implantable cardiac devices should first be extensively characterized in vitro. Prosthetic heart valves (PHVs), an important class of these devices, have been shown to be associated with thromboembolic complications. Although various in vitro systems have been designed to quantify blood-cell damage and platelet activation caused by nonphysiological hemodynamic shear stresses in these PHVs, very few systems attempt to characterize both blood damage and fluid dynamics aspects of PHVs in the same test system. Various numerical modeling methodologies are also evolving to simulate the structural mechanics, fluid mechanics, and blood damage aspects of these devices. This article presents a completely hemocompatible small-volume test-platform that can be used for thrombogenicity studies and experimental fluid mechanics characterization. Using a programmable piston pump to drive freshly drawn human blood inside a cylindrical column, the presented system can simulate various physiological and pathophysiological conditions in testing PHVs. The system includes a modular device-mounting chamber, and in this presented case, a 23 mm St. Jude Medical (SJM) Regents® mechanical heart valve (MHV) in aortic position was used as the test device. The system was validated for its capability to quantify blood damage by measuring blood damage induced by the tester itself (using freshly drawn whole human blood). Blood damage levels were ascertained through clinically relevant assays on human blood while fluid dynamics were characterized using time-resolved particle image velocimetry (PIV) using a blood-mimicking fluid. Blood damage induced by the tester itself, assessed through Thrombin-anti-Thrombin (TAT), Prothrombin factor 1.2 (PF1.2), and hemolysis (Drabkins assay), was within clinically accepted levels. The hydrodynamic performance of the tester showed consistent, repeatable physiological pressure and flow conditions. In addition, the system contains proximity sensors to accurately capture leaflet motion during the entire cardiac cycle. The PIV results showed skewing of the leakage jet, caused by the asymmetric closing of the two leaflets. All these results are critical to characterizing the blood damage and fluid dynamics characteristics of the SJM Regents® MHV, proving the utility of this tester as a precise system for assessing the hemodynamics and thrombogenicity for various PHVs.

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Differences of platelet adhesion and thrombus activation on amorphous silicon carbide, magnesium alloy, stainless steel, and cobalt chromium stent surfaces

Abstract: Passive stent coatings with specific semiconducting properties such as amorphous silicon carbide or magnesium alloy reduce thrombogenicity and may improve biocompatibility of a stent platform.

Cited by 50 publications

References 23 publications

Thromboresistant and endothelialization effects of dopamine-mediated heparin coating on a stent material surface

Thromboresistant and endothelialization effects of dopamine-mediated heparin coating on a stent material surface

Endovascular Treatment of Intracranial Stenosis and Vasospasm

Design of a Pulsatile Flow Facility to Evaluate Thrombogenic Potential of Implantable Cardiac Devices

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