Different antitumor effects of quercetin, quercetin-3′-sulfate and quercetin-3-glucuronide in human breast cancer MCF-7 cells

Wu, Qiu; Needs, Paul W.; Lu, Ya-Cheng; Kroon, Paul A.; Ren, Daoyuan; Yang, Xingbin

doi:10.1039/c7fo01964e

Cited by 101 publications

(44 citation statements)

References 35 publications

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“…The chemical structure of quercetin consists of 15 carbon atoms with two aromatic rings connected by three-carbon bridges and three hydroxyl groups (−OH) in the position 3, 5, and 7 in hydroxychromen structure (rings A and C) and two in the position 3 and 4 in hydroxyphenyl structure (ring B; Lin & Zhou, 2018). At the biological level, health benefits have been attributed to quercetin (Kumar, Vijayalakshmi, & Nadanasabapathi, 2017), mainly to reduce the prevalence of chronic degenerative diseases such as cardiovascular diseases (Porcu et al, 2018;Terao, 2017), cancer (Granato et al, 2017;Wu et al, 2018), diabetes (Dhanya, Arya, Nisha, & Jayamurthy, 2017;Peng et al, 2017), and obesity (Nabavi, Russo, Daglia, & Nabavi, 2015;Ting et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Quercetin‐Loaded Zein Nanoparticles by Electrospraying and Study of In Vitro Bioavailability

Rodríguez-Félix

Del‐Toro‐Sánchez

Cinco-Moroyoqui

et al. 2019

Journal of Food Science

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Quercetin is a hydrophobic flavonoid with high antioxidant activity. However, for biological applications, the bioavailability of quercetin is low due to physiological barriers. For this reason, an alternative is the protection of quercetin in matrices of biopolymers as zein. The objective of this work was to prepare and characterize quercetin‐loaded zein nanoparticles by electrospraying and its study of in vitro bioavailability. The physicochemical parameters such as viscosity, density, and electrical conductivity of zein solutions showed a dependence of the ethanol concentration. In addition, rheological parameters demonstrated that solutions of zein in aqueous ethanol present Newtonian behavior, rebounding in the formation of nanoparticles by electrospraying, providing spherical, homogeneous, and compact morphologies, mainly at a concentration of 80% (v/v) of ethanol and of 5% (w/v) of zein. The size and shape of quercetin‐loaded zein nanoparticles were studied by transmission electron microscopy (TEM), observing that it was entrapped, distributed throughout the nanoparticle of zein. Analysis by Fourier transform‐infrared (FT‐IR) of zein nanoparticles loaded with quercetin revealed interactions via hydrogen bonds. The efficacy of zein nanoparticles to entrap quercetin was particularly high for all quercetin concentration evaluated in this work (87.9 ± 1.5% to 93.0 ± 2.6%). The in vitro gastrointestinal release of trapped quercetin after 240 min was 79.1%, while that for free quercetin was 99.2%. The in vitro bioavailability was higher for trapped quercetin (5.9%) compared to free quercetin (1.9%), than of gastrointestinal digestion. It is concluded, that the electrospraying technique made possible the obtention of quercitin‐loaded zein nanoparticles increasing their bioavailability. Practical Application This type of nanosystems can be used in the food and pharmaceutical industry. Quercetin‐loaded zein nanoparticles for its improvement compared to free quercetin can be used to decrease the prevalence of chronic degenerative diseases by increasing of the bioavailability of quercetin in the bloodstream. Other application can be as an antioxidant system in functional foods or oils to increase shelf life.

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Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Quercetin‐Loaded Zein Nanoparticles by Electrospraying and Study of In Vitro Bioavailability

Rodríguez-Félix

Del‐Toro‐Sánchez

Cinco-Moroyoqui

et al. 2019

Journal of Food Science

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“…Chlorogenic acid can induce apoptosis in A549 human lung cancer cells by down‐regulating the expression of bcl‐2 gene and up‐regulating bax and caspase‐3 genes (Yamagata, Izawa, Onodera, & Tagami, ); isorhamnetin‐3‐O‐β‐D‐glucoside showed anticancer effect on MCF‐7 human breast cancer cells and HCT‐116 human colon cancer cells (Ezzat & Motaal, ). Quercetin‐3‐O‐β‐D‐glucuronide possesses strong antitumor effects through induction of a reactive oxygen species (ROS)‐dependent apoptosis pathway in MCF‐7 cells (Wu et al, ). Study is in progress in our laboratory to address if any one of the components or the combination of the active components are responsible for the anticancer activity of AEC.…”

Section: Discussionmentioning

confidence: 99%

“…However, the result of LC-MS analysis shows that the eugenol and oleanic acid were not involved in the fraction of human colon cancer cells (Ezzat & Motaal, 2012). Quercetin-3-O-β-Dglucuronide possesses strong antitumor effects through induction of a reactive oxygen species (ROS)-dependent apoptosis pathway in MCF-7 cells (Wu et al, 2018). Study is in progress in our laboratory to address if any one of the components or the combination of the active components are responsible for the anticancer activity of AEC.…”

Section: Discussionmentioning

confidence: 99%

Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway

Chen

et al. 2019

Phytotherapy Research

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Cloves (Syzygium aromaticum), a traditional Chinese medicinal herb, displays broad biological activity. In the present study, the aqueous extract of clove (AEC) was prepared, and its anticancer affects were studied. 3‐(4,5‐Dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetra‐zolium (MTS) analysis revealed that AEC was able to inhibit cancer cell growth in vitro on several cancer cell lines; the IC50 is around 150 μg/ml for human pancreatic ASPC‐1 and human colon HT‐29 cancer cells. Treatment of the cancer cells with AEC also diminished the colony formation significantly in both human pancreatic ASPC‐1 cancer cells and human colon HT‐29 cancer cells. In vivo study revealed that AEC inhibited the tumor growth significantly in HT‐29 xenograft mice model. Transmission electron microscope, flow cytometry assay, and fluorescence microscope analysis confirmed that AEC is capable of inducing cell autophagy. Further study showed that AMPK/ULK pathway plays an important role in AEC‐induced autophagy in cancer cells. Analysis of AEC components was performed by liquid chromatograph mass spectrometer approach, and more than nine constitutes were identified in AEC fraction. The study provides evidence that AEC has potential to be developed as a novel anticancer agent or as an adjuvant in cancer chemotherapy.

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“…In the human body, Granulosa Cells Lee, Hwang, Kwon, Surh, & Park, 2010;. The treatment of MCF-7 cancer cell lines with quercetin, quercetin-3′-sulfate, and quercetin-3-glucuronide, respectively, has been shown to lead to the generation of intracellular ROS in these cells (Wu et al, 2018). High ROS production in the body has been associated with some inflammatory mediators, such as cyclooxygenase 2 (COX-2) (Onodera, Teramura, Takehara, Shigi, & Fukuda, 2015); indeed, in HT29 human colon cancer cells, 40 μM of quercetin significantly upregulated the levels of COX-2 as a ROS mediator (Raja et al, 2017).…”

Section: Effects On Oxidative Stressmentioning

confidence: 99%

Quercetin as an anticancer agent: Focus on esophageal cancer

et al. 2020

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Esophageal cancer (EC) is regarded as the sixth highest contributor to all cancer‐related mortality, worldwide. In spite of advances in the treatment of EC, currently used methods remain ineffective. Quercetin, as a dietary antioxidant, is a plant flavonol from the flavonoid group of polyphenols, and can be found in numerous vegetables, fruits, and herbs. Quercetin can affect the processes of cancer‐related diseases via cell proliferation inhibitory effects, potential apoptosis effects, and antioxidant properties. Of the various types of cancer, the use of quercetin has now become prominent in the treatment of EC. In this review, we discuss how quercetin may be an important supplement for the prevention, treatment, and management of EC, owing to its natural origin, and low‐cost relative to synthetic cancer drugs. However, most findings cited in the current study are based on in vitro and in vivo studies, and thus, further human‐based research is necessitated. Practical applications In spite of advances in the treatment of esophageal cancer, currently used methods remain ineffective, therefore, an alternative or complementary therapy is required. Quercetin, as a dietary antioxidant, can affect the processes of cancer‐related diseases via cell proliferation inhibitory effects, potential proapoptotic functions, and antioxidant properties. Quercetin may be an important supplement for the prevention, treatment, and management of EC, owing to its natural origin. The low cost of quercetin as supplement or dietary intake, relative to synthetic cancer drugs, is an advantage of the compound which should be considered.

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Different antitumor effects of quercetin, quercetin-3′-sulfate and quercetin-3-glucuronide in human breast cancer MCF-7 cells

Cited by 101 publications

References 35 publications

Preparation and Characterization of Quercetin‐Loaded Zein Nanoparticles by Electrospraying and Study of In Vitro Bioavailability

Preparation and Characterization of Quercetin‐Loaded Zein Nanoparticles by Electrospraying and Study of In Vitro Bioavailability

Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway

Quercetin as an anticancer agent: Focus on esophageal cancer

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