Different binding characteristics of dengue-2 virus to midgut of Aedes albopictus (Diptera: Culicidae) and Culex quinquefasciatus (Diptera: Culicidae)

Liu, Mei-De; Zhao, Tongyan; Dong, Yan-De; Liu, Baolin

doi:10.1303/aez.2008.49

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…quinquefasciatus midgut membrane using VOPBA. The findings of the present study therefore support the mesenteron infection barrier theory (Liu et al , ) from pathogenic and vector competence viewpoints.…”

Section: Discussionsupporting

confidence: 91%

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Understanding the mechanism of Chikungunya virus vector competence in three species of mosquitoes

Ghosh

Mullapudi

Bomanna

et al. 2019

Medical Vet Entomology

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Chikungunya virus (CHIKV) is primarily transmitted by Aedes spp. mosquitoes. The present study investigated vector competence for CHIKV in Aedes aegypti and Aedes albopictus mosquitoes found in Madurai, South India. The role of receptor proteins on midguts contributing to permissiveness of CHIKV to Aedes spp. mosquitoes was also undertaken. Mosquitoes were orally infected with CHIKV DRDE‐06. Infection of midguts and dissemination to heads was confirmed by immunofluorescence assay at different time points. A plaque assay was performed from mosquito homogenates at different time points to study CHIKV replication. Presence of putative CHIKV receptor proteins on mosquito midgut epithelial cells was detected by virus overlay protein binding assay (VOPBA). The identity of these proteins was established using mass spectrometry. CHIKV infection of Ae. aegypti and Ae. albopictus midguts and dissemination to heads was observed to be similar. A plaque assay performed with infected mosquito homogenates revealed that CHIKV replication dynamics was similar in Aedes sp. mosquitoes until 28 days post infection. VOPBA performed with mosquito midgut membrane proteins revealed that prohibitin could serve as a putative CHIKV receptor on Aedes mosquito midguts, whereas an absence of CHIKV binding protein/s on Culex quinquefasciatus midguts can partially explain the non‐permissiveness of these mosquitoes to infection.

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Section: Discussionsupporting

confidence: 91%

“…Culex quinquefasciatus is reported to be non‐permissive to dengue virus. Liu et al () demonstrated the absence of dengue virus (serotype 2) interacting proteins on Cx. quinquefasciatus midgut membrane using VOPBA.…”

Section: Discussionmentioning

confidence: 99%

Understanding the mechanism of Chikungunya virus vector competence in three species of mosquitoes

Ghosh

Mullapudi

Bomanna

et al. 2019

Medical Vet Entomology

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“…However, less emphasis has been placed on understanding these interactions in non-competent mosquito species. Studies investigating these types of host-pathogen interactions have greatly informed our understanding of factors critical to vector competence (12)(13)(14)(15)(16)(17).…”

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confidence: 99%

Detailed Analyses of Zika Virus Tropism in Culex quinquefasciatus Reveal Systemic Refractoriness

MacLeod

Dimopoulos

2020

mBio

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The role of Culex quinquefasciatus in Zika virus transmission has been debated since the epidemic of Zika occurred in the Americas in 2015 to 2016. The majority of studies have found no evidence that C. quinquefasciatus or other Culex species are competent vectors of Zika virus, and the few studies that have proposed Zika vector status for C. quinquefasciatus have relied predominantly on quantitative real-time PCR (qRT-PCR) for viral detection. We assessed the infectious range of pre- and post-epidemic Zika virus isolates in order to classify mosquito samples based on titer infectiousness and demonstrated that two strains of C. quinquefasciatus, including one previously found to be competent, are highly resistant to infection with these Zika isolates compared to Aedes aegypti and are not competent for virus transmission. Further dissection of the dynamics of Zika exposure in both A. aegypti and C. quinquefasciatus revealed that while virus transmission by C. quinquefasciatus is blocked at the levels of the midgut and salivary glands, viral RNA persists in these tissues for prolonged periods post-exposure. We assessed Zika entry dynamics in both Aedes and Culex cells, and our results suggest that Zika virus infection in Culex cells may be blocked downstream of cell entry. These findings strongly suggest that C. quinquefasciatus is not a vector of Zika virus and additionally inform the use of qRT-PCR in vector competence assays as well as our understanding of barriers to arbovirus infection in non-susceptible mosquito species. IMPORTANCE Understanding which mosquito species transmit an emerging arbovirus is critical to effective vector control. During the Zika virus epidemic in 2015 to 2016, Aedes mosquitoes were confirmed as vectors. However, studies addressing the vector status of Culex quinquefasciatus mosquitoes presented conflicting evidence and remain an outstanding source of confusion in the field. Here, we established a robust cell-based assay to identify infectious titers of Zika virus and assessed the virus titers in C. quinquefasciatus by quantitative real-time PCR (qRT-PCR). We found that while low levels of virus were detected in C. quinquefasciatus, these titers did not correspond to infectious virus, and these mosquitoes did not transmit virus in the saliva. We also present evidence that the virus may enter Culex cells before infection is disrupted. Our findings are important for future studies incriminating vector species using qRT-PCR for virus detection and offer new information on how virus transmission is blocked by mosquitoes.

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Culex-Transmitted Diseases: Mechanisms, Impact, and Future Control Strategies using Wolbachia

Madhav,

Blasdell,

Trewin

et al. 2024

Viruses

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Mosquitoes of the Culex genus are responsible for a large burden of zoonotic virus transmission globally. Collectively, they play a significant role in the transmission of medically significant diseases such as Japanese encephalitis virus and West Nile virus. Climate change, global trade, habitat transformation and increased urbanisation are leading to the establishment of Culex mosquitoes in new geographical regions. These novel mosquito incursions are intensifying concerns about the emergence of Culex-transmitted diseases and outbreaks in previously unaffected areas. New mosquito control methods are currently being developed and deployed globally. Understanding the complex interaction between pathogens and mosquitoes is essential for developing new control strategies for Culex species mosquitoes. This article reviews the role of Culex mosquitos as vectors of zoonotic disease, discussing the transmission of viruses across different species, and the potential use of Wolbachia technologies to control disease spread. By leveraging the insights gained from recent successful field trials of Wolbachia against Aedes-borne diseases, we comprehensively discuss the feasibility of using this technique to control Culex mosquitoes and the potential for the development of next generational Wolbachia-based control methods.

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Different binding characteristics of dengue-2 virus to midgut of Aedes albopictus (Diptera: Culicidae) and Culex quinquefasciatus (Diptera: Culicidae)

Cited by 3 publications

References 13 publications

Understanding the mechanism of Chikungunya virus vector competence in three species of mosquitoes

Understanding the mechanism of Chikungunya virus vector competence in three species of mosquitoes

Detailed Analyses of Zika Virus Tropism in Culex quinquefasciatus Reveal Systemic Refractoriness

Culex-Transmitted Diseases: Mechanisms, Impact, and Future Control Strategies using Wolbachia

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