Different binding pattern of antibodies to prion protein on lymphocytes from patients with sporadic Creutzfeldt–Jakob disease

Variation in concentration of prion protein in the peripheral blood of patients with variant and sporadic Creutzfeldt‐Jakob disease detected by dissociation enhanced lanthanide fluoroimmunoassay and flow cytometry

“…We are unable to explain the reason behind the finding of this reduced expression of PrP on RBCs. We did not find any evidence of a reduction in the expression of PrP on lymphocytes of patients with sCJD when compared to neurological controls in contrast to a previous report 33 . We used both 4F2 and 3F4 anti‐PrP MoAbs together for these flow cytometry studies, however, whereas the previously reported study used 3F4 alone, which does not to bind to human RBCs 29 .…”