Different characteristics of roots in the cadmium-tolerance and Cd-binding complex formation between mono- and dicotyledonous plants

Inouhe, Masahiro; Ninomiya, Satoka; Tohoyama, Hiroshi; Joho, Masanori; Murayama, Tetsuo

doi:10.1007/bf02344245

Cited by 71 publications

(46 citation statements)

References 30 publications

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“…Maize roots accumulated similar Cd 2+ concentrations in cell wall and cytoplasm fractions independently of the mycorrhizal condition, with a slightly higher proportion in the cell wall fraction. These results are comparable to those observed by Inouhe et al (1994), who reported that cereal roots accumulated Cd almost equally in both fractions. The AMF extra-radical hyphae can transport Cd from soils to host roots (Joner and Leyval, 1997), modifying metal uptake by plants and influencing plant responses towards excessive metal concentrations (Joner and Leyval, 2001).…”

Section: Discussionsupporting

confidence: 91%

“…Fractionation of root cytoplasm and cell wall for determination of Cd content in each fraction was performed according to Inouhe et al (1994) with modifications. Roots frozen in liquid nitrogen (0.5-1.0 g FW) were homogenized using a mortar and pestle in 20 mmol L -1 Tris-HCl buffer (pH 7.8).…”

Section: Methodsmentioning

confidence: 99%

“…The critical Cd level in nutrient solution for conventional crop plants is reported to be 8 µmol L -1 (Yang et al, 1995). Nevertheless, cereals such as maize, rice and barley tolerate Cd as much as 100 µmol L -1 (Inouhe et al, 1994). In Cd-enriched soils, plants may accumulate 20 mg Cd kg -1 in their shoots.…”

Section: Introductionmentioning

confidence: 99%

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Mycorrhiza influence on maize development under Cd stress and P supply

Andrade

Silveira

2008

Braz. J. Plant Physiol.

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The role of arbuscular mycorrhizal fungi (AMF) on cadmium (Cd) accumulation and on the possible attenuation of Cd stress was studied in maize plants (Zea mays L. var. Exceller). Plants inoculated or not with Glomus macrocarpum were exposed to Cd (0-20 µmol L -1 ), at two P levels (5 and 10 mg L -1 ) in the nutrient solution. The experiment was conducted in a hydroponic system, using a randomized 2 x 2 x 2 factorial design. The mycorrhiza-Cd interaction on plant growth, nutrients and Cd accumulation, AMF root colonization and on extra-radical mycelium was investigated. Mycorrhiza promoted plant growth whereas Cd addition reduced plant biomass production. No difference in plant Cd concentrations was found between mycorrhizal (M) and non-mycorrhizal (NM) plants, where Cd accumulated mainly in roots. In general, roots showed a slightly higher Cd concentration in the cell wall than in the cytoplasmic fraction, with M roots presenting 26% more Cd in the cell wall fraction than NM roots. Mycorrhizal plants showed higher P/Cd, N/Cd and S/Cd ratios in shoots and roots compared to NM plants. Mycorrhizal colonization and the length of extra-radical mycelium were diminished by Cd addition, the reduction being more pronounced under high-P supply. Addition of Cd induced guaiacol peroxidase (GPOX) activity in roots; however, M plants, in addition to the higher root protein contents, showed no induction of GPOX activity in the presence of Cd, suggesting higher tolerance to Cd. It is concluded that Cd affected mycorrhizal symbiosis by decreasing root colonization and the development of the extraradical mycelium. Nevertheless, the higher growth and nutrients/Cd ratios observed in M plants indicate an efficient symbiosis capable of alleviating Cd stress. Key words: arbuscular mycorrhizal fungi, Cd distribution, extra-radical mycelium, heavy metals, peroxidase, phosphorus Micorriza influencia o desenvolvimento do milho sob estresse de Cd e suprimento de P: A influência dos fungos micorrízicos arbusculares (FMA) no acúmulo e na atenuação potencial do estresse causado pelo Cd foi estudada em plantas de milho (Zea mays L. var. Exceller). Plantas associadas ou não com o FMA Glomus macrocarpum foram expostas ao Cd (0-20 mmol L -1 ) em dois níveis de P (5 e 10 mg L -1 ), em solução nutritiva. O experimento foi conduzido em sistema hidropônico, com delineamento totalmente casualizado, em esquema fatorial 2 x 2 x 2. Avaliou-se o efeito da interação micorriza-Cd sobre o crescimento da planta, o acúmulo de Cd e de nutrientes, a colonização micorrízica da raiz e a produção de micélio extra-radical. A micorriza promoveu o crescimento da planta enquanto a adição de Cd reduziu a produção de biomassa vegetal. Não foram observadas diferenças nas concentrações de Cd de plantas micorrizadas (M) e não-micorrizadas (NM), acumulando-se o Cd principalmente nas raízes. Em geral, as raízes mostraram maior concentração de Cd na fração da parede celular do que na citoplasmática, sendo que raízes de plantas M apresentaram quantidade de Cd 26% maior na fração...

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Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

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Mycorrhiza influence on maize development under Cd stress and P supply

Andrade

Silveira

2008

Braz. J. Plant Physiol.

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“…In our study, roots accumulate much greater amounts of Cd than shoots (Tables 1 and 4) and dicotyledonous absorb more Cd from solution than monocotyledon (Inouhe et al 1994;Kuboi et al 1986). The root of maize showed an improved tolerance than the other plants.…”

Section: Discussionsupporting

confidence: 45%

Cadmium uptake kinetics and plants factors of shoot Cd concentration

Stritsis

Claassen

2012

Plant Soil

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Background and aims Accumulation of Cd in the shoots of plants grown on Cd contaminated soils shows considerable variation. A previous preliminary experiment established that one major reason for this variation was the rate of Cd influx into the roots (mol Cd cm −2 root s −1 ). However, this experiment did not distinguish between solubilization of soil Cd on the one hand and difference in Cd uptake kinetics on the other. The main objectives of the present study were thus to characterize Cd uptake kinetics of plants continuously exposed to Cd concentrations similar to those encountered in soils. Furthermore we determined the factors responsible for differences in shoot Cd concentration such as net Cd influx, root areashoot dry weight ratio, shoot growth rate and proportion of Cd translocated to the shoot. Materials and methods Maize, sunflower, flax and spinach were grown in nutrient solution with five constant Cd concentrations varying from 0 to 1.0 μmolL −1 . Root and shoot parameters as well as Cd uptake were determined at two harvest dates and from these data Cd net influx and shoot growth rates were calculated.Results and conclusions Cadmium uptake kinetics, i.e. the net Cd influx vs. Cd solution concentration followed a straight line. Its slope is the root absorbing power, α,The α values of spinach and flax were about double that of maize and sunflower (5×10 −6 cms −1 vs. 2.5× 10 −6 cms −1 ). Spinach and flax had a 3-5 times higher shoot Cd concentration than maize and sunflower. The difference in shoot Cd concentration was partly due to the higher Cd influx but also to a higher translocation of Cd from root to shoot and also to a slower shoot growth rate.

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“…According to Chaney (1982) cadmium is an element that is not affected by so called soil-plant barriers, which means that plants tolerate this element in their tissue (and do not reveal any toxicity symptoms) in amounts that are harmful for animals consuming these plants. On the other hand Kabata-Pendias and Pendias (1993) and Inouhe et al (1994) stated that at an increased uptake cadmium mostly accumulates in the roots. According to the authors mentioned above the cadmium blockage in the roots probably involves formation of this metal bonding with sulfhydrol and with proteins forming so called phytochelatines (Kabata-Pendias and Pendias 1993).…”

Section: Resultsmentioning

confidence: 99%

Biomass yields of shoots and roots of plants cultivated in soil amended by vermicomposts based on tannery sludge and content of heavy metals in plant tissues

Gondek¹,

Filipek-Mazur²

2003

Plant Soil Environ.

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Sewage sludge application in agriculture is the simplest method of its management. Its content of organic and inorganic toxic components is a barrier to such management. Particular attention should be paid to the content of heavy metals whose presence in sewage sludge and later in soil poses hazard for plants, animals and people. The investigations aimed to determine the effect of vermicomposts obtained from tannery sludge on development of the root system and biomass of shoots as well as heavy metal concentrations in these organs. In the first year after the vermicomposts application their effect on the maize biomass increase was equal to the farmyard manure treatment but significantly worse than the mineral fertilization. The consecutive fertilizer effect of vermicomposts of tannery sludge ted on the increase in biomass of the shoots and roots of winter rape, sunflower and oats was comparable with the farmyard manure effect but notably better than the mineral fertilization. Heavy metal concentrations in individual plants were diversified; in the plants from vermicompost treatment they were as a rule lower than in the plants from mineral or farmyard manure treatment. Absorbed heavy metals accumulated primarily in the root systems, whereas the extremely high chromium content in vermicomposts did not cause its excessive accumulation in the cultivated plants.

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Different characteristics of roots in the cadmium-tolerance and Cd-binding complex formation between mono- and dicotyledonous plants

Cited by 71 publications

References 30 publications

Mycorrhiza influence on maize development under Cd stress and P supply

Mycorrhiza influence on maize development under Cd stress and P supply

Cadmium uptake kinetics and plants factors of shoot Cd concentration

Biomass yields of shoots and roots of plants cultivated in soil amended by vermicomposts based on tannery sludge and content of heavy metals in plant tissues

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