Different compact hybrid Langmuir–Blodgett‐film coatings modify biomineralization and the ability of osteoblasts to grow

Abstract

Calcium phosphates (CaPs) are biomaterials widely used in tissue regeneration with outstanding biological performance. Although the tremendous improvements achieved in CaP's materials research over the years, their interaction with physiological environments still need to be fully understood. The aim of this study is to explore a biomimetic Langmuir-Blodgett (LB) membrane to template the growth of hydroxyapatite (HAp) coatings on Ti surfaces and the ability of these coatings in inducing biomineralization by osteoblasts cultured in vitro. Changing the phospholipids (i.e., dihexadecyl phosphate (DHP) or octadecylphosphonic acid (OPA)), we also tuned the surface Ca concentration. This structural feature gave rise to different LB-hybrid surfaces where the concentration of Ca in the OPA/HAp was higher than the concentration of Ca in DHP/HAp coating. The higher Ca amount on OPA/HAp coatings, allied to the physical-chemical features, lead to different responses on osteoblasts, stimulating or inhibiting the natural biomineralization. The OPA/HAp coating caused a delay in the osteoblast proliferation as indicated by the decrease in the cell viability at the 7th culture day. Improved cell differentiation triggered by the DHP/HAp coating resulted in higher osteoblast biomineralization. The present data underscore that besides both coatings being composed by HAp, the final interfacial composition and physical-chemical properties influence differently the osteoblast behavior. Although the best osteoblast's viability was found to OPA/HAp, our dataset attested that DHP/HAp induced mineralization more effectively than that. This unexpected finding highlight the importance of deeply understanding the biomaterial interface and suggest a promising approach to the design of biofunctional LB-based coatings with tunable properties. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2524-2534, 2018.

Fabrication and characterization of a bioactive polymethylmethacrylate‐based porous cement loaded with strontium/calcium apatite nanoparticles

“…The formation of the mineralized matrix after 14 days of culture was evaluated with the alizarin red staining methodology described elsewhere 28 . SEM images of the cells cultured on the PMMA cement were acquired after treatment of the samples with glutaraldehyde and osmium tetroxide, dehydrated with a gradient of ethanol and coated with gold 29 …”

“…28 SEM images of the cells cultured on the PMMA cement were acquired after treatment of the samples with glutaraldehyde and osmium tetroxide, dehydrated with a gradient of ethanol and coated with gold. 29…”

Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?

“…The changes of the active site orientation and its accessibility to the substrate is the main reason assigned to the reduction of activity in higher interfacial concentration of enzyme [19,26,44,45]. The enzymatic activity of TNAP produced by osteoblasts cultured on LB-films with different surface packing and different interfacial Ca 2+ concentration is also changed [46].…”

Three‐dimensional cell‐laden collagen scaffolds: From biochemistry to bone bioengineering

“…48 Biomimetic approaches have been used in order to maintain the mechanical properties of the metals simultaneously increasing their bioactivity through surface modifications. [49][50][51][52][53][54][55][56][57] The further adsorption of COL1 to titanium surfaces using biomimetic immobilization into phospholipid Langmuir-Blodgett films stimulated apatite precipitation and proliferation of osteoblasts compared to bare titanium. 58 The materials used for bone regeneration can be found as a permanent graft or a temporary tissue connector.…”