Different concentrations of Edwardsiella tarda ghost vaccine induces immune responses in vivo and protects Sparus macrocephalus against a homologous challenge

Yan, Maocang; Liu, Jinyu; Yu, Li; Wang, Xuepeng; Jiang, Heng; Hao, Fang; Guo, Zhiming; Sun, Yongcan

doi:10.1016/j.fsi.2018.06.034

Cited by 24 publications

(15 citation statements)

References 20 publications

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“…Bacterial ghosts have been reported to be important in the prevention and control of fish bacterial and viral diseases (Hossain et al., 2012; Lubitz et al., 2009; Vinod et al., 2015; Wang, Wang, Wang, Feng, et al., 2018; Wang & Lu, 2009; Yan et al., 2018). Bacterial ghosts have been shown to contain immuno‐stimulatory components, and then these antigens were phagocytized and degraded by antigen‐presenting cells such as dendritic cells and macrophages.…”

Section: Discussionmentioning

confidence: 99%

“…Non‐specific immune parameters including acid phosphatase (ACP), alkaline phosphatase (AKP), superoxide dismutase (SOD) and lysozyme (LZM) activity in sera were determined using the detection kits according to the manufacturer's protocols (Wang, Fu, et al., 2020). The phagocytic activity of leucocytes was measured using the same method as our earlier research (Yan et al., 2018). The phagocytic percentage (PP) and phagocytic index (PI) were calculated according to equations PP and PI (Yan et al., 2018).…”

Section: Methodsmentioning

confidence: 99%

“…The phagocytic activity of leucocytes was measured using the same method as our earlier research (Yan et al., 2018). The phagocytic percentage (PP) and phagocytic index (PI) were calculated according to equations PP and PI (Yan et al., 2018).…”

Section: Methodsmentioning

confidence: 99%

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Characterization ofStreptococcus iniaeghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)

Wang

Zhang

et al. 2020

Aquaculture Research

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Streptococcus iniae (S. iniae) is a Gram‐positive bacterium associated with disease in several commercial species and other animals including humans. This zoonotic pathogen has led to high worldwide morbidity and mortality and huge economic losses in aquaculture. Here a S. iniae ghost (SIG) vaccine was successfully generated with a chemical method, and its immunogenicity and protective efficacy were examined in this study. The results showed that the titres of immunoglobulin M (IgM) in serum were significantly higher in group A (SIG‐immunized) than group B (formalin‐inactivated S. iniae, FSI‐immunized), which was significantly higher than the control group (PBS‐treated). Also, the phagocytic activity was significantly higher in group A than group B, which was significantly higher than the control group. Moreover, AKP activity and lysozyme activity of serum in group A were significantly higher than group B, which was significantly higher than the control group. Also, IL‐1β, IFN‐γ and HSP70 in the head kidney of fish immunized with SIG and FSI showed significantly higher than the control group throughout the whole experimental period. In addition, IL‐1β and IFN‐γ in the spleen of fish immunized with SIG showed significantly higher than that of in the FSI‐immunized fish. Most important, the survival rate and relative per cent survival of group A were higher than group B, and both of them were all significantly more protected against the S. iniae challenge compared to the PBS‐treated group. These results suggest that immunization with SIG induces immune responses and provides protection against a virulent S. iniae challenge.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Characterization ofStreptococcus iniaeghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)

Wang

Zhang

et al. 2020

Aquaculture Research

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“…Host-bacterial interactions are very complex, the fundamental relationship between them are competition between host immunity and bacterial pathogenicity (6,(16)(17)(18). For example, host immunity after vaccination was greater than the invading ability of bacteria; fish could not be dead appearing (4,19,20). As we know E. piscicida is an important zoonotic pathogen, although Edwardsialla has been known as a serious pathogen of aquatic animals for a long time, its pathogenicity mechanisms are yet to be fully elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…Introduction capsulated, motile, intracellular bacteria, which belong to an Enterobacteriaceae, Edwardsiella species, the old name was called as E. tarda (1,2). This bacterium could infect fish and move through the food chain to infect amphibians, reptiles, and birds, and humans, which particularly infects fish and causes large economic losses (3,4). E. piscicida infected host meanly through the intestine because it provides nutrition for bacteria (5,6).…”

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confidence: 99%

Glucose 6-phosphate transport regulatory protein UhpA regulates the virulent genes in Edwardsiella piscicida

Chen¹,

Sun²,

Ye³

et al. 2020

Preprint

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Edwardsiella piscicida (E. piscicida) is an important zoonotic pathogen, which infects animals by colonizing the intestine. Glucose 6-phosphate (Glu6P) was an important carbohydrate in intestine and could be used as a regulate signal. Here we identify a virulence-regulating pathway named Glu6P transport regulatory protein UhpA, which affects the virulent genes of hemolysins, flagellar, T3SS, T6SS and metabolism related genes how to promote E. piscicida infect the host. The results showed that the metabolism related gene expression of cysteine synthase (orf 1134) and sulfate transporter (ychM) in the uhpA mutant strain ΔuhpA was 0.76-fold and 0.68-fold lower than the ones in the wild strains (P < 0.05), the gene expression of ethA and ethB in the ΔuhpA strain was 0.80-fold and 0.72-fold lower than the ones in the wild strains (P < 0.05). However, the gene expression of fliC and flgN in the ΔuhpA was 1.51-fold and 1.21-fold higher than the ones in the wild strains (P < 0.05), the gene expression of T3SS (esrB and esrC) and T6SS (evpB and evpC) in the ΔuhpA was 1.27-fold, 1.13-fold 1.28-fold and 1.23-fold higher than the ones in the wild strains (P < 0.05). Besides, the survival rate of fish challenged with E. piscicida EIB202 and ΔuhpA was 50% and 30% respectively. These suggested that although the uhpA gene deletion decreased the metabolic level and the hemolysins related gene expression in E. piscicida, the uhpA gene could down regulate the key virulent gene expression to decrease the pathogenicity of E. piscicida in fish.

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FucP mediates fucose to regulate T3SS in Edwardsiella piscicida and promotes intestinal colonization in Tilapia

et al. 2021

Aquacult Int

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Different concentrations of Edwardsiella tarda ghost vaccine induces immune responses in vivo and protects Sparus macrocephalus against a homologous challenge

Cited by 24 publications

References 20 publications

Characterization ofStreptococcus iniaeghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)

Characterization ofStreptococcus iniaeghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)

Glucose 6-phosphate transport regulatory protein UhpA regulates the virulent genes in Edwardsiella piscicida

FucP mediates fucose to regulate T3SS in Edwardsiella piscicida and promotes intestinal colonization in Tilapia

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