Different Cross-Over Electrophoretic Mobility of Factor Friuli X and Coumarin-induced Abnormal Factor X

Abstract

Lyophilized or frozen plasmas with congenital deficiencies of factors II, VII, IX, or X, abnormal factor X (factor X Friuli) plasma, absorbed normal plasma, plasmas from patients treated with coumarin anticoagulants and normal plasma were studied in a crossover electrophoresis (electrosyneresis) system using an anti-factor X antiserum. A factor X band was present in plasmas with a deficiency of factors II, VII, and IX, in abnormal factor X plasma, and in normal plasma. No difference in the position of the factor X band was noted for all these plasmas. In coumarin plasma, two factor X bands were evident. The first of these two bands had the same position as the normal factor X band; the second one migrated slightly more towards the cathode. In adsorbed normal plasma and in factor X deficient plasma, no bands were seen. Minor, lighter bands were seen in most plasmas on the cathodic side of the slide and thought to represent secondary activities of the antiserum. These results suggest that the abnormal factor X (factor X Friuli) has the same crossover electrophoretic mobility as normal factor X, whereas the coumarin-induced abnormal factor X has a different mobility.

Abnormal Factor X (Factor X Friuli) Coagulation Disorder. The Heterozygote Population

“…Further more, it was demonstrated that the cross-over electrophoresis mobility of factor X Friuli is identical to that of normal factor X and is different from the mobility shown by the coumarin-induced abnormal factor X [13,16]. Finally, it was demonstrated that no PIVK.A (prothrombin inhibitor vi tamin K absence) type inhibitor is present in Friuli plasma [17].…”

Factor X Survival and Therapeutic Factor X Levels in the Abnormal Factor X (Factor X Friuli) Coagulation Disorder

“…Furthermore, no inhibitor or PIVKA-like substance was found in Friuli plasma [12]. Finally, it was shown that the abnormal factor X has the same cross-over electrophoretic mobility as normal factor X and a different one from the coumarin-induced abnormal factor X [11,15], The patients homozygote for the factor X Friuli coagulation disorder present a moderate bleeding tendency characterized mainly by easy bruis ing, menomethrorrhagias, épistaxis, bleeding after tooth extractions and after surgical procedures. Hemarthrosis was seen only in one patient after a trauma [4-6, 9, 13, 14].…”

Factor X Friuli Coagulation Disorder

“…The condition is transmitted as an au tosomal incompletely recessive trait [10,18,21,24], The main feature of the defect consists in the presence of an abnormal factor X which may not or may be activated only very slowly by whole or partial tissue thromboplastin whereas it may still be normally activated by Russell's Viper venom [7][8][9]16]. It was also demonstrated that prothrombin is normal in Friuli patients both as activity and as antigen [13] and that no inhibitor is present [17,22], Immunologically, factor X Friuli behaves as normal factor X both in plasma and is different from coumarin-induced abnormal factor X [15,19,23],…”