Different Cytotoxic Effects of Vetiver Oil on Three Types of Cancer Cells, Mainly Targeting CNR2 on TNBC

Hanifa, Mila; Wulandari, Raditya; Zulfin, Ummi Maryam; Nugroho, Eri; Haryanti, Sari; Meiyanto, Edy

doi:10.31557/apjcp.2022.23.1.241

Cited by 8 publications

(7 citation statements)

References 38 publications

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“…On the other hand, PGV-1 targets the M phase, specifically the prometaphase (Lestari et al 2019;Meiyanto et al 2022). The different target of cell cycle inhibition could result in strong cell growth inhibition and lead to apoptosis (Suski et al 2021;Hanifa et al 2022). In this regard, we found that HST elicited modulation of apoptosis of T47D cells at 48 h. Uniquely, HST also causes cell autophagy (Lin et al 2023).…”

Section: Discussionmentioning

confidence: 75%

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Hesperitin Synergistically Promotes the Senescence Induction of Pentagamavunone-1 in Luminal Breast Cancer Cells, T47D

Rifai,

Hanifa,

Zulfin

et al. 2024

J.Tropical Biodiversity Biotechnology

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Pentagamavunone-1 (PGV-1), a curcumin analog, is a promising anticancer candidate for several cancers that have been proven in vitro and in vivo. However, the efficacy of PGV-1 against breast cancer is subject to improvement to achieve a more suitable application. Here we propose hesperitin, a citrus flavonoid, to increase the anticancer potency of PGV-1 in luminal breast cancer cells. We use the T47D cell as the model to investigate the effect of co-administration of PGV-1 and hesperitin on cell cycle block, apoptosis modulation, and senescence phenomena. PGV-1 and hesperitin showed strong and weak cytotoxicity with an IC50 value of 2 µM and 100 µM, respectively. The co-treatment of PGV-1 and hesperitin resulted in strong synergistic effects with combination index (CI) value of ≤ 0.2. This combination caused apoptosis in correlation with cell cycle disruption in G2/M phase at 48 h. In particular, PGV-1 and hesperitin combination increased the incidence of cellular senescence significantly higher than the single treatment. Despite its senescence potentiation, hesperitin did not induce senescence in normal cells. Taken together, hesperitin may increase the anticancer potency of PGV-1 by modulating cell cycle arrest and apoptosis via the senescence mechanism.

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Section: Discussionmentioning

confidence: 75%

“…Once the conversion of tetrazolium salts to blue formazan crystals was complete, as denoted by their visible presence, 100 µL of 10% sodium dodecyl sulfate (SDS) stopper was introduced, and the cells were left to incubate overnight. The measurement of absorbance at 595 nm was performed using an ELISA reader (Bio-Rad) (Hanifa et al 2022).…”

Section: Mtt Assaymentioning

confidence: 99%

Hesperitin Synergistically Promotes the Senescence Induction of Pentagamavunone-1 in Luminal Breast Cancer Cells, T47D

Rifai,

Hanifa,

Zulfin

et al. 2024

J.Tropical Biodiversity Biotechnology

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“…Ten percent of sodium dodecyl sulfate in 0.01 N HCl as the stopper reagent was added to dissolve the formed formazan. The absorbance was measured at 595 nm using a microplate reader (Hanifa, et al, 2022). The concentration that inhibits 50% of cell viability (IC 50 ) value of the agents was obtained and used for the combination assay (⅛, ¼, and ½ of IC 50 for COE or sinensetin and doxorubicin).…”

Section: Mtt (3-(4 5-dimethylthiazolyl-2)-2 5 Diphenyltetrazolium Bro...mentioning

confidence: 99%

Chromolaena odorata L. Leaf Extract Elevates Cytotoxicity of Doxorubicin on 4T1 Breast Cancer Cells

Putri,

Rahmawati,

Rahman

et al. 2024

Indones J Cancer Chemoprevent

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Chemotherapeutic agents for breast cancer such as doxorubicin can attack normal cells as the side effects. Chromolaena odorata L. and its chemical content, sinensetin, have potential anticancer and antioxidant properties. The objective of this research is to examine the anticancer properties of C. odorata leaves extract and sinensetin on 4T1 triple negative breast cancer (TNBC) cells combined with doxorubicin. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5 diphenyltetrazolium bromide) assay on 4T1 cells was used to determine the IC50 and the Combination Index (CI) of the two agents in combination. Washing out the treatment and determining the cells viability after a few days was done to evaluate the persistence of the effects to cancer cells. Chromolaena odorata extract (COE) obtained was proven to contain sinensetin which gave a positive signal on the chromatogram. COE and sinensetin were moderately cytotoxic to 4T1 cells with IC50 value of 53 μg/mL and 58 μM (21.6 μg/mL), respectively. Both compounds were synergist (CI<0.7) to strong synergist (CI<0.3) when combined with doxorubicin (IC50 90 nM = 0.05 μg/mL). COE and sinensetin exhibited moderate and not cytotoxic against Vero cells with IC50 values of 60 μg/mL and 243 μM (90.43 μg/mL), respectively. Both COE and sinensetin showed selectivity index values of >1 (1.13 and 4.19, respectively). Moreover, the cytotoxic effects of COE on 4T1 cells was persisted until 48 h after removing COE from the medium, indicating the tumor-suppression potency of COE. Our findings strengthen the scientific basis of C. odorata leaves extract to be developed as a co-chemotherapeutics agent for doxorubicin on TNBC.Keywords: Chromolaena odorata L., breast cancer cells, doxorubicin, co-chemotherapy, kidney cells.

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“…[20] Recently, essential oil has shown great potential in antitumor activities, including TNBC. [21][22][23] Whether CEO has an inhibitory effect on TNBC arouses our interest.…”

Section: Introductionmentioning

confidence: 99%

“…The overall 5‐year survival rate of TNBC is about 77 %, and the 5‐year survival rate of advanced patients is only 14 % [20] . Recently, essential oil has shown great potential in antitumor activities, including TNBC [21–23] . Whether CEO has an inhibitory effect on TNBC arouses our interest.…”

Section: Introductionmentioning

confidence: 99%

Chamomile Essential Oil: Chemical Constituents and Antitumor Activity in MDA‐MB‐231 Cells through PI3K/Akt/mTOR Signaling Pathway

Feng

Sun

et al. 2023

Chemistry & Biodiversity

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Chamomile essential oil (CEO) is extracted from chamomile and mainly used in aromatherapy. The chemical constituents and its antitumor activity on Triple-negative breast cancer (TNBC) was explored in the present study. Gas chromatography-mass spectrometry (GC/MS) was employed to analyze the chemical constituents of CEO. The cell viability, migration and invasion of TNBC cell MDA-MB-231 were measured using MTT, wound scratch and Transwell assay, respectively. The protein expression of PI3K/Akt/mTOR signaling pathway was determined by Western blot. CEO is rich in terpenoids (63.51 %), among which the identified terpenoids and their derivatives are mainly Caryophyllene (29.57 %), d-Cadinene (12.81 %), Caryophyllene oxide (14.51 %), etc. Three concentration of CEO (1, 1.5, 2 μg/mL) significantly inhibited the proliferation, migration and invasion of MDA-MB-231 cells with a dose dependent manner. Moreover, the phosphorylation of PI3K, Akt and mTOR was inhibited by CEO. The results revealed that there was abundant terpenoids in the CEO which account for 63.51 %. CEO significantly inhibited the proliferation, migration and invasion of MDA-MB-231 cells, exhibiting antitumor effect on TNBC. The antitumor effect of CEO might attribute to its inhibition on PI3K/Akt/mTOR signaling pathway. However, further study should be conducted in more TNBC cell lines and animal models to provide further evidence for TNBC treatment by CEO.

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Different Cytotoxic Effects of Vetiver Oil on Three Types of Cancer Cells, Mainly Targeting CNR2 on TNBC

Cited by 8 publications

References 38 publications

Hesperitin Synergistically Promotes the Senescence Induction of Pentagamavunone-1 in Luminal Breast Cancer Cells, T47D

Hesperitin Synergistically Promotes the Senescence Induction of Pentagamavunone-1 in Luminal Breast Cancer Cells, T47D

Chromolaena odorata L. Leaf Extract Elevates Cytotoxicity of Doxorubicin on 4T1 Breast Cancer Cells

Chamomile Essential Oil: Chemical Constituents and Antitumor Activity in MDA‐MB‐231 Cells through PI3K/Akt/mTOR Signaling Pathway

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