Different effects of H 2 O 2 treatment on cervical squamous carcinoma cells and adenocarcinoma cells

Abstract

IntroductionThis study aims to compare the antioxidant abilities of cervical squamous carcinoma cells and cervical adenocarcinoma cells and to study the related mechanisms.Material and methodsCervical squamous carcinoma and adenocarcinoma cells were treated with H2O2. Cell proliferation was determined with the MTT assay. The reactive oxygen species (ROS) level was detected by the 2’,7’-dichlorofluorescein-diacetate (DCFH-DA) method. The 5,5’-dithiobis-2-nitrobenzoic acid (DTNB) method was performed to measure intracellular concentrations of reduced glutathione (GSH) and oxidized glutathione (GSSG). The nitrite formation method, the molybdate colorimetric method, and the DTNB colorimetric method were used to determine activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), respectively.ResultsCompared with untreated control cells, cell proliferation of cervical squamous carcinoma cells and cervical adenocarcinoma cells was significantly inhibited by H2O2 treatment (p < 0.05). Reactive oxygen species levels and GSSG levels were significantly increased (p < 0.01), whereas GSH levels were significantly decreased (p < 0.05 or 0.01) in both cells after H2O2 treatment. Thus the ratio of GSH/GSSG was significantly decreased by H2O2 treatment in both cells (p < 0.01). In addition, H2O2 treatment significantly increased activities of SOD, CAT, and GPx in both cells (p < 0.05 or 0.01). Furthermore, the above-mentioned changes induced by H2O2 treatment were more dramatic in cervical squamous carcinoma cells.ConclusionsThe antioxidant ability of cervical squamous carcinoma cells is lower than that of cervical adenocarcinoma cells, which may be related to the increased ROS levels in cervical squamous carcinoma cells induced by H2O2 treatments.

Live Imaging of Label-Free Graphene Oxide Reveals Critical Factors Causing Oxidative-Stress-Mediated Cellular Responses

“…DCF-DA is a cell-permeable, nonfluorescent probe that becomes highly green-fluorescent upon oxidation with H 2 O 2 or other oxidants inside the cell . BEAS-2B cells exposed to both GOs were observed under a confocal microscope in order to accurately differentiate the signal from the oxidized probe from eventual interference of the material (Figure a and Figure S12).…”

“…DCF-DA is a cell-permeable, nonfluorescent probe that becomes highly green-fluorescent upon oxidation with H 2 O 2 or other oxidants inside the cell. 45 BEAS-2B cells exposed to both GOs were observed under a confocal microscope in order to accurately differentiate the signal from the oxidized probe from eventual interference of the material (Figure 5a and Figure S12). Bright green fluorescence distributed throughout the cell cytoplasm was clearly observed for the cells treated with s-GO w/o FBS and for the cells treated with l-GO (w/FBS or w/o FBS) (Figure 5a).…”

Inversed Expression Patterns of S100A4 and E‐Cadherin in Cervical Cancers: Implication in Epithelial–Mesenchymal Transition

“…Cervical cancer (CC) is one of commonest female cancers and the third leading cause of cancer‐related mortality among women worldwide (Jemal et al, ). The cervical squamous cell carcinomas (CSCCs) and the cervical adenocarcinomas (CACs) are the major types of cervical cancers (Zhang et al, ), which are further grouped to the well, moderately and poorly differentiated subtypes (Yoo et al, ). Among the determinants of prognoses of cervical cancers, invasiveness and metastatic capacities are the critical elements (Li et al, ).…”