Different Effects of the Immunomodulatory Drug GMDP Immobilized onto Aminopropyl Modified and Unmodified Mesoporous Silica Nanoparticles upon Peritoneal Macrophages of Women with Endometriosis

Abstract

The aim of the present work was to compare in vitro the possibility of application of unmodified silica nanoparticles (UMNPs) and modified by aminopropyl groups silica nanoparticles (AMNPs) for topical delivery of immunomodulatory drug GMDP to the peritoneal macrophages of women with endometriosis. The absence of cytotoxic effect and high cellular uptake was demonstrated for both types of silica nanoparticles. The immobilization of GMDP on the UMNPs led to the suppression of the stimulatory effect of GMDP on the membrane expression of scavenger receptors SR-AI and SR-B, mRNAs expression of NOD2 and RAGE, and synthesis of proteolytic enzyme MMP-9 and its inhibitor TIMP-1. GMDP, immobilized onto AMNPs, enhanced the initially reduced membrane expression of SRs and increased NOD2, RAGE, and MMP-9 mRNAs expression by macrophages. Simultaneously high level of mRNAs expression of factors, preventing undesirable hyperactivation of peritoneal macrophages (SOCS1 and TIMP-1), was observed in macrophages incubated in the presence of GMDP, immobilized onto AMNPs. The effect of AMNPs immobilized GMDP in some cases exceeded the effect of free GMDP. Thus, among the studied types of silica nanoparticles, AMNPs are the most suitable nanoparticles for topical delivery of GMDP to the peritoneal macrophages.

Tunable Chemical and Topographic Patterns Based on Binary Colloidal Crystals (BCCs) to Modulate MG63 Cell Growth

“…The most common types of bonds that are expected after peptide immobilization on Si particles are the amide Ι and amide ΙΙ peaks. However, as shown in Figure b, the CO stretches resulting from RGD conjugated to APTES‐treated Si5 and Si2 particles were detected between 1460 and 1590 cm −1 and are assigned to amide ΙΙ and ΙΙΙ bonds whereas peaks in the range of 1700–1750 cm −1 are a result of CO stretches overlapping on the peak for amide Ι bonds as per the previous findings . The appearance of all these bands along with a stretching and bending vibrations of APTES from the aminopropyl CH 2 groups responsible for the peaks within 2856–2940 cm −1 again confirms the successful coupling of RGD to the APTES‐treated Si particle surfaces.…”

“…However, the FTIR spectra (Figure b) of Si5 and Si2 particle surfaces did reveal some differences after particle modification. In all spectra, peaks at 795 and 1050 cm −1 can be assigned to i) SiOSi stretches while an additional two peaks were observed at 950 and 1100 cm −1 and assigned to ii) SiOH and iii) CO stretches . The PEG‐grafted Si particle surfaces showed an increment in the intensity of CO peak at 1100 cm −1 , a diminishing signal for SiOSi at 1050 cm −1 , and the emergence of a sharp single peak representing the COC stretching vibration arising from PEG chains.…”

Investigation of nanoparticle immobilized cellulase: nanoparticle identity, linker length and polyphenol hydrolysis

“…For SNPs, the peak at 3423 cm −1 is assigned to surface hydroxyl groups (stretching mode) while the band at 1635 cm −1 corresponds to O–H vibration of absorbed water [40]. Peaks at 795 cm −1 and 454 cm −1 are assigned to the stretching vibrations of the mesoporous framework (Si–O–Si) and the peak at 960 cm −1 is attributed to Si–OH bond stretching [41].…”

Letrozole and Curcumin Loaded-PLGA Nanoparticles: A Therapeutic Strategy for Endometriosis

“…In a recent study, immune modulatory drug immobilized onto mesoporous silica NPs have been tested in vitro on peritoneal macrophages of women with endometriosis [3]. In another study, polymeric micelles with glycolipid-like structure have been synthesized and investigated for targeted endometriosis gene therapy [4].…”