Different end-points to assess effects in systemic lupus erythematosus patients exposed to pesticide mixtures

Simoniello, María Fernanda; Contini, Liliana; Benavente, Emilio; Mastandrea, Carlos; Roverano, Susana; Paira, Sergio

doi:10.1016/j.tox.2016.08.003

Cited by 8 publications

(4 citation statements)

References 35 publications

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“…Given the complexity of this disease, it is necessary to search for new laboratory tests that can complement the diagnosis and/or to evaluate SLE activity and therapheutic succes. Previous studies have demonstrated increased DNA damage by alkaline comet assay in SLE (AKHTER et al, 2016;GAO et al, 2017;SIMONIELLO et al, 2017). In our study, we did not identify increased DNA damage in SLE patients in comparison to healthy individuals.…”

Section: Discussioncontrasting

confidence: 88%

“…In our cohort, 44.1% of patients presented infection/reactivation of CMV and/or EBV confirmed by qPCR. Studies have showed these infections can induce genomic instability, decrease DNA repair, and increase genetic mutations (GUTA et al, 2023;SMOLARZ;WILCZYŃSKI;NOWAKOWSKA, 2015;WU et al, 2010WU et al, , 2023. Genes stimulated by the interferon response to viral infections can affect many cellular processes, including RNA processing, protein stability, and cell viability; directly affecting essential steps in viral replication.…”

Section: Discussionmentioning

confidence: 99%

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The Assessment of Dna Damage by Alkaline Comet Assay Is Not Associated With Clinical Outcomes in Women With Systemic Lupus Erythematosus

De Melo Carvalho Nascimento,

Alves,

Cutrim Gaudio

et al. 2023

RIES

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Introduction: In systemic lupus erythematosus (SLE), an inflammatory state leads to oxidative stress and excessive formation of reactive oxygen species, increasing cellular damage, mutations and compromised DNA repair. Therefore, our study aimed to evaluate the performance of the alkaline comet assay to investigate DNA damage in women with SLE.Methods: This is a cross-sectional study that included Brazilian women with SLE attended at an university hospital (Rio de Janeiro, Brazil) from 2015 to 2018. Disease activity was assessed using the SLE Disease Activity Index 2000 (SLEDAI-2K). Diagnosis of CMVand EBV infections were performed by qPCR. DNA damage was determined by alkaline comet assay, which is a electrophoresis techinique used to evaluate DNA fragmentation in leukocytes, which is expressed as arbitrary units (AU). Results: We studied 34 SLE patients with a median age of 34.5 years-old. Hospitalized patients (n=11, 32.3%) presented higher SLEDAI-2K, where 9 (81.8%) had SLEDAI-2K ≥ 5 (p=0.038). We did not identify significant differences between healthy and SLE; however, hospitalized patients showed higher median AU, but with no statistical significance (p=0.095). Also, no differences were in DNA damage according to SLEDAI-2, lupus nephritis, use of immunossupressants, or CMV/EBV infections. DNA damage was not associated with levels of anti-dsDNA. Lastlty, ROC curves demonstrated a poor predictive power of DNA damage to differentiate groups (AUC < 0.7, p > 0,05). Conclusion: Our results indicate that DNA damage measured by alkaline comet assay is not associated with SLE severity and the presence of viral infections

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Section: Discussioncontrasting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

The Assessment of Dna Damage by Alkaline Comet Assay Is Not Associated With Clinical Outcomes in Women With Systemic Lupus Erythematosus

De Melo Carvalho Nascimento,

Alves,

Cutrim Gaudio

et al. 2023

RIES

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“…Among them, two individuals were diagnosed with either SLE or SLE-like symptoms (20), suggesting a need for more thorough examination of the potential of chlorpyrofos in inducing an autoimmune response. SLE patients exposed to pesticide mixtures and living in rural areas were found to have over 3.5 times more oxidative DNA damage than those living in the city, suggesting usefulness of DNA damage and oxidative stress in the characterization of individual risk to ADs (21). In an in vivo study, exposure to organochlorine pesticide chlordecone with estrogenic effects in ovariectomized female (NZB x NZW) F1 mice resulted in accelerated appearance of SLE disease (22).…”

Section: Pesticidesmentioning

confidence: 99%

Environmental Exposures and Autoimmune Diseases: Contribution of Gut Microbiome

2020

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Environmental agents have been gaining more attention in recent years for their role in the pathogenesis of autoimmune diseases (ADs). Increasing evidence has linked environmental exposures, including trichloroethene (TCE), silica, mercury, pristane, pesticides, and smoking to higher risk for ADs. However, potential mechanisms by which these environmental agents contribute to the disease pathogenesis remains largely unknown. Dysbiosis of the gut microbiome is another important environmental factor that has been linked to the onset of different ADs. Altered microbiota composition is associated with impaired intestinal barrier function and dysregulation of mucosal immune system, but it is unclear if gut dysbiosis is a causal factor or an outcome of ADs. In this review article, we first describe the recent epidemiological and mechanistic evidences linking environmental/occupational exposures with various ADs (especially SLE). Secondly, we discuss how changes in the gut microbiome composition (dysbiosis) could contribute to the disease pathogenesis, especially in response to exposure to environmental chemicals.

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“…After signing the informed consent to participate in the study, a face‐to‐face questionnaire was completed to obtain sociodemographic (age, place of residence), individual lifestyle (active smoking, alcohol consumption, and folic acid intake), gynecological events (parity and spontaneous abortion), and occupational risk data. The place of residence was dichotomized by urban area (Santa Fe and Rosario cities), and rural area (rest of the province), according to Simoniello et al (2017), who have previously settled the mentioned classification for this variable. Information about newborns, like diagnostics, was retrieved from the medical records available at the hospital.…”

Section: Methodsmentioning

confidence: 99%

Prenatal exposure to pesticide mixture in Argentina: A pilot study in puerperal women from Santa Fe province

Colussi,

Martinez,

Bellenger

et al. 2024

Birth Defects Research

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IntroductionThe epidemiological investigation of congenital anomalies (CA) represents a challenge due to the multiplicity of associated risk factors, notably environmental ones. The monitoring of genotoxic effects in different populations is a useful tool in human biomonitoring and has great biological importance in estimating the exposure risks to complex mixtures of chemical substances.ObjectiveThis study aimed to determine the presence of environmental xenobiotics and evaluate their genotoxic effect, in mothers of newborns with and without CA, and the possible association/correlation between those biomarkers and CA.Materials and methodsA descriptive case and control cross‐sectional study was developed on 290 postpartum women from Santa Fe, Argentina.ResultsSignificant differences were observed between both groups, for places of residence and gynecological variables. Metabolites of organochlorine (OC), organophosphate (OP), and pyrethroid (PYR) pesticides were detected. The most frequently detected compounds were atrazine (ATZ) (57.14%), carbendazim (CBZ) (46.42%), and methylparaben (46.42%), among others. A positive correlation was found between the number of pesticides in blood and genotoxic variables. On the other hand, mothers of children with genitourinary anomalies were the ones with the highest concentrations of ATZ and OP.Discussion and conclusionThese results showed a deep background in the health reality of Santa Fe, which could greatly impact the health of future adults, who have been born preterm. On the other hand, the mixture of pesticides detected confirms the environmental living conditions of women and the transplacental exposure to these compounds in each pregnancy. The potential effects on long‐term descendent health are unknown and unpredictable.

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Different end-points to assess effects in systemic lupus erythematosus patients exposed to pesticide mixtures

Cited by 8 publications

References 35 publications

The Assessment of Dna Damage by Alkaline Comet Assay Is Not Associated With Clinical Outcomes in Women With Systemic Lupus Erythematosus

The Assessment of Dna Damage by Alkaline Comet Assay Is Not Associated With Clinical Outcomes in Women With Systemic Lupus Erythematosus

Environmental Exposures and Autoimmune Diseases: Contribution of Gut Microbiome

Prenatal exposure to pesticide mixture in Argentina: A pilot study in puerperal women from Santa Fe province

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