Different pattern of interleukin-1β-(IL-1β), interleukin-1 receptor antagonist- (IL-1ra) and interleukin-1 receptor type I- (IL-1R tI) mRNA-expression in single preimplantation mouse embryos at various developmental stages

Interleukin-18 system messenger RNA and protein expression in human endometrium during the menstrual cycle

“…We have previously reported expression of the IL-1 system in single blastomeres of preimplantation human and mouse embryos (25,26). Individual embryos expressed peak IL-1 receptor antagonist mRNA expression at the blastocyst stage either cultured alone or cocultured with helper cells (24,45). Inerleukin-1␤ and transforming growth factor may play crucial roles at the embryo-maternal interface during trophoblast invasion by regulating stromal cell expression of collagenase and their inhibitors, all of which are known to be important in trophoblast invasion (31).…”

Characterization of cecal gene expression in a differentially susceptible mouse model of bacterial-induced inflammatory bowel disease

“…The sequence for HPRT, 18 claudin-4, 19 IL-1␤, 20 IL-10, 21 IL-12, 22 and MCP-2 23 primers have been previously reported in the literature. The primer sequences for IFN-␥, IP-10, MIP-1␣, MIG, and SOCS3 (Table 1) were designed from published mRNA sequences using DS Gene software (Accelrys, San Diego, CA).…”

Expression of CD9 in frozen–thawed mouse oocytes: preliminary experience

“…We described elsewhere a modified reverse transcription (RT) method for mouse oocytes (16). RT-Master Mix was prepared as recommended by the manufacturer (Invitrogen, Carlsbad, CA).…”