Different pattern of T cell receptor ? gene rearrangement in tumour-infiltrating lymphocytes and peripheral blood in patients with solid tumours

Kowalczyk, Dariusz W.; Skorupski, Witold; Drews, M; Nowak, Jerzy

doi:10.1007/s002620050125

Cited by 5 publications

(7 citation statements)

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“…Recent reports demonstrated that γδ T cells play a role in repair of damaged intestinal epithelial cells 5,6) and have the capacity to recognize certain antigens, such as special antigens on the surface of tumor cells and some superantigens, and that their cytotoxic activity is enhanced by TNFα. [15][16][17][18][19] Notably, it was reported that Vδ1-γδ T cells, which are predominant in the human intestinal mucosa, recognized the major histocompatibility antigen-related molecules, MICA and MICB, which are expressed on the surface of intestinal epithelial cells. The expression of the molecules was enhanced in colon tumor cells.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Formation of Azoxymethane‐induced Colorectal Adenocarcinoma in γδ T Lymphocyte‐deficient Mice

Matsuda

Kudoh

Katayama

2001

Japanese Journal of Cancer Research

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γδ T cells have been reported to secrete a cytokine which supports the elimination of impaired epithelial cells and to maintain the normal configuration of the intestinal epithelium. 5,6) However, the precise functions of γδ T cells have not been elucidated. Some recent studies have described the effect of γδ T cells on intestinal tumor cells. γδ T cells regulated the cytotoxic activities of NK cells and CTL, of which the targets were intestinal tumor cells.7) The frequency of tumorinfiltrated γδ T cells was lowered in human well-to-moderately differentiated colorectal adenocarcinoma, 8) suggesting a relationship between γδ T cells and the formation and progression of colorectal adenocarcinoma. Notably, γδ T cells in which the variable region on the TCRδ chain is Vδ1 (Vδ1-γδ T cells) were reported to be predominant in iIEL, to recognize the major histocompatibility complex class I-related molecules A and B (MICA and MICB) and to have cytotoxic activity against intestinal tumor cells. 9)The above report also suggests that γδ T cells in iIEL may affect the formation and progression of colorectal adenocarcinoma.The present study was designed to examine the effect of γδ T cells on the formation and progression of chemically induced colorectal adenocarcinoma using γδ T cell-deficient mice. Azoxymethane (AOM) was administered to wild-type C57BL/6 mice, αβ T cell-deficient mice (TCRα-gene knockout mice) and γδ T cell-deficient mice (TCRδ-gene knockout mice) and the formation of macroscopic tumors and microscopic aberrant crypt foci in colorectal mucosa was examined. The effect of γδ T cells on the formation and progression of colorectal adenocarcinoma is discussed in the light of the results of the present study. MATERIALS AND METHODSMice Homozygous TCRα-gene knockout mice, 10) homozygous TCRδ-gene knockout mice 11) and wild-type C57BL/6 (the background of the knockout mice) were purchased from Jackson Laboratories (Bar Harbor, ME). The mice were bred and maintained in a clean air system (EBAC-S, Clea Japan Inc., Tokyo). Two-to 3-month-old mice (body weight: 15-20 g) were used for experiments. Induction of colorectal tumors and aberrant crypt foci For the induction of colorectal tumors, mice were administered AOM (10 mg/kg weight) intraperitoneally (i.p.) once

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Section: Discussionmentioning

confidence: 99%

Enhanced Formation of Azoxymethane‐induced Colorectal Adenocarcinoma in γδ T Lymphocyte‐deficient Mice

Matsuda

Kudoh

Katayama

2001

Japanese Journal of Cancer Research

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“…The exact role of Vγ1 and Vδ1 TCR + cells in tumour immunology has still to be defined; however, γ/δ TCR + cells may be stimulated to secrete lymphokines and may act as activated killer cells and exert some cytolytic activity on several tumours 1. Extending earlier porcine7 and human6 28 reports on the existence of these cells in melanomas and the occurrence of spontaneous regression of melanomas,11 patient survival was analysed with respect to the infiltration by these cells. Interestingly, a highly significant positive correlation for survival could be defined.…”

Section: Discussionmentioning

confidence: 99%

“…γ and δ TCR + cells have been detected to selectively accumulate or to be amplified in non-ocular solid melanomas and are thought to indicate a unique type of host reaction against the tumour 6. Furthermore, tumour infiltrating cells expressing the γ and δ TCR + CD3 + complex have been found crucial for spontaneous regression of inheritable melanomas in the Sinclair miniature swine 7…”

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confidence: 99%

alpha /beta - and gamma /delta TCR+ lymphocyte infiltration in necrotising choroidal melanomas

Bialasiewicz

Richard

1999

British Journal of Ophthalmology

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T cells can play a central role in the local immune response to cancer with tumour specific T lymphocyte reactivity provided by the TCR variable genes V /V chain heterodimer or the V or V chains. The identification and functional characterisation of MHC class I restricted antigen specific TCR expressing tumour infiltrating lymphocytes (TIL) has been the subject of intense research in the recent years, since tumour reactivity by these genes may provide novel applications for immunotherapy including the redirection of TIL specificity, and bone marrow stem cell therapy.

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“…Preferential expansion and antitumor responses of subsets of γ/δ TILs have been found in many kinds of cancer, including colorectal cancer [4, 5], lung adenocarcinoma [6, 7]and pancreatic cancer [8]. Such γ/δ T cells demonstrated a non–MHC–restricted recognition and cytolytic activity to autologous/allogeneic tumor cell lines.…”

Section: Introductionmentioning

confidence: 99%

Expansion and Immunological Study of Human Tumor Infiltrating Gamma/Delta T Lymphocytes in vitro<sup>1</sup>

Yu¹,

Wang²,

Chen³

et al. 1999

Int Arch Allergy Immunol

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γ/δ T cells have stimulated a lot of interest because of their unique features in antigen recognition and cytotoxicities to many autologous and/or allogeneic tumor cells. We have developed a novel method to selectively expand larger amounts of human tumor–infiltrating γ/δ T lymphocytes (γ/δ TILs) ex vivo by immobilized pan– anti–TCRγ/δ monoclonal antibody in the presence of exogenous IL–2. The expanded γ/δ TILs mainly expressed CD45RO and HLA–DR molecules and did not express CD4. CD8+ γ/δ TILs accounted for 19% of γ/δ TILs. The expression of CD25 molecule on expanded γ/δ T cells was inducible and downregulated following a time course. The Vδ1 and Vδ2 subsets amount to 37 and 58%, respectively. The expanded γ/δ TILs show an IL–2–dependent proliferation, MHC class I–unrestricted and TCRγ/δ–related cytotoxicities to two MHC class I⁺ and two MHC class I⁺ allogeneic tumor cell lines in vitro.

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Different pattern of T cell receptor ? gene rearrangement in tumour-infiltrating lymphocytes and peripheral blood in patients with solid tumours

Cited by 5 publications

References 0 publications

Enhanced Formation of Azoxymethane‐induced Colorectal Adenocarcinoma in γδ T Lymphocyte‐deficient Mice

Enhanced Formation of Azoxymethane‐induced Colorectal Adenocarcinoma in γδ T Lymphocyte‐deficient Mice

alpha /beta - and gamma /delta TCR+ lymphocyte infiltration in necrotising choroidal melanomas

Expansion and Immunological Study of Human Tumor Infiltrating Gamma/Delta T Lymphocytes in vitro<sup>1</sup>

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