Different photosynthetic responses of haploid and diploid Emiliania huxleyi (Prymnesiophyceae) to high light and ultraviolet radiation

Ruan, Zuoxi; Lu, Ming–Wei; Lin, Hongmin; Chen, Shanwen; Li, Ping; Chen, Weizhou; Xu, Hao; Qiu, Dajun

doi:10.1186/s40643-023-00660-5

Cited by 4 publications

(3 citation statements)

References 61 publications

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“…8; Supplementary Text; Dataset S4). The presence of flagella and a likely more mixotrophic nutrition in the haploid are in contrast with more abundant chlorophyll a (37,38) and the up-regulation of photosynthesis-related genes in the diploid. The low abundance of DMSP in the haploid (29) is possibly due to the up-regulation of DMSP catabolic enzymes in both demethylation and cleavage pathways, including two key DMSP lyases, DddD and Alma.…”

Section: Discussionmentioning

confidence: 97%

Haplotype-aware multiomics unveils the regulatory basis of haplodiplontic life-cycle differentiation in a cosmopolitan marine alga

Kao,

Lai,

Wang

et al. 2024

Preprint

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Gephyrocapsa huxleyi, a key coccolithophore alga influencing the global carbon cycle through photosynthesis and calcification, undergoes a haplodiplontic sexual life cycle with a calcifying non-flagellate diploid and a non-calcifying biflagellate haploid stage. To reveal the molecular basis of their morpho-physiological distinctions, we generated chromosome-level genome assemblies for a pair of isogenic haploid and diploid model strains and conducted haplotype-aware analyses of their multiomic features. In addition to calcification and flagella, transcriptomes and proteomes of haploid and diploid cells modulate their differentiation in photosynthesis, sulfatases, DMSP degradation, DNA replication, and endomembrane system and transport. Haploid-diploid differential gene expression can be partially attributable to allelic imbalance (allele-specific expression) in diploid cells. Gene transcript abundance is positively associated with both CG and CHG gene-body DNA methylation, which can be inheritable, allele-specific, and differentiated between life-cycle phases. This multiomic study unravels the regulatory basis of unicellular algal life-cycle differentiation and provides valuable resources for investigating the ecologically important coccolithophore algae.

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Section: Discussionmentioning

confidence: 97%

Haplotype-aware multiomics unveils the regulatory basis of haplodiplontic life-cycle differentiation in a cosmopolitan marine alga

Kao,

Lai,

Wang

et al. 2024

Preprint

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“…huxleyi 58,74 . However, in our experiments, cultures subjected to UV did not exhibit a comparable effect (Figure S5).…”

Section: External Application Of Vitamin D Impacts Algal Traits Simil...mentioning

confidence: 99%

The Role of Vitamin D inEmiliania huxleyi: A Microalgal Perspective on UV Exposure

Eliason,

Malitsky,

Panizel

et al. 2023

Preprint

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An essential interaction between sunlight and eukaryotes involves the production of vitamin D through exposure to ultraviolet-B (UV-B) radiation. While extensively studied in vertebrates, the role of vitamin D in non-animal eukaryotes like microalgae remains unclear. Emiliania huxleyi, a microalga inhabiting shallow ocean depths exposed to UV-B radiation, is well-suited for this research. Our results show that E. huxleyi can produce vitamin D2 and D3, pointing to their potential role in the algal physiology. We further show that E. huxleyi algae respond to vitamin D at the transcriptional level, regulating the expression of protective mechanisms such as the light-harvesting complex stress-related protein (LHCSR) and heme oxygenase, and that vitamin D enhances the algal photosynthetic performance while reducing harmful reactive oxygen species buildup. Understanding the function of vitamin D in E. huxleyi has broader implications, shedding light on its role in non-animal eukaryotes and its potential importance in marine ecosystems. This research sets the stage for further investigations into the complex relationship between sunlight, vitamin D, and microalgal physiology, which contributes to our understanding of how eukaryotes adapt to diverse environmental conditions.

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“…For example, the non-calcified strain exhibited photoinhibition under a moderate level of irradiance of above 400~500 µmol m −2 s −1 [24]. The photosynthesis of the non-calcified haploid strain RCC 1217 exhibited greater sensitivity to high visible light levels compared to the calcified strain PML B92/11, but demonstrated resistance to ultraviolet radiation (UVR)-induced inhibition, demonstrating a capacity to adapt to a specific ecological niche [33]. Previous studies have indicated that cellular organic contents between the two morphotypes may differ, but the results have been inconsistent across experiments.…”

Section: Introductionmentioning

confidence: 99%

Phagocytosis in Marine Coccolithophore Gephyrocapsa huxleyi: Comparison between Calcified and Non-Calcified Strains

Ye,

Wang,

et al. 2024

Biology

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Coccolithophores play a significant role in marine calcium carbonate production and carbon cycles, attributing to their unique feature of producing calcareous plates, coccoliths. Coccolithophores also possess a haplo-diplontic life cycle, presenting distinct morphology types and calcification states. However, differences in nutrient acquisition strategies and mixotrophic behaviors of the two life phases remain unclear. In this study, we conducted a series of phagocytosis experiments of calcified diploid and non-calcified haploid strains of coccolithophore Gephyrocapsa huxleyi under light and dark conditions. The phagocytosis capability of each strain was examined based on characteristic fluorescent signals from ingested beads using flow cytometry and fluorescence microscopy. The results show a significantly higher phagocytosis percentage on fluorescent beads in the bacterial prey surrogates of the non-calcified haploid Gephyrocapsa huxleyi strain, than the calcified diploid strain with or without light. In addition, the non-calcified diploid cells seemingly to presented a much higher phagocytosis percentage in darkness than under light. The differential phagocytosis capacities between the calcified diploid and non-calcified haploid Gephyrocapsa huxleyi strains indicate potential distinct nutritional strategies at different coccolithophore life and calcifying stages, which may further shed light on the potential strategies that coccolithophore possesses in unfavorable environments such as twilight zones and the expanding coccolithophore niches in the natural marine environment under the climate change scenario.

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Different photosynthetic responses of haploid and diploid Emiliania huxleyi (Prymnesiophyceae) to high light and ultraviolet radiation

Cited by 4 publications

References 61 publications

Haplotype-aware multiomics unveils the regulatory basis of haplodiplontic life-cycle differentiation in a cosmopolitan marine alga

Haplotype-aware multiomics unveils the regulatory basis of haplodiplontic life-cycle differentiation in a cosmopolitan marine alga

The Role of Vitamin D inEmiliania huxleyi: A Microalgal Perspective on UV Exposure

Phagocytosis in Marine Coccolithophore Gephyrocapsa huxleyi: Comparison between Calcified and Non-Calcified Strains

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