S u m m a r yBroccoli (Brassica oleracea L. var. italica) contains numerous biologically active compounds. However, their levels change during processing and storage.The objective of the research study was to determine the effect of short-term storage (1 ÷ 4 days) of broccoli heads at different temperatures (3 ºC, 16 ºC, and 21 ºC) on the contents of phenolic acids, selected flavonoids (quercetin and kaempferol), and tocopherols. Broccoli heads were harvested in autumn when they reached their maximum size and their buds were 2 mm in diameter.The content of phenolics and tocopherols depended on the storage time of broccoli heads. The lowest content of phenolics was found immediately after the broccoli was harvested, whereas the highest content thereof was reported after 4 days of storage. However, the storage temperature did not impact the contents of those compounds. In the research study, the content of caffeic acid was reported to be highest in the fresh broccoli heads; the content of sinapic acid was the second-highest, and that of the ferulic acid was the third-highest. The content of kaempferol in the broccoli heads was higher than that of quercetin. No differences were reported in the content of quercetin and kaempferol at particular storage temperatures on every single day of the experiment. An increase was reported only when compared with the control sample (the 0 day of storage). In the broccoli heads, the predominant tocopherol was α-tocopherol. The lowest loss in the total tocopherols occurred while storing at a temperature of 3 ºC and the highest: at a temperature of 21 ºC.Key words: Brassica oleracea L. var. italica, HPLC, phenolics, tocopherols, storage, temperature Dr A. Kałużewicz, dr J. Lisiecka, dr W. Krzesiński, dr T. Spiżewski, dr B. Frąszczak, Katedra Warzywnictwa, Wydz. Ogrodnictwa i Architektury, Uniwersytet Przyrodniczy w Poznaniu, ul. Dąbrowskiego 159, dr M. Gąsecka, dr hab. A. Waśkiewicz, Katedra Chemii, Wydz. Technologii Drewna, Uniwersytet Przyrodniczy w Poznaniu, ul. Wojska Polskiego 25,
128Alina Kałużewicz i wsp.
IntroductionBroccoli (Brassica oleracea L. var. italica) is a vegetable of high nutritional value owing to the abundance of health-promoting phytochemicals therein [1]. The variation among the contents of bioactive components therein depends both on the genetics and the environment including the conditions of growing it and the pre-harvest and/or postharvest conditions [25]. Of all the bioactive compounds, phenolics deserve special attention [1] because of their role in preventing cancer and cardiovascular diseases [3]. Owing to their health-promoting effects, the phenolic compounds were intensively investigated over recent years [29]. Depending on their structure, phenolic compounds may be classified into simple phenols, phenolic acids, hydroxycinnamic acid derivatives, and flavonoids [10].Phenolic compounds, especially flavonoids, are among the major antioxidants in Brassica vegetables [24]. Many research studies show that broccoli has a very high content of total pheno...