Different pre-treatments and kinetic models for bioethanol production from lignocellulosic biomass: A review

Pendse, Dhanashri S.; Deshmukh, Minal; Pande, Ashwini

doi:10.1016/j.heliyon.2023.e16604

Cited by 20 publications

(8 citation statements)

References 122 publications

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“…Reduction of particle size in micrometers assists in greater pre-treatment and enzymatic hydrolysis efficiency. The mechanical grinding process increases the surface area of the matter and reduces the crystallinity of the cellulose, providing greater efficiency in the removal of lignin with pre-treatment with dilute acid and greater absorption of enzymes in the hydrolysis treatment to release glucose (DEVI, et al, 2021;PENDSE;DESHMUKH;PANDE, 2023).…”

Section: Resultsmentioning

confidence: 99%

Pre-Treatment With Hydrochloric Acid and Enzymatic Hydrolysis of Sugar Cane Bagass to Obtain Glucose

Abreu,

Silva

2023

JER

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Pretreatment and enzymatic hydrolysis are two important steps within the process of converting lignocellulosic biomass into glucose. In this work, sugarcane bagasse (BCA) ground into particles smaller than 40 mesh (< 425 µm) was pre-treated with hydrochloric acid (HCl) in different concentrations (0.75, 1.0 and 1.25 mol/L), in time varying from 30 to 90 min by autoclaving (121 ºC / 1 atm) in solid ratio :net 1:10. The pretreated BCA was subjected to hydrolysis with commercial enzymes at a solid:liquid ratio of 1:30, with100 µL of cellulase mixtures and 100 µL of Aspergilus niger laccase, in citrate-phosphate buffer (50 mM; pH 4.8) for 72h at 50 ºC under agitation at 150 rpm. Pre-treatment with HCl showed an efficiency in delignification of BCA of 59.2% and the glycan fraction increased to 64.48% in the solid residue of BCA recovered after pretreatment carried out with HCl 1.25 mol/L, time 30 min. Within these operational parameters, enzymatic hydrolysis continued for 72h. Within a mass balance analysis, a solids recovery of 42.8% was observed for the pre-treatment stage with HCl. Therefore, for every 100 g of pre-treated BCA, within the process, residue with 64.48 g of glycan and 9.23 g of lignin was recovered and for every 100 g of BCA recovered after pre-treatment and enzymatically treated with cellulases and laccase it was possible to obtain 12.0g of glycan hydrolyzed into glucose. It is concluded that pre-treatment with dilute acid and hydrolysis with commercial enzymes showed efficiency in converting BCA glycan into glucose.

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Section: Resultsmentioning

confidence: 99%

Pre-Treatment With Hydrochloric Acid and Enzymatic Hydrolysis of Sugar Cane Bagass to Obtain Glucose

Abreu,

Silva

2023

JER

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“…Yeast cells were cultivated in YP with 2% cellobiose medium to prepare inoculums for fermentation and cells were harvested by centrifugation (4000 rpm, 5 min) at mid-exponential phase. Flask fermentation experiments were performed in 40 ml YNB medium containing 10% cellobiose; 8% cellobiose and 4% xylose; 5% cellobiose, 4% xylose, and 5% glucose (Carroll and Somerville 2009 , Pendse et al 2023 ) using 100 ml shaking flask under oxygen limited conditions (45°C, 140 rpm). The initial biomass concentration for fermentation experiments was 2 g (dry weight)/l.…”

Section: Methodsmentioning

confidence: 99%

Engineering of Ogataea polymorpha strains with ability for high-temperature alcoholic fermentation of cellobiose

Vasylyshyn,

Dmytruk,

Sybirnyy

et al. 2024

FEMS Yeast Research

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Successful conversion of cellulosic biomass into biofuels requires organisms capable of efficiently utilizing xylose as well as cellodextrins and glucose. Ogataea (Hansenula) polymorpha is the natural xylose-metabolizing organism and is one of the most thermotolerant yeasts known, with a maximum growth temperature above 50°C. Cellobiose-fermenting strains, derivatives of an improved ethanol producer from xylose O. polymorpha BEP/cat8∆, were constructed in this work by the introduction of heterologous genes encoding cellodextrin transporters (CDTs) and intracellular enzymes (β-glucosidase or cellobiose phosphorylase) that hydrolyze cellobiose. For this purpose, the genes gh1-1 of β-glucosidase, CDT-1 m and CDT-2 m of cellodextrin transporters from Neurospora crassa and the CBP gene coding for cellobiose phosphorylase from Saccharophagus degradans, were successfully expressed in O. polymorpha. Through metabolic engineering and mutagenesis, strains BEP/cat8∆/gh1-1/CDT-1 m and BEP/cat8∆/CBP-1/CDT-2mAM were developed, showing improved parameters for high-temperature alcoholic fermentation of cellobiose. The study highlights the need for further optimization to enhance ethanol yields and elucidate cellobiose metabolism intricacies in O. polymorpha yeast. This is the first report of the successful development of stable methylotrophic thermotolerant strains of O. polymorpha capable of co-utilizing cellobiose, glucose, and xylose under high-temperature alcoholic fermentation conditions at 45°C.

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“…Typical LCB comprise cellulose (35-55%), hemicellulose (20-40%), and lignin (10-25%) as the main components along with trace proteins, pectin, ash, and other substances, such as inorganic materials, waxes, fats, and phenolics [43][44][45][46]. However, the composition varies among plant species owing to factors such as age, harvesting season, and cultivation conditions.…”

Section: Lignocellulosic Biomass Compositionmentioning

confidence: 99%

Comparative Analysis of Acidic and Alkaline Pretreatment Techniques for Bioethanol Production from Perennial Grasses

Johannes,

Xuan

2024

Energies

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This review paper examines acid and alkaline pretreatments on perennial grasses for second-generation (2G) bioethanol production, a relatively unexplored area in this field. It compares the efficiency of these pretreatments in producing fermentable sugar and bioethanol yield. This study finds that alkaline pretreatment is more effective than acidic pretreatment in removing lignin and increasing sugar yield, leading to higher ethanol yields. However, it is costlier and requires longer reaction times than acidic pretreatment, while acidic pretreatment often leads to the formation of inhibitory compounds at higher temperatures, which is undesirable. The economic and environmental impacts of lignocellulosic biomass (LCB) are also assessed. It is revealed that LCB has a lower carbon but higher water footprint and significant costs due to pretreatment compared to first-generation biofuels. This review further explores artificial intelligence (AI) and advanced technologies in optimizing bioethanol production and identified the gap in literature regarding their application to pretreatment of perennial grasses. This review concludes that although perennial grasses hold promise for 2G bioethanol, the high costs and environmental challenges associated with LCB necessitate further research. This research should focus on integrating AI to optimize the pretreatment of LCB, thereby improving efficiency and sustainability in 2G biofuel production.

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Different pre-treatments and kinetic models for bioethanol production from lignocellulosic biomass: A review

Cited by 20 publications

References 122 publications

Pre-Treatment With Hydrochloric Acid and Enzymatic Hydrolysis of Sugar Cane Bagass to Obtain Glucose

Pre-Treatment With Hydrochloric Acid and Enzymatic Hydrolysis of Sugar Cane Bagass to Obtain Glucose

Engineering of Ogataea polymorpha strains with ability for high-temperature alcoholic fermentation of cellobiose

Comparative Analysis of Acidic and Alkaline Pretreatment Techniques for Bioethanol Production from Perennial Grasses

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