Different process configurations for bioethanol production from pretreated olive pruning biomass

Manzanares, Paloma; Negro, M.J.; Oliva, J.; Saéz, Felicia; Ballesteros, Ignacio; Ballesteros, Mercedes; Cara, Cristóbal; Castro, Eulógio; Ruiz, Encarnación

doi:10.1002/jctb.2604

Cited by 80 publications

(40 citation statements)

References 35 publications

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“…The enzymatic presaccharification yield decreased with increasing substrate loadings (constant enzyme to substrate ratio), obtaining the lowest yield value (53.7%) at 25% (w/w) (Table 1). These results are in line with others reported on different steam‐exploded lignocellulosic substrates 34–36. The mechanism behind the decreasing conversion has been attributed to different factors, including end‐product inhibition or inhibition by degradation products, changes in substrate reactivity during enzymatic hydrolysis, mass transfer limitations, and unproductive adsorption of enzymes 14, 33.…”

Section: Resultssupporting

confidence: 91%

“…Enzymatic presaccharification of WIS with the standard NS50013/NS50010 enzyme mixture resulted in higher glucose concentrations as the substrate loadings increased (Table 1), obtaining the highest glucose concentration (100.5 g L −1 ) at 25% (w/w). In all the loadings assayed, the major degree of glucan conversion was achieved within the first 24 h of the enzymatic presaccharification (Table 1), in accordance with previous studies 33, 34. Only at a substrate loading of 25% (w/w), an increase of glucose production was observed from 24 to 48 h of hydrolysis (Table 1).…”

Section: Resultssupporting

confidence: 90%

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Tunable Epoxidation of Single‐Walled Carbon Nanotubes by Isolated Methyl(trifluoromethyl)dioxirane

Annese¹,

D’Accolti²,

Giambastiani³

et al. 2014

Eur J Org Chem

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Organic functionalization of carbon nanotube sidewalls is a tool of primary importance in material science and nanotechnology, equally from a fundamental and an applicative point of view. In this paper, a mild and easily tunable approach to the sidewall decoration of single-walled carbon nanotubes (SWCNTs) with epoxides and their subsequent derivatization (ring opening) upon treatment with reactive nucleophiles is presented. The treatment of HiPco purified SWCNTs with dioxirane solutions results in highly oxidized CNTs, which are used as electrophilic platforms for their successive derivatization/functionalization. As a result of the choice of accessible, easy to handle and store dioxiranes, multiple oxidation cycles could be performed on the same sample, thus allowing for a final improvement in the extent of oxidation at the CNT sidewall

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 90%

Tunable Epoxidation of Single‐Walled Carbon Nanotubes by Isolated Methyl(trifluoromethyl)dioxirane

Annese¹,

D’Accolti²,

Giambastiani³

et al. 2014

Eur J Org Chem

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“…Same enhancement in ethanol production efficiency was also demonstrated by Kim et al [25], who observed an increase in ethanol yield from 0.31 g/g to 0.43 g/g total solid when applying a Separate Hydrolysis and Fermentation (SHF) process instead of Simultaneous Saccharification and Fermantation (SSF) process on cafeteria food waste. Manzanares et al [29] also found that with increasing initial DM content, a separate saccharification step improves fermentation of liquid hot waterpretreated olive-pruning biomass. Finally, Hoyer et al [30] reported that with increasing DM content, even 4 h of saccharification could significantly improve fermentation of softwoods.…”

Section: Resultsmentioning

confidence: 96%

Utilization Of Household Food Waste For The Production Of Ethanol At High Dry Material Content

Μάτσακας¹,

Kekos²,

Loizidou³

et al. 2015

Solid Waste as a Renewable Resource

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Background: Environmental issues and shortage of fossil fuels have turned the public interest to the utilization of renewable, environmentally friendly fuels, such as ethanol. In order to minimize the competition between fuels and food production, researchers are focusing their efforts to the utilization of wastes and by-products as raw materials for the production of ethanol. household food wastes are being produced in great quantities in European Union and their handling can be a challenge. Moreover, their disposal can cause severe environmental issues (for example emission of greenhouse gasses). On the other hand, they contain significant amounts of sugars (both soluble and insoluble) and they can be used as raw material for the production of ethanol. Results: Household food wastes were utilized as raw material for the production of ethanol at high dry material consistencies. A distinct liquefaction/saccharification step has been included to the process, which rapidly reduced the viscosity of the high solid content substrate, resulting in better mixing of the fermenting microorganism. This step had a positive effect in both ethanol production and productivity, leading to a significant increase in both values, which was up to 40.81% and 4.46 fold, respectively. Remaining solids (residue) after fermentation at 45% w/v dry material (which contained also the unhydrolyzed fraction of cellulose), were subjected to a hydrothermal pretreatment in order to be utilized as raw material for a subsequent ethanol fermentation. This led to an increase of 13.16% in the ethanol production levels achieving a final ethanol yield of 107.58 g/kg dry material. Conclusions: In conclusion, the ability of utilizing household food waste for the production of ethanol at elevated dry material content has been demonstrated. A separate liquefaction/saccharification process can increase both ethanol production and productivity. Finally, subsequent fermentation of the remaining solids could lead to an increase of the overall ethanol production yield.

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“…The highest ethanol concentration at 72 h SSF were 68.28 g/L (A) and 78.25 g/L (B).According to literature, the threshold of economic profitability corresponds to bioethanol concentrations in the fermentation broth in the range 4-5 volume percent. Achieving this threshold entails the utilization of media containing 15-20% solids (on dry basis) [28]. Therefore, increasing substrate loading required to produce high-concentration ethanol so that the process is more profitable.…”

Section: Effect Of Variation Of Substrate Concentration Loading Direcmentioning

confidence: 99%

The Effect of Substrate Loading On Simultaneous Saccharification And Fermentation Process For Bioethanol Production from Oil Palm Empty Fruit Bunches

et al. 2015

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Increasing energy demand and concern about increased greenhouse gas emissions make lignocellulosic biomass increasingly to be recognized as having great potential for biofuel and biomaterial production based on the biorefinery concept. Oil Palm Empty Fruit Bunches (EFBs) is one of the major solid wastes in the palm oil industries as a source of lignocellulosic biomass. Cellulose is the highest component of EFBs that can be converted to ethanol. The aim of this research was to investigate the different strategies for high substrate loading on SSF process of bioethanol production from EFBs. Increasing substrate loading is one of the most important challenges to make bioethanol production more economical. This research used two methods to increase the substrate concentration loading on Simultaneous Saccharification and Fermentation (SSF) i.e: direct variation of substrate concentration loading and substrate loading gradually to obtain a high-concentration substrate. A range of substrate loading was from 15% to 25% (g.mL -1 ). The SSF process was carried out at 32 o C, pH 4.8, and 150 rpm for 72 hours. The result shows that the highest concentration of ethanol can be produced by a high concentration of substrate loading gradually. The highest ethanol concentration was 83.40 g.L -1 (80.21% ethanol yield) by using 25% (g.mL -1 )substrate loading gradually, 18 FPU/g substrate enzyme Cellic® Ctec2 and 20% Cellic® Htec2 (based on volume of Cellic® Ctec2), and 1% (g.mL -1 ) yeast Saccharomyces cereviceae in SSF process. Whereas, 20% (g.mL -1 ) concentration substrate loading by directly or gradually produce almost same ethanol concentration.

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Different process configurations for bioethanol production from pretreated olive pruning biomass

Cited by 80 publications

References 35 publications

Tunable Epoxidation of Single‐Walled Carbon Nanotubes by Isolated Methyl(trifluoromethyl)dioxirane

Tunable Epoxidation of Single‐Walled Carbon Nanotubes by Isolated Methyl(trifluoromethyl)dioxirane

Utilization Of Household Food Waste For The Production Of Ethanol At High Dry Material Content

The Effect of Substrate Loading On Simultaneous Saccharification And Fermentation Process For Bioethanol Production from Oil Palm Empty Fruit Bunches

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