Different proteomic protein patterns in saliva of Sjögren's syndrome patients

Fleissig, Yoram; Deutsch, Omer; Reichenberg, E.; Redlich, Meir; Zaks, Batia; Palmon, Aaron; Aframian, Doron J.

doi:10.1111/j.1601-0825.2008.01465.x

Cited by 81 publications

(63 citation statements)

References 27 publications

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“…Saliva protein composition changes have been observed in SS; however, variation among patients did not correlate with clinical, serological, or histological disease severity (48). The removal of high molecular weight proteins leads to a near complete fall in saliva lubrication (49), suggesting the high molecular weight mucins are important for oral lubrication.…”

Section: Discussionmentioning

confidence: 99%

Reduced Mucin-7 (Muc7) Sialylation and Altered Saliva Rheology in Sjögren's Syndrome Associated Oral Dryness

Chaudhury

Proctor

Karlsson

et al. 2016

Molecular & Cellular Proteomics

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Sjö gren's syndrome is a chronic autoimmune disorder characterized by lymphocytic infiltration and hypofunction of salivary and lacrimal glands. This loss of salivary function leads to oral dryness, impaired swallowing and speech, and increased infection and is associated with other autoimmune diseases and an increased risk of certain cancers. Despite the implications of this prevalent disease, diagnosis currently takes years, partly due to the diversity in patient presentation. Saliva is a complicated biological fluid with major constituents, including heavily glycosylated mucins MUC5B and MUC7, important for its viscoelastic and hydrating and lubricating properties. This study investigated Sjö gren's patient's perception of dryness (bother index questionnaires) along with the rheological, protein composition, and glycan analysis of whole mouth saliva and the saliva on the mucosal surface (residual mucosal saliva) to understand the properties that most affect patient wellbeing. Sjö gren's patients exhibited a statistically significant reduction in residual mucosal saliva, salivary flow rate, and extensional rheology, spinnbarkeit (stringiness). Although the concentration of mucins MUC5B and MUC7 were similar between patients and controls, a comparison of protein Western blotting and glycan staining identified a reduction in mucin glycosylation in Sjö gren's, particularly on MUC7. LC-MS/MS analysis of O-glycans released from MUC7 by ␤-elimination revealed that although patients had an increase in core 1 sulfation, the even larger reduction in sialylation resulted in a global decline of charged glycans. This was primarily due to the loss of the extended core 2 disialylated structure, with and without fucosylation. A decrease in the extended, fucosylated core 2 disialylated structure on MUC7, residual mucosal wetness, and whole mouth saliva flow rate appeared to have a negative and cumulative effect on the perception of oral dryness. The observed changes in MUC7 glycosylation could be a potential diagnostic tool for saliva quality and taken into consideration for future therapies for this multifactorial syndrome. Molecular & Cellular

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Section: Discussionmentioning

confidence: 99%

Reduced Mucin-7 (Muc7) Sialylation and Altered Saliva Rheology in Sjögren's Syndrome Associated Oral Dryness

Chaudhury

Proctor

Karlsson

et al. 2016

Molecular & Cellular Proteomics

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“…There are three major methods of high-abundance protein removal 164 : enzyme-substrate absorption method used for alpha-amylase affinity removal, 165 immunodepletion method, and combinatorial peptide ligand library (CPLL). 166 Proteomic analysis of saliva is commonly used in the diagnostics of oral diseases as well as general health disorders such as oral candidiasis, 167 OSCC, 168 glossodynia, 169 head and neck squamous cell cancer, 170 Sjö gren's syndrome 171 HIV, 167 autism, 172 fibromyalgia, 173 breast cancer, 174 lung cancer, melanoma, 82 or pancreatic cancer. 26 …”

Section: Proteomicsmentioning

confidence: 99%

Saliva diagnostics – Current views and directions

Kaczor‐Urbanowicz

Carreras‐Presas

Aro

et al. 2016

Exp Biol Med (Maywood)

358

271

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The purpose of this mini-review is to make an update about the present and future applications of saliva as a diagnostic biofluid in many fields of science such as dentistry, medicine and pharmacotherapy. Using saliva as a fluid for diagnostic purposes would be a huge breakthrough for both patients and healthcare providers since saliva collection is easy, non-invasive and inexpensive. We will go through the current main diagnostic applications of saliva, and provide a highlight on the emerging, newly developing technologies and tools for cancer screening, detection and monitoring. AbstractIn this review, we provide an update on the current and future applications of saliva for diagnostic purposes. There are many advantages of using saliva as a biofluid. Its collection is fast, easy, inexpensive, and non-invasive. In addition, saliva, as a ''mirror of the body,'' can reflect the physiological and pathological state of the body. Therefore, it serves as a diagnostic and monitoring tool in many fields of science such as medicine, dentistry, and pharmacotherapy. Introduced in 2008, the term ''Salivaomics'' aimed to highlight the rapid development of knowledge about various ''omics'' constituents of saliva, including: proteome, transcriptome, micro-RNA, metabolome, and microbiome. In the last few years, researchers have developed new technologies and validated a wide range of salivary biomarkers that will soon make the use of saliva a clinical reality. However, a great need still exists for convenient and accurate point-of-care devices that can serve as a non-invasive diagnostic tool. In addition, there is an urgent need to decipher the scientific rationale and mechanisms that convey systemic diseases to saliva. Another promising technology called liquid biopsy enables detection of circulating tumor cells (CTCs) and fragments of tumor DNA in saliva, thus enabling non-invasive early detection of various cancers. The newly developed technology-electric field-induced release and measurement (EFIRM) provides near perfect detection of actionable mutations in lung cancer patients. These recent advances widened the salivary diagnostic approach from the oral cavity to the whole physiological system, and thus point towards a promising future of salivary diagnostics for personalized individual medicine applications including clinical decisions and post-treatment outcome predictions.

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“…Comprehensive analysis and identification of the proteomic contents in human whole and ductal saliva is a necessary first step toward the discovery of saliva protein markers for human disease detection in particular for oral cancer and Sjogren's syndrome. [20,21] Proteomics in periodontal ligament fibroblasts:…”

Section: Proteomics and Dentistrymentioning

confidence: 99%