Different requirements for GATA factors in cardiogenesis are mediated by non‐canonical Wnt signaling

Afouda, Boni A.; Hoppler, Stefan

doi:10.1002/dvdy.22570

Cited by 19 publications

(44 citation statements)

References 50 publications

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“…During organ development, the relative dominance of canonical or non-canonical Wnt pathways often switches in a pattern that correlates temporally with distinct phases of progenitor proliferation and differentiation, respectively (Tian et al, 2010). In addition GATA factors play a central role in regulating this switch from canonical to non-canonical Wnt pathway activation in different organs, including the lung and heart (Afouda and Hoppler, 2011; Afouda et al, 2008; Zhang et al, 2008). Such a stage specific and biphasic role for Wnt signaling has been suggested during hematopoiesis based on analysis of in vitro differentiated embryonic stem cells (Naito et al, 2006; Tarafdar et al, 2013; Vijayaragavan et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

GATA2 regulates Wnt signaling to promote primitive red blood cell fate

Mimoto

Kwon

Green

et al. 2015

Developmental Biology

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Primitive erythropoiesis is regulated in a non cell-autonomous fashion across evolution from frogs to mammals. In Xenopus laevis, signals from the overlying ectoderm are required to induce the mesoderm to adopt an erythroid fate. Previous studies in our lab identified the transcription factor GATA2 as a key regulator of this ectodermal signal. To identify GATA2 target genes in the ectoderm required for red blood cell formation in the mesoderm, we used microarray analysis to compare gene expression in ectoderm from GATA2 depleted and wild type embryos. Our analysis identified components of the non-canonical and canonical Wnt pathways as being reciprocally up- and down-regulated downstream of GATA2 in both mesoderm and ectoderm. We show that up-regulation of canonical Wnt signaling during gastrulation blocks commitment to a hematopoietic fate while down-regulation of non-canonical Wnt signaling impairs erythroid differentiation. Our results are consistent with a model in which GATA2 contributes to inhibition of canonical Wnt signaling, thereby permitting progenitors to exit the cell cycle and commit to a hematopoietic fate. Subsequently, activation of non-canonical Wnt signaling plays a later role in enabling these progenitors to differentiate as mature red blood cells.

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Section: Resultsmentioning

confidence: 99%

GATA2 regulates Wnt signaling to promote primitive red blood cell fate

Mimoto

Kwon

Green

et al. 2015

Developmental Biology

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“…Myc‐tagged Nkx2.5 ( myc‐nkx2.5 / pcs2 and nkx2.5‐myc/pcs2 ) was constructed by the polymerase chain reaction (PCR)‐based strategy from nkx2.5 / pgem3z (supplied by Dr P. Krieg). GATA4 ( gata4gr/psp64t ) and Dickkopf‐1 ( dkk1 / pcs2 ) were gifts from Dr S. Hoppler (Afouda & Hoppler ) and Dr C. Niehrs (Glinka et al . ), respectively.…”

Section: Methodsmentioning

confidence: 99%

Nkx2.5 is involved in myeloid cell differentiation at anterior ventral blood islands in the Xenopus embryo

Sakata

Maéno

2014

Dev Growth Differ

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We have shown previously that two populations of myeloid cells emerge in the anterior and posterior ventral blood islands (aVBI and pVBI) at the different stages in Xenopus laevis embryo. In order to elucidate the regulatory mechanism of myeloid cell differentiation in the aVBI, we examined the role of Nkx2.5, an essential transcription factor for heart differentiation, in regulation of the myeloid cell differentiation in this region. Knockdown of endogenous Nkx2.5 by introducing MO into the dorsal marginal zone (DMZ) suppressed the expression of MHCa as well as that of mpo and spib in the resultant embryos and in DMZ explants made from the injected embryos. Expression of c/ebpa was less affected in the embryos injected with Nkx2.5 MO. The effect of Nkx2.5 MO in myeloid cell differentiation was recovered by coinjection of nkx2.5 or c/ebpa mRNA, indicating that Nkx2.5 functions at the same or the upper level of C/EBPa for the specification of myeloid cells. An attempt to identify transcription factors for myeloid cell differentiation in ventral marginal zone (VMZ) explants demonstrated that coinjection of two transcription factors out of three factors, namely C/EBPa, Nkx2.5 and GATA4, was sufficient to induce a certain amount of mpo expression. We suggest that C/EBPa is an unequivocal factor for myeloid cell differentiation in the aVBI and that Nkx2.5 and GATA4 cooperate with C/EBPa for promotion of myeloid cell differentiation.

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“…In addition to the presence of mesenchymal markers, such as vimentin, SMA, and prolyl-4-hydroxylase, neuroectodermal and endodermal markers have been revealed, confirming that these cells possess differentiation potential towards these lineages. Indeed, MSCs were positive to GATA-4, a transcription factor involved in cardiomyocyte differentiation [86], and connexin-43, highly expressed in cardiomyocytes [87]. When MSCs were induced to cardiomyocyte differentiation, changes in cell morphology with formation of myotube structures were observed [4].…”

Section: Mscs From Whole Umbilical Cordmentioning

confidence: 99%

Phenotype and Differentiation Potential of Stromal Populations Obtained from Various Zones of Human Umbilical Cord: An Overview

Conconi

Liddo²,

Tommasini³

et al. 2011

TOTERMJ

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Fibroblast-like cells with properties similar to mesenchymal stromal cells (MSCs) are present in human umbilical cord (hUC). In accordance with the international minimal criteria for defining multipotent mesenchymal stromal cells, hUC cells are designed MSCs being plastic adherent, positive to specific non hematopoietic lineage biomarkers, able to be both in vitro long term cultured and differentiated into osteoblasts, chondroblasts and adipocytes. In this review, a panoramic view of phenotypic characteristics of hUC cells derived from various UC parts are described. The high heterogeneity of extraction, culture and analysis procedures hinder the ability to precisely identify UC stromal cells. As a result, different phenotypic profiles are detectable not only among the cells obtained from the various parts of cord, but also inside the same UC regions, suggesting that UC-MSCs may represent an unique cell family whose components present various degree of stemness. However, in vitro and in vivo evidence indicates Wharton's jelly as the best source of MSCs, because its cells present a wide range of potential therapeutic applications.

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Different requirements for GATA factors in cardiogenesis are mediated by non‐canonical Wnt signaling

Cited by 19 publications

References 50 publications

GATA2 regulates Wnt signaling to promote primitive red blood cell fate

GATA2 regulates Wnt signaling to promote primitive red blood cell fate

Nkx2.5 is involved in myeloid cell differentiation at anterior ventral blood islands in the Xenopus embryo

Phenotype and Differentiation Potential of Stromal Populations Obtained from Various Zones of Human Umbilical Cord: An Overview

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