Different resistance potential to reovirus in grass carp (Ctenopharyngodon idella) populations and their immune characteristics

Wang, Hongquan; Yang, Hong Qi; Li, Rui; Li, Yaoguo; Xiao, Tiaoyi

doi:10.1007/s10499-020-00623-z

Cited by 4 publications

(8 citation statements)

References 18 publications

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“…However, Xu et al (2016) reported that the C5 gene was downregulated in spleen when the grass carp was infected by GCRV. In addition, Wang et al (2021) reported that the expression of complement C5 was different in different grass carp populations. These results implied that the expression of complement C5 could be influenced by genotype of grass carp.…”

Section: Discussionmentioning

confidence: 99%

Cloning of the full-length cDNA of the gene encoding complement C5 from grass carp (Ctenopharyngodon idella) and its expression in different tissues by following grass carp reovirus infection

Xiao

et al. 2021

Aquacult Int

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Grass carp (Ctenopharyngodon idella) is the most widely produced freshwater aquaculture product worldwide. However, its susceptibility to microbial infections during its cultivation restricts its development. The complement system plays an important role in immune defence. To investigate the transcription of the complement factor C5 and production of its cleavage product C5a in grass carp infected with grass carp reovirus (GCRV), the full-length C5 gene sequence was cloned and sequenced using the rapid amplification of cDNA ends method, and C5 transcription and C5a production in different grass carp tissues at different GCRV infection stages were determined by RT-qPCR and western blotting, respectively. Our results showed that the full-length cDNA of the C5 gene was 5365-bp long, encoding 1687 amino acids. Signal peptide prediction showed that C5 had a secretory signal peptide. After artificial infection with GCRV, C5 transcription in grass carp liver increased significantly before the appearance of symptoms. C5a generation in the liver increased significantly from the onset of disease symptoms, and continued to increase until recovery. GCRV induced C5 cleavage in grass carp, with C5a production.

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Section: Discussionmentioning

confidence: 99%

Cloning of the full-length cDNA of the gene encoding complement C5 from grass carp (Ctenopharyngodon idella) and its expression in different tissues by following grass carp reovirus infection

Xiao

et al. 2021

Aquacult Int

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“…P1 and F1 fish were obtained as previously described [2] and farmed at the Wulong Fishery Base, Liuyang, China (28.30 • N, 113.44 • E). A total of 150 of each of P1 and F1 fish with an average weight of 21.0 ± 5.2 g were transferred to the Aquaculture Base of Hunan Agricultural University for temporary culture two weeks before the experiment [3]. The GCRV918 strain was donated by Lingbing Zeng of the Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences.…”

Section: Fish and Virus Sourcesmentioning

confidence: 99%

“…Total RNA from each sample (approximately 30 mg) was extracted using the RNApure rapid total RNA extraction kit (BioTeke, Beijing, China) and cDNA was synthesized using the ReverAid TM first strand cDNA synthesis kit (Promega, Beijing, China) as previously described [3].…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

“…In 2018, the global production of cultured grass carp was 5,704,000 tons, accounting for 10.5% of the total output of cultured fish worldwide [1]. However, this industry has been seriously hampered because of the development of severe hemorrhagic diseases in this species caused by the Grass Carp Reovirus (GCRV) [2,3]. GCRV infection causes a mortality rate of up to 85% during the summer months [4], resulting in annual economic losses of at least one billion US dollars in China [5].…”

Section: Introductionmentioning

confidence: 99%

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Cloning of Grass Carp Chemokine XC Receptor 1 (XCR1) Gene and Evaluation of Its Expression in Various Organs after GCRV Infection

Liu

Xiao

et al. 2022

Fishes

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Interaction between the chemokine receptor XCR1 and its ligand is closely related to the immune function in animals; however, there are only a few reports on role of XCR1 in the immune system of fish. We aimed to analyze the expression of XCR1 in various organs or tissues of grass carp before and after Grass Carp Reovirus (GCRV) infection to better understand the function of XCR1 in resistance to GCRV infection. We cloned and sequenced the cDNA of grass carp XCR1 and analyzed the molecular structure of XCR1 based its amino acid sequence. Further, we analyzed the relative expression levels of XCR1 in different organs or tissues of male parent grass carp with GCRV resistance (P1) and their first-generation offspring (F1) before and after GCRV infection. Our results show that the total length of cDNA of the grass carp XCR1 gene is 1659 bp and encodes 365 amino acids. XCR1 contains seven conserved transmembrane helical domains. The homologous tertiary structure of XCR1 is similar to its homologs in other species. After artificial GCRV infection, there were significant differences in the expression of the grass carp XCR1 gene in different tissues, at different time points, and between P1 and F1 fish. These results will contribute to our understanding of the role of XCR1 in fish immune responses and contribute to the development of GCRV-resistant grass carp.

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“…These findings suggest that the differences in PRR expression patterns between grass carp and barbel chub might be a reason for the variance in their disease resistance levels. Moreover, complement gene expression is significantly higher in barbel chub than in grass carp [15]. Furthermore, transcriptome analysis of grass carp spleen shows that the expression of various complement components was downregulation after GCRV infection [16].…”

Section: Introductionmentioning

confidence: 98%

Comparative Transcriptomics Analysis Reveals Unique Immune Response to Grass Carp Reovirus Infection in Barbel Chub (Squaliobarbus curriculus)

Huang,

Wang,

et al. 2024

Biology

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Grass carp (Ctenopharyngodon idella) and barbel chub (Squaliobarbus curriculus)—both Leuciscinae subfamily species—demonstrate differences in grass carp reovirus (GCRV) infection resistance. We infected barbel chubs with type II GCRV and subjected their liver, spleen, head kidney, and trunk kidney samples to investigate anti-GCRV immune mechanisms via RNA sequencing and quantitative real-time polymerase chain reaction (qRT-PCR). We identified 139, 970, 867, and 2374 differentially expressed genes (DEGs) in the liver, spleen, head kidney, and trunk kidney, respectively. Across all four tissues, gene ontology analysis revealed significant immune response-related DEG enrichment, and the Kyoto Encyclopedia of Genes and Genomes analysis revealed pattern recognition receptor (PRR) and cytokine-related pathway enrichment. We noted autophagy pathway enrichment in the spleen, head kidney, and trunk kidney; apoptosis pathway enrichment in the spleen and trunk kidney; and complement- and coagulation-cascade pathway enrichment in only the spleen. Comparative transcriptome analysis between GCRV-infected barbel chubs and uninfected barbel chubs comprehensively revealed that PRR, cytokine-related, complement- and coagulation-cascade, apoptosis, and autophagy pathways are potential key factors influencing barbel chub resistance to GCRV infection. qRT-PCR validation of 11 immune-related DEGs confirmed our RNA-seq data’s accuracy. These findings provide a theoretical foundation and empirical evidence for the understanding of GCRV infection resistance in barbel chub and hybrid grass carp–barbel chub breeding.

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Different resistance potential to reovirus in grass carp (Ctenopharyngodon idella) populations and their immune characteristics

Cited by 4 publications

References 18 publications

Cloning of the full-length cDNA of the gene encoding complement C5 from grass carp (Ctenopharyngodon idella) and its expression in different tissues by following grass carp reovirus infection

Cloning of the full-length cDNA of the gene encoding complement C5 from grass carp (Ctenopharyngodon idella) and its expression in different tissues by following grass carp reovirus infection

Cloning of Grass Carp Chemokine XC Receptor 1 (XCR1) Gene and Evaluation of Its Expression in Various Organs after GCRV Infection

Comparative Transcriptomics Analysis Reveals Unique Immune Response to Grass Carp Reovirus Infection in Barbel Chub (Squaliobarbus curriculus)

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