Different response of rat Kupffer cells to lipopolysaccharide after exposure to T-helper cytokines

Atsukawa, Kazuhiro; Saito, Hidetsugu; Takahashi, Masahīko; Akiba, Yasutada; Ohishi, Tazuko; Ebinuma, Hirotoshi; Kurose, Iwao; Ishii, Hiromasa

doi:10.1016/s0928-4346(97)00365-4

Cited by 2 publications

(1 citation statement)

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“…(17,18) Our previous studies (19,20) demonstrating the differential effects of Th1 and Th2 cytokines on hepatic inflammation through hepatic residual macrophage Kupffer cells also support that the Th1/Th2 balance plays a crucial role in chronic hepatitis. Thus, genetically determined and differentially expressed IRF-1, which is important to the Th1-type immune response, may restrict an individual inflammatory response in the liver.…”

Section: Injection Of Ifn-bmentioning

confidence: 76%

The Detection of IRF-1 Promoter Polymorphisms and Their Possible Contribution to T Helper 1 Response in Chronic Hepatitis C

Saito

Tada²,

Wakabayashi³

et al. 2002

Journal of Interferon & Cytokine Research

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We described the interferon (IFN) regulatory factor-1 (IRF-1) promoter single nucleotide polymorphisms (SNPs), and the clinical and immunologic implications of these SNPs have been investigated. We successfully determined the mutation at -300 of the IRF-1 promoter by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and this mutation linked with other mutations in the promoter region. In our Japanese population, the frequency of the type -300*A/A was 11.9%, type A/G was 54.2%, and type G/G was 33.9%. We found no significant difference without IFN stimulation in the production levels of IFN-gamma and interleukin-10 (IL-10) from peripheral blood mononuclear cells (PBMC) between subjects with -300*A/A and those with other types. IFN-alpha stimulation, however, increased the levels of IFN-gamma significantly and decreased the IL-10 production level significantly only in the subject with -300*A/A type. Flow cytometric analysis showed that the Th1-type CD4(+) cell population was significantly increased by IFN-beta administration only in the patient with chronic hepatitis C with -300*A/A type. These results suggest that the IRF-1 promoter SNP types are positively involved in Th1-type response and, consequently, the -300*A/A type may be beneficial for viral elimination in chronic hepatitis C and IFN therapy.

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Section: Injection Of Ifn-bmentioning

confidence: 76%