Different responses of banana classical AGP genes and cell wall AGP components to low-temperature between chilling sensitive and tolerant cultivars

Abstract

Classical arabinogalactan proteins (AGPs) belong to glycosylphosphatidylinositol-anchored proteins, which are proved to be involved in signaling and cell wall metabolism upon stresses. However, rare information is available on the roles of classical AGPs in low temperature (LT) tolerance. Cultivation of banana in tropical and subtropical region is seriously threatened by LT stress. In the present study, 17 classical MaAGPs and nine MbAGPs in banana A and B genome were identi ed and characterized, respectively. Great diversity was present among different classical MaAGP/MbAGP members while ve members (AGP3/6/11/13/14) showed 100% identity between these two gene families. We further investigated different responses of classical AGPs to LT between a chilling sensitive (CS) and tolerant (CT) banana cultivars. In addition, different changes in the temporal and spatial distribution of cell wall AGP components under LTs between these two cultivars were compared using immuno uorescence labeling. Seven classical MbAGPs were upregulated by LT(s) in the CT cultivar. Classical MaAGP4/6 was induced by LT(s) in both cultivars while MaAGP1/2/9/16/17 only in the CT cultivar. Moreover, these genes showed signi cantly higher transcription abundance in the CT cultivar than the CS one under LT(s) except classical MaAGP4. Similar results were observed with the epitopes of JIM13 and LM2 antibodies. The antigens of these antibodies and classical MaAGP1/2/6/9/16/17 might be related to LT tolerance of banana. These results provide additional information about plant classical AGPs and their involvement in LT tolerance, as well as their potential as candidate genes to be targeted when breeding CT banana. Key MessageSeven-teen classical MaAGPs and nine MbAGPs were identi ed and analyzed. MaAGP1/2/6/9/16/17, the antigens of JIM13 and LM2 antiboides are likely to be involved in banana chilling tolerance.

Arabinogalactan proteins – Multifunctional glycoproteins of the plant cell wall

“…AGPs are now recognised as playing roles in regulating both cell wall structural integrity and cell wall signalling and opens multiple avenues for exploring functional mechanisms and potential applications. Understanding AGPs role in the wall has potential to improve crop tolerance to salt stress and resilience to more variable climates such as low and high temperatures ( Kutsuno et al, 2022 , Liu et al, 2022 ). The role of AGPs in regulating development of reproductive organs and pollination could contribute to overcoming barriers of crossing or self-incompatibility ( Zhou et al, 2022 ).…”

Genome‐wide identification of XTH gene family in Musa acuminata and response analyses of MaXTHs and xyloglucan to low temperature

“…Many previous studies revealed that cell wall components and metabolism genes were reported to be involved in the tolerance of plants to LT stress (Yang et al, 2015; Yan et al, 2015; https://webofscience.clarivate.cn/wos/author/record/2244841 et al, 2019; Meng et al, 2020; Yuan et al, 2021; Liu et al, 2022; Kutsuno et al, 2023). However, no studies were focused on the systemic response of MaXTHs to LT stress.…”