Different responses of protein synthesis to refeeding in various muscles of fasted chicks

Abstract

1. The change in the rate of protein synthesis of different muscles, concentrations of plasma insulin, plasma insulin-like growth factor-I (IGF-I) and other plasma components were investigated after refeeding in fasted chicks. 5.2 g of the complete diet was refed. This was the maximum that could be force-fed with water. 2. The fractional synthesis rates (FSR) of breast (M. pectoralis major) and leg (M. gastrocnemius) muscles were measured after injection of L-[2, 6-(3)H]phenylalanine. Plasma insulin and IGF-I concentration were determined by radioimmunoassay. 3. In the breast muscle, FSR was significantly reduced by 2-d fasting. The FSR had recovered completely after 1 h of refeeding and was maintained until 6 h. The change in FSR after refeeding was associated with the change in ribosomal efficiency (K(RNA); absolute synthesis rate per unit RNA), while no change in ribosomal capacity (C(S); RNA: protein ratio) was observed. 4. In the leg muscle, FSR was decreased by 2-d fasting and increased gradually toward 6 h after refeeding but did not reach the level of the fed control. In contrast to the breast muscle, no significant changes in Cs and K(RNA) in the leg muscle were observed. 5. Plasma glucose concentration increased significantly at 1 h after refeeding but returned to the fasted level after 24 h. Plasma insulin concentration in chicks refed for 1 h was higher than in the fasted group. There was no significant change in plasma IGF-I concentration. 6. These results suggest that the FSR of breast muscle was more sensitive to refeeding than that of leg muscle which may be explained, in part, by differences in sensitivity to the change in circulating plasma insulin concentration after refeeding.

Effect of feeding different levels of Azolla pinnata on blood biochemicals, hematology and immunocompetence traits of Chabro chicken

“…Total plasma proteins are a common parameter utilized to estimate the avian body condition. Moreover, albumin, one of the main serum proteins, serves as the most favorable source of amino acids for synthesis of tissue proteins [30]. Similar total protein and albumin content in the blood of all the treatment groups in our study represented normal body condition of all the experimental groups.…”

Effect of in ovo feeding and its interaction with timing of first feed on glycogen reserves, muscle growth, and body weight

“…The posthatch enhancing effects of IOF and EF, compared with the adverse effects of delayed first feeding, on muscle growth could be attributed to increased liver and muscle glycogen reserves , enhanced muscle protein synthesis (Yaman et al, 2000), accelerated development or better functionality of the small intestine (Tako et al, 2004;Smirnov et al, 2006;Foye et al, 2007), a higher reservoir of muscle progenitors (satellite cells; Halevy et al, 2000Halevy et al, , 2003Mozdziak et al, 2002a;Moore et al, 2005a,b), or their combination. Conceivably, adequate glycogen reserves and sufficient protein synthesis are crucial for higher functionality of the GIT, and hence for improved body and muscle growth.…”

Physiological and Behavioral Responses of Young Chicks to High Ambient Temperature

“…Rajman et al (2006) reported that feed deprivation tended to decrease triacylglycerol, total plasma protein and cholesterol concentrations. Yaman et al (2000) described similar triacylglycerol pattern in growing feed-deprived chicks while plasma cholesterol was not influenced. Triacylglycerol concentration was decreased with travel distance at summer temperature (Vosmerova et al, 2010).…”