Different Responses to N+ Beam Implantation Between Diploid and Autotetraploid Rice

Abstract

The objective of the study is to investigate the biological effects of N(+) beam implantation in different ploidy rice. N(+) beam implantation had increase effect in tillers number, spikelet fertility, grain yield per plant, si-phellem cell size, photosynthetic rate, transpiration rate, flag leaf dry weight, flag leaf culm dry weight, stomatal length, vascular bundle area, and protein and starch content and decrease effect in 1,000-grain weight, stomatal width and chlorophyll, calcium, sodium, and zinc content for all rice lines. N(+) beam implantation had opposite effect on diploid and autotetraploid rice lines in vascular bundle area, stomatal complexes areas, epidermal cell size, era length, area of air spaces, midrib length, papilla number, flag leaf length, flag leaf width, flag leaf area, and cadmium, copper, ferrum, magnesium and phosphorus content. Twenty traits of diploid line and ten traits of autotetraploid line are significantly increased by N(+) beam in this experiment, ranging from 6.8 to 362.7 % in diploid line and 7.9 to 131.7 % in autotetraploid line. Six traits of diploid lines and 15 traits of autotetraploid line are significantly decreased by N(+) beam implantation in this experiment, ranging from 8.9 to 87.4 % in diploid line and 5.6 to 88.5 % in autotetraploid line.

The mechanism of starch content increase in grain of autotetraploid rice (Oryza sativa L.)

“…After maturation, the 1000-grain masses of 630-4X and SP-4X was 50.8 and 53.1 g, respectively. Moreover, the 630-2X and SP-2X had 1000-grain masses that were 29.2 and 25.1 g, respectively (Yang et al 2013). The kernel masses of two autotetraploid rice lines were significantly higher compared to those of corresponding diploid rice lines.…”

Physiologic and molecular responses of indica–japonica subspecies tetraploid rice seed germination to ion beams

“…N + beam irradiation can alter many characteristics of rice, but there are apparent differences in various ploidy lines and various individual plants of the same line 21 . An appropriate dose of ionizing radiation can induce changes in the activity of antioxidant enzymes and esterase, reduce the accumulation of malondialdehyde (MDA) and help maintain typical membrane structure and metabolism 22 .…”