Different serum soluble Fas levels in patients with allergic rhinitis and bronchial asthma

Nakashima, Izumi; Kato, Masashi; Nozaki, Yuji; Yoshimoto, T.; Tamada, Yasuhiko; Kageyama, Makoto; Yamashita, Tetsuji; Kurimoto, Fumihiko

doi:10.1034/j.1398-9995.1999.00303.x

Cited by 21 publications

(14 citation statements)

References 15 publications

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“…In this study, we demonstrated significantly lower serum sFas in allergic asthmatic patients as compared to nonatopic subjects. Our observation is line with previous study of Kato et al 27 who described lower levels of serum sFas in patients with symptomatic allergic rhinitis as compared to healthy persons. Jayaraman S, et al 28 observed, that asthmatic subjects had 23% lower levels of Fas + T cells (in the airway mucosa) during treatment with glucocorticoids and suggested that selective resistance to Fas-dependent apoptosis may reflect altered antigen-driven, accessory celldependent signaling.…”

Section: Discussionsupporting

confidence: 95%

Increased apoptosis of peripheral blood mononuclear cells in patients with perennial allergic asthma/rhinitis: relation to serum markers of apoptosis

Grzegorczyk

Kowalski

Piłat

et al. 2002

Mediators of Inflammation

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BACKGROUND: The goal of our study was to examine spontaneous and stimulated apoptosis of peripheral blood MNC from allergic patients, sensitized to Der p I antigen as compared to cells from non-atopic subjects. Furthermore we aimed to investigate which populations of mononuclear cells (lymphocytes, monocytes) undergo the apoptosis and to determine relations between apoptosis and serum levels of sFas/APO-1, ICE/caspase-1 or TNF-alpha. METHODS: The study included 17 patients with perennial, allergic asthma and/or allergic rhinitis [6 male and 11 female; mean age 29,5 years; (range 15-49)]. Apoptosis was assessed by fluorescence technique and confirmed by flow-cytometric method and DNA ladder. Serum levels of sFas, ICE/caspase-1 or TNF-alpha were determined by immunoassays (ELISA). RESULTS: Apoptotic index of unfractionated mononuclear cells (MNC) and lymphocytes (but not monocytes) were significantly higher in allergic patients as compared to non-allergic subjects after 48 and 72 hours of culture (p<0.05). Incubation of cells with ConA (10 microg/ml) resulted in a significant increase in the proportion of apoptotic cells in all populations once the apoptotic index for MNC and lymphocytes (but not monocytes) was again significantly higher in allergic as compared to non-allergic subjects after 24, 48 and 72 hour of culture. In allergic patients, mean serum sFas level, was significantly lower then in non-allergic group (mean value 624.8 pg/ml +/- 25.67 versus 802.0 pg/ml +/- 31.91; p = 0.003) and in both groups sFas level correlated inversely with apoptosis of MNC. The mean ICE/caspase-1 concentration was significantly higher in sera of allergic patients as compared to non-allergic group (mean value 27.71 pg/ml +/- 3.79 vs 23.54 pg/ml respectively; p<0.01). ICE/caspase-1 levels in allergic patients correlated with apoptotic index of mononuclear cells (r = 0.57; p<0.001). CONCLUSIONS: An increased spontaneous and mitogen-induced apoptosis of MNC from peripheral blood of atopic patients as well as different serum levels of sFas and ICE/caspase-1 correlating with apoptosis, suggest different regulation of apoptotic process in peripheral blood mononuclear cells of patients with allergic asthma and/or rhinitis.

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Section: Discussionsupporting

confidence: 95%

Increased apoptosis of peripheral blood mononuclear cells in patients with perennial allergic asthma/rhinitis: relation to serum markers of apoptosis

Grzegorczyk

Kowalski

Piłat

et al. 2002

Mediators of Inflammation

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“…In contrast, serum sFas levels in patients with bronchial asthma are increased during the attack phase. 32 These findings, together with our observations, indicate that serum sFas concentration is an unpredictable marker in allergic or other hypersensitive diseases, but it is a potential indicator of immunologic activation in CD.…”

Section: Discussionsupporting

confidence: 56%

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease

Augustin¹,

Kokkonen

Karttunen³

et al. 2005

Journal of Allergy and Clinical Immunology

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“…Contradictory observations exist concerning the concentration of serum soluble Fas and FasL (sFas and sFasL) in patients with allergic rhinitis and bronchial asthma [5,6].…”

Section: Introductionmentioning

confidence: 99%

Association of TLR-4 and TNF-alpha polymorphisms with clinical symptoms and cytokine levels in patients with allergic rhinitis

Krasznai

Szaniszlo

Kraxner

et al. 2010

Eur Arch Otorhinolaryngol

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Our objective was to determine the frequency of TNF-alpha -238, -308 G/A promoter and TLR-4 299 D/G and 399 T/I polymorphisms in healthy population and in patients with seasonal allergic rhinitis, and to examine its influences on serum TNF-alpha, TNF receptor-1, Fas, Fas-ligand, IgE levels and on clinical symptoms. A pilot study was performed in 66 patients with seasonal allergic rhinitis to ragweed pollen and 161 non-allergic subjects using PCR-RFLP technique and ELISA. Carriers of the -238A and -308G alleles have significantly higher TNF-alpha and IgE levels, clinical score values and lower peak nasal flow (PNIF) values during and after ragweed pollen season. Patients with the 299G/399I alleles of the TLR-4 gene have significantly lower TNF-alpha, Fas, FasL and IgE levels, clinical scores and higher PNIF values during and after pollen season. The -238A and -308G polymorphisms of the TNF-alpha promoter and 299D/399T polymorphisms of the TLR-4 gene are associated with more pronounced clinical symptoms, higher cytokine and IgE levels, and low PNIF values. These polymorphisms are very likely to contribute to the heterogeneity of clinical and laboratory parameters of patients.

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Different serum soluble Fas levels in patients with allergic rhinitis and bronchial asthma

Abstract: Our results suggest a different pathogenesis for allergic rhinitis and bronchial asthma at the cell apoptosis-linked step.

Cited by 21 publications

References 15 publications

Increased apoptosis of peripheral blood mononuclear cells in patients with perennial allergic asthma/rhinitis: relation to serum markers of apoptosis

Increased apoptosis of peripheral blood mononuclear cells in patients with perennial allergic asthma/rhinitis: relation to serum markers of apoptosis

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease

Association of TLR-4 and TNF-alpha polymorphisms with clinical symptoms and cytokine levels in patients with allergic rhinitis

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