1998
DOI: 10.1080/15216549800204522
|View full text |Cite
|
Sign up to set email alerts
|

Different sources of acidity in glucose‐elicited extracellular acidification in the yeast Saccharomyces cerevisiae

Abstract: Three wild‐type strains of Saccharomyces cerevisiae, viz. K, Y55 and Σ1278b, two mutants lacking one or both of the putative K+ transporters, trklΔ and trklΔtrk2Δ, and a mutant in the plasma membrane H+‐ATPase, viz. pmal‐105, were compared in their extracellular acidification following addition of glucose and subsequent addition of KCl; in ATPase activity in purified plasma membranes; and in respiration on glucose. The glucose‐induced acidification was the greater the higher the respiratory quotient, i.e. the … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
7
0

Year Published

1999
1999
2023
2023

Publication Types

Select...
8

Relationship

1
7

Authors

Journals

citations
Cited by 9 publications
(7 citation statements)
references
References 11 publications
0
7
0
Order By: Relevance
“…To determine whether ferritin utilization is dependent on fungal-driven acidification, we substituted the glucose in the medium for casamino acids. This mixture of amino acids can be used as a carbon source by yeasts and avoids the acidification associated with glucose use [69],[70]. Furthermore, we stabilized the buffering capacity of the medium by the addition of HEPES buffer (pH 7.4) with increasing concentrations.…”
Section: Resultsmentioning
confidence: 99%
“…To determine whether ferritin utilization is dependent on fungal-driven acidification, we substituted the glucose in the medium for casamino acids. This mixture of amino acids can be used as a carbon source by yeasts and avoids the acidification associated with glucose use [69],[70]. Furthermore, we stabilized the buffering capacity of the medium by the addition of HEPES buffer (pH 7.4) with increasing concentrations.…”
Section: Resultsmentioning
confidence: 99%
“…This enzymic reaction is essential for cell viability because of its function as a proton pump that regulates cytoplasmic pH and provides the driving force for nutrient uptake (Serrano et al, 1986 ;Serrano, 1989). Lapathitis & Kotyk (1998) reported that the glucoseinduced extracellular acidification depends on both proton extrusion resulting from the action of the plasma membrane H + -ATPase and the cellular excretion of glucose-derived carbon dioxide and organic acids. In that study, the extent of contribution of the latter process was examined by using strain Y55 and its isogenic mutant, pmal-105, with a genetic lack of the enzyme.…”
Section: Inhibitory Effects Of Ampc16 On Glucose-induced Extracellulamentioning
confidence: 99%
“…It was shown before (e.g. [3,18]) that the acidification following addition of glucose has several components, that caused by H + ‐ATPase often playing a minor role. Moreover, back‐titration experiments (not presented here) showed that after galactose addition the consumption of alkaline titrant is about one‐third of that after glucose addition.…”
Section: Resultsmentioning
confidence: 99%