Different subsets of haematopoietic cells and immune cells in bone marrow between young and older donors

Yao, Wenlong; Zhao, Hongyan; Tang, Shu-Qian; Zhang, Y.-Y.; Wang, Yu; Xu, Lei; Zhang, X.-H.; Huang, Xiao‐Jun; Kong, Yuan

doi:10.1111/cei.13531

Cited by 6 publications

(8 citation statements)

References 51 publications

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“…Some studies have shown that the adaptive immune system, especially T cells, is more susceptible to ageing than the innate immune system [3,4]. Our and other previous studies showed decreased frequencies of bone marrow (BM) lymphoid progenitor cells and naïve T cells but increased frequencies of memory T cells, as well as unbalanced effector T cell subset differentiation, such as increased T helper (Th)-1 and T cytotoxic (Tc)-1 cells, in elderly individuals [1,2,5]. Emerging evidence has indicated that decreased BM lymphoid progenitor cells and involution of the thymus may be responsible for the alterations in T cell subsets with ageing [6][7][8].…”

Section: Introductionmentioning

confidence: 59%

“…Numerous studies have shown that the occurrence of aGVHD was related to increased frequencies of donor Th1 and Tc1 cells and reduced frequencies of Th2 and Tc2 cells [37,38,41]. Furthermore, we recently reported that young donors had lower percentages of Th1 and Tc1 cells than old donors [5], which could partly explain why patients receiving transplants from young donors showed less aGVHD than those who received transplants from old donors [42,43]. However, the in-depth reason why the aGVHD incidence was affected by donor age remains to be explored.…”

Section: Discussionmentioning

confidence: 91%

“…After 3 days of coculture, CD3 + T cells were treated with cell stimulation cocktail (500×, eBioscience, CA, USA) as the manufacturer's instructions to induce CD3 + T cells activation and cytokine secretion. Then, we evaluated the IL-4, IFN-γ, IL-17 and Foxp3 levels by flow cytometry using the hereinafter monoclonal antibody panel: CD3-APC-H7, CD8-V500, CD25-PE-Cy7, IL-4-PE, IFN-γ-Percpcy5.5, IL-17-FITC, and Foxp3-APC (BD Biosciences) [5,[28][29][30]. The immunophenotyping of T cell subsets including Th1, Th2, Th17, Tc1, Tc2 and regulatory T cells (Tregs) were in keeping with our previous studies [5,[28][29][30][31].…”

Section: Immunophenotype Analysis Of the Cocultured T Cell Subsetsmentioning

confidence: 99%

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Improved function and balance in T cell modulation by endothelial cells in young people

Tang

Yao

Wang

et al. 2021

Preprint

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Elderly individuals exhibit unbalanced bone marrow (BM) effector T cell subset differentiation, such as increased T helper (Th)-1 and T cytotoxic (Tc)-1 cell frequencies, but the underlying mechanism still unclear. Endothelial cells (ECs) , which are instructive components of the BM microenvironment, exhibit the phenotype of semi-professional antigen-presenting cells and regulate T cell recruitment and activation. Thus, we compared the frequency and function of BM ECs, especially their capacity to regulate effector T cell subsets, between young and old healthy individuals, and explored the underlying mechanism of this immunomodulatory discrepancy. Although the young and old EC percentages were comparable, young ECs showed less reactive oxygen species and better migratory and tube-forming abilities than old ECs. Notably, young ECs regulated T cells to differentiate into fewer Th1 and Tc1 cells than old ECs. Reduced T cell activation molecules and inflammatory cytokines in young BM ECs may be the possible mechanism.

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Section: Introductionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 91%

Section: Immunophenotype Analysis Of the Cocultured T Cell Subsetsmentioning

confidence: 99%

See 1 more Smart Citation

Improved function and balance in T cell modulation by endothelial cells in young people

Tang

Yao

Wang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Some studies have shown that the adaptive immune system, especially T cells, is more susceptible to ageing than the innate immune system [3,4]. Our and other previous studies showed decreased frequencies of bone marrow (BM) lymphoid progenitor cells and naive T cells but increased frequencies of memory T cells, as well as unbalanced effector T cell subset differentiation, such as increased T helper type 1 (Th1) and T cytotoxic 1 (Tc1) cells, in elderly individuals [1,2,5]. Emerging evidence has indicated that decreased BM lymphoid progenitor cells and involution of the thymus may be responsible for the alterations in T cell subsets with ageing [6][7][8].…”

Section: Introductionmentioning

confidence: 61%

“…After 3 days of co-culture, CD3 + T cells were treated with cell stimulation cocktail (×500; eBioscience, San Diego, California, USA), as the manufacturer's instructions, to induce CD3 + T cells activation and cytokine secretion. Then, we evaluated the IL-4, IFN-γ, IL-17 and forkhead box protein 3 (FoxP3) levels by flow cytometry using the following monoclonal antibody panel: CD3-APC-H7, CD8-V500, CD25-PE/Cy7, IL-4-PE, IFN-γ-peridinin chlorophyll cyanin 5.5 (PerCPCy5.5), IL-17-FITC and FoxP3-APC (BD Biosciences) [5,[28][29][30]. The immunophenotyping of T cell subsets, including Th1, Th2, Th17, Tc1, Tc2 and regulatory T cells (Tregs), were in keeping with our previous studies [5,[28][29][30][31].…”

Section: Immunophenotype Analysis Of the Cocultured T Cell Subsetsmentioning

confidence: 99%

Improved function and balance in T cell modulation by endothelial cells in young people

Tang

Yao

Wang

et al. 2021

Clinical and Experimental Immunology

Self Cite

View full text Add to dashboard Cite

Elderly individuals exhibit unbalanced bone marrow (BM) effector T cell subset differentiation, such as increased T helper type 1 (Th1) and T cytotoxic type 1 (Tc1) cell frequencies, but the underlying mechanism is still unclear. Endothelial cells (ECs), which are instructive components of the BM microenvironment, exhibit the phenotype of semi-professional antigen-presenting cells and regulate T cell recruitment and activation. Thus, we compared the frequency and function of BM ECs, especially their capacity to regulate effector T cell subsets, between young and elderly healthy individuals, and explored the underlying mechanism of this immunomodulatory discrepancy. Although the young and elderly EC percentages were comparable, young ECs showed fewer reactive oxygen species and better migratory and tube-forming abilities than elderly ECs. Notably, increased T cell activation molecules and inflammatory cytokines were found in elderly ECs which regulated T cells to differentiate into more proinflammatory T cells, including Th1 and Tc1 cells, than young ECs.

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Bone marrow macrophages are involved in the ineffective hematopoiesis of myelodysplastic syndromes

Xing,

Yao,

Zhao

et al. 2024

Journal Cellular Physiology

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Myelodysplastic syndromes (MDS) are a group of heterogeneous myeloid clonal disorders characterized by ineffective hematopoiesis. Accumulating evidence has shown that macrophages (MΦs) are important components in the regulation of tumor progression and hematopoietic stem cells (HSCs). However, the roles of bone marrow (BM) MΦs in regulating normal and malignant hematopoiesis in different clinical stages of MDS are largely unknown. Age‐paired patients with lower‐risk MDS (N = 15), higher‐risk MDS (N = 15), de novo acute myeloid leukemia (AML) (N = 15), and healthy donors (HDs) (N = 15) were enrolled. Flow cytometry analysis showed increased pro‐inflammatory monocyte subsets and a decreased classically activated (M1) MΦs/alternatively activated (M2) MΦs ratio in the BM of patients with higher‐risk MDS compared to lower‐risk MDS. BM MФs from patients with higher‐risk MDS and AML showed impaired phagocytosis activity but increased migration compared with lower‐risk MDS group. AML BM MΦs showed markedly higher S100A8/A9 levels than lower‐risk MDS BM MΦs. More importantly, coculture experiments suggested that the HSC supporting abilities of BM MΦs from patients with higher‐risk MDS decreased, whereas the malignant cell supporting abilities increased compared with lower‐risk MDS. Gene Ontology enrichment comparing BM MΦs from lower‐risk MDS and higher‐risk MDS for genes was involved in hematopoiesis‐ and immunity‐related pathways. Our results suggest that BM MΦs are involved in ineffective hematopoiesis in patients with MDS, which indicates that repairing aberrant BM MΦs may represent a promising therapeutic approach for patients with MDS.

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Different subsets of haematopoietic cells and immune cells in bone marrow between young and older donors

Abstract: The differences in the frequency and immune differentiation potential of HSCs in BM between young donors and older donors may partly explain the different outcomes of allo‐HSCT.

Cited by 6 publications

References 51 publications

Improved function and balance in T cell modulation by endothelial cells in young people

Improved function and balance in T cell modulation by endothelial cells in young people

Improved function and balance in T cell modulation by endothelial cells in young people

Bone marrow macrophages are involved in the ineffective hematopoiesis of myelodysplastic syndromes

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