Different substrate specificities of two triazine hydrolases (TrzNs) from<i>Nocardioides</i>species

Yamazaki, Kaoru; Fujii, Kunihiko; Iwasaki, Akio; Takagi, Kazuhiro; Satsuma, Koji; Harada, Naoki; Uchimura, Tai

doi:10.1111/j.1574-6968.2008.01271.x

Cited by 28 publications

(24 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nocardioides sp. strain MTD22 was capable of decomposing a wide range of s-triazine herbicides and accumulating cyanuric acid (Yamazaki et al 2008a). Incomplete s-triazine-degrading bacteria, such as strain FJ1117YT, often require co-culturing with other degrading bacteria and/or some accompanying bacteria to complete the degradation and utilization of s-triazine compounds for their growth (Yamazaki et al 2008b).…”

Section: Discussionmentioning

confidence: 99%

“…One gram of the soil was mixed with 3 g of charcoal A100 (provided by Toyo Denka Kogyo, Kochi, Japan) and set in a perfusion apparatus as described by Audus (1946). A 10-μL loop of s-triazine-degrading bacteria previously isolated in our laboratory, including strain CDB21 ), strain FJ1117YT , and strain MTD22 (Yamazaki et al 2008a), were inoculated into the perfusion apparatus. Bacteria accompanying the s-triazine-degrading strains, Bradyrhizobium japonicum strain CSB1 (isolated with strain CDB21, Yamazaki et al 2008b) and Hyphomicrobium sp.…”

Section: Enrichment and Isolation Of The Melamine-degrading Bacteriummentioning

confidence: 99%

“…The PCR products were purified using a QIAquick PCR Purification Kit (QIAGEN). The cycle sequencing reactions were then performed using a Thermo Sequenase Primer Cycle Sequencing Kit (GE Healthcare UK Ltd., Buckinghamshire, UK) with the following 5′-Texas Red-labeled primers: fD1, 341f, 534r, 907fT, 907rA, 1223r, and rP1 (Yamazaki et al 2008a). The PCR products were then analyzed using a SQ5500E DNA Sequencer (Hitachi Ltd., Tokyo, Japan).…”

Section: Identification Of the Isolated Bacteriamentioning

confidence: 99%

“…The PCR detection of genes encoding for s-triazinedegrading enzymes (atzsA, B, C, D, E, F, and trzN) in the melamine-degrading isolate was performed using specific primers, as described previously (Yamazaki et al 2008a) with a slight modification to the primer pair for amplifying atzD. The primers for atzD were atzDf2 (5′-TCCCACCT GACATCACAAAC-3′) and atzDr2 (5′-GGGTCTCGAG GATTTGATTG.-3′).…”

Section: Detection Of Genes Encoding S-triazine-degrading Enzymesmentioning

confidence: 99%

“…strain FJ1117YT; and a wide ranging s-triazine-degrading Nocardioides sp. strain MTD22 Fujii et al 2007;Yamazaki et al 2008a). Strain CDB21 holds genes atzsA, B, C, D, E, and F which encode for the atrazinecatabolizing enzymes, atrazine chlorohydrolase (AtzA); hydroxyatrazine n-ethylaminohydrolase (AtzB); nisopropylammelide aminohydrolase (AtzC); cyanuric acid amidohydrolase (AtzD); biuret hydrolase (AtzE); and allophanate hydrolase (AtzF).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Biodegradation of melamine and its hydroxy derivatives by a bacterial consortium containing a novel Nocardioides species

Takagi

Fujii

Yamazaki

et al. 2011

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Melamine has recently been recognized as a food contaminant with adverse human health effects. Melamine contamination in some crops arises from soil and water pollution from various causes. To remove melamine from the polluted environment, a novel bacterium, Nocardioides sp. strain ATD6, capable of degrading melamine was enriched and isolated from a paddy soil sample. The enrichment culture was performed by the soil-charcoal perfusion method in the presence of triazine-degrading bacteria previously obtained. Strain ATD6 degraded melamine and accumulated cyanuric acid and ammonium, via the intermediates ammeline and ammelide. No gene known to encode for triazine-degrading enzymes was detected in strain ATD6. A mixed culture of strain ATD6 and a simazine-degrading Methyloversatilis sp. strain CDB21 completely degraded melamine, but the degradation rate of cyanuric acid was slow. The degradation of melamine and its catabolites by the mixed culture was greatly enhanced by including Bradyrhizobium japonicum strain CSB1 in the inoculum and adding ethanol to the culture medium. The melamine-degrading consortium consisting of strains ATD6, CDB21, and CSB1 appears to be potentially safer than other known melamine-degrading bacteria for the bioremediation of farmland and other contaminated sites, as no known pathogens were included in the consortium.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Enrichment and Isolation Of The Melamine-degrading Bacteriummentioning

confidence: 99%

Section: Identification Of the Isolated Bacteriamentioning

confidence: 99%

Section: Detection Of Genes Encoding S-triazine-degrading Enzymesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Biodegradation of melamine and its hydroxy derivatives by a bacterial consortium containing a novel Nocardioides species

Takagi

Fujii

Yamazaki

et al. 2011

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

Nocardioides

Evtushenko¹,

Krausova²,

Yoon³

2015

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

No.car.di.o.i'des. N.L. fem. n. Nocardia name of a genus; L. suff. ‐ oides (from Gr. suff. ‐ eides from Gr. n. eidos , that which is seen, form, shape, figure) ressembling, similar; N.L. masc. n. Nocardioides Nocardia ‐like, referring to the similarity of life cycles of the type species of this genus and Nocardia . Actinobacteria / Actinobacteria / Propionibacteriales / Nocardioidaceae / Nocardioides Abundantly branched vegetative hyphae or irregular rods may be formed in young cultures. The morphogenetic cycle is usually observable, with different organisms showing more or less complex succession of morphological stages . The morphogenetic cycle usually starts with the coccoid cells or short rods which may simply germinate into rods or longer filaments, show elementary branching or form extensively branched hyphae on and below the surface of agar media, and may give rise to aerial mycelium . The latter consists of irregular, sparsely branching or unbranched hyphae and may totally or partially cover the primary mycelium, or be discernible only microscopically. Both the vegetative and aerial hyphae and the rod‐shaped cells undergo various degrees of fragmentation (division via septa formation). Fragmentation finally results in the next generation of short rod‐like and coccoid cells. No endospores are formed. Rod‐shaped bacteria may be motile. Gram‐stain‐positive type of cell wall. Non‐acid‐fast. The colony color is mainly whitish, creamy, or yellow of different tint and intensity and rarely orange . Diffusible pigments are not usually produced. Colonies not covered by aerial mycelium are mostly pasty , with smooth to wrinkled surface . Chemo‐organotrophic, with an oxidative type of metabolism. Predominantly catalase‐positive. The level of oxidase activity varies among species. Grows under aerobic conditions on standard laboratory media, including chemically defined (synthetic) media or media with low nutrient concentrations. Certain vitamins or other growth factors may be required. Utilizes a wide range of carbon and nitrogen sources, including unusual organic compounds and toxic environmental pollutants. Mostly mesophilic and neutrophilic; some grow at initial pH values of 5–5.5 and/or 11–12. Mostly non‐halophilic, but salt‐requiring organisms occasionally occur. DNA G + C content ( mol %): 67.5 ( T m )–74.8 (HPLC). Type species : Nocardioides albus Prauser 1976, 61 AL .

show abstract

Agronomic and environmental implications of enhanceds-triazine degradation

et al. 2010

View full text Add to dashboard Cite

environmental implications of enhanced s-triazine degradation" (2010 AbstractNovel catabolic pathways enabling rapid detoxification of s-triazine herbicides have been elucidated and detected at a growing number of locations. The genes responsible for s-triazine mineralization, i.e. atzABCDEF and trzNDF, occur in at least four bacterial phyla and are implicated in the development of enhanced degradation in agricultural soils from all continents except Antarctica. Enhanced degradation occurs in at least nine crops and six crop rotation systems that rely on s-triazine herbicides for weed control, and, with the exception of acidic soil conditions and s-triazine application frequency, adaptation of the microbial population is independent of soil physiochemical properties and cultural management practices. From an agronomic perspective, residual weed control could be reduced tenfold in s-triazine-adapted relative to non-adapted soils. From an environmental standpoint, the off-site loss of total s-triazine residues could be overestimated 13-fold in adapted soils if altered persistence estimates and metabolic pathways are not reflected in fate and transport models. Empirical models requiring soil pH and s-triazine use history as input parameters predict atrazine persistence more accurately than historical estimates, thereby allowing practitioners to adjust weed control strategies and model input values when warranted. Published

show abstract

Different substrate specificities of two triazine hydrolases (TrzNs) fromNocardioidesspecies

Cited by 28 publications

References 23 publications

Biodegradation of melamine and its hydroxy derivatives by a bacterial consortium containing a novel Nocardioides species

Biodegradation of melamine and its hydroxy derivatives by a bacterial consortium containing a novel Nocardioides species

Nocardioides

Agronomic and environmental implications of enhanceds-triazine degradation

Contact Info

Product

Resources

About