Different synovial vasculogenic profiles of primary, rapidly destructive and osteonecrosis-induced hip osteoarthritis. An immunohistochemistry study

Gurzu, Simona; Turdean, Sabin Gligore; Pop, Tudor Sorin; Zazgyva, Ancuţa; Roman, Ciprian Oliviu; Opriş, Mihaela; Jung, Ioan

doi:10.1007/s00264-016-3302-4

Cited by 10 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have shown that VEGF can promote vascular proliferation, enhance vascular permeability and that VEGF levels are closely related to the progression of RA (Gurzu et al, 2017). In the present study, low levels of VEGF expression was found in the synovium of Control rats, suggesting that this factor plays a role in maintaining the normal function of the synovium.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Alterations in the Gut Microbiota of Collagen-Induced Arthritis Rats Following the Prolonged Administration of Total Glucosides of Paeony

Peng

Xie

et al. 2019

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Rheumatoid arthritis (RA) is a common autoimmune disease linked to chronic inflammation. Dysbiosis of the gut microbiota has been proposed to contribute to the risk of RA, and a large number of researchers have investigated the gut-joint axis hypothesis using the collagen-induced arthritis (CIA) rats. However, previous studies mainly involved short-term experiments; very few used the CIA model to investigate changes in gut microbiota over time. Moreover, previous research failed to use the CIA model to carry out detailed investigations of the effects of drug treatments upon inflammation in the joints, hyperplasia of the synovium, imbalance in the ratios of Th1/Th2 and Th17/Treg cells, intestinal cytokines and the gut microbiota following long-term intervention. In the present study, we carried out a 16-week experiment to investigate changes in the gut microbiota of CIA rats, and evaluated the modulatory effect of total glucosides of paeony (TGP), an immunomodulatory agent widely used in the treatment of RA, after 12 weeks of administration. We found that taxonomic differences developed in the microbial structure between the CIA group and the Control group. Furthermore, the administration of TGP was able to correct 78% of these taxonomic differences, while also increase the relative abundance of certain forms of beneficial symbiotic bacteria. By the end of the experiment, TGP had reduced body weight, thymus index and inflammatory cell infiltration in the ankle joint of CIA rats. Furthermore, the administration of TGP had down-regulated the synovial content of VEGF and the levels of Th1 cells and Th17 cells in CIA rats, and up-regulated the levels of Th2 cells and Treg cells. The administration of TGP also inhibited the levels of intestinal cytokines, secretory immunoglobulin A (SIgA) and Interferon-γ (IFN-γ). In conclusion, the influence of TGP on dynamic changes in gut microbiota, along with the observed improvement of indicators related to CIA symptoms during 12 weeks of administration, supported the hypothesis that the microbiome may play a role in TGP-mediated therapeutic effects in CIA rats. The present study also indicated that the mechanism underlying these effects may be related to the regulation of intestinal mucosal immunity remains unknown and deserves further research attention.

show abstract

Section: Discussionmentioning

confidence: 99%

Dynamic Alterations in the Gut Microbiota of Collagen-Induced Arthritis Rats Following the Prolonged Administration of Total Glucosides of Paeony

Peng

Xie

et al. 2019

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The approbative effects of bioactive glasses implants are not limited to osseous regeneration, as it was shown that they induce an increased vascularity in bone lesions of earlier irradiated calvaria [16]. Bioactive Glass seems to be a very good solution for treating osteomyelitis, traumatic pseudarthrosis, revision prosthesis interventions or other pathologies involving bone tissue resorbtion or bone defects [18, [25][26][27][28][29]. This scaffold seems to be a proper substitute for other treatment in hip revision arthroplasty [23,24].…”

Section: Resultsmentioning

confidence: 99%

Results after Using Bioactive Glass S53P4 in Reconstructive Orthopaedic Surgery - A 36 - Month Follow-up Clinical Study

Bățagă¹,

Trâmbiţaş²,

Solyom³

et al. 2019

Rev. Chim.

View full text Add to dashboard Cite

Osteocartilaginous tissue normally has the capability of regeneration, which means that many osteoarticular problems or bony defects will heal spontaneously, not needing important interventions. Although the high regenerative capacity of this tissue, large osseous defects encountered difficulties in optimal healing process and remain a clinical challenge, demanding surgical procedures. This aim of this study was to evaluate clinical and radiological outcomes using bioactive glass S53P4 (BonAlive�) as a synthetic bone substitute material in some specific orthopedic pathologies like prosthesis revision surgery, and pseudarthrosis.

show abstract

“…from the vascularised synovium and that there was an invasion of synovium into the articular cartilage [ 43 ]. Furthermore, in a study investigating the role of angiogenesis in hip OA, it was noted that the grade of angiogenesis was related to the cartilage degeneration, hence it could be involved in the degenerative process [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Differential protein expression in human knee articular cartilage and medial meniscus using two different proteomic methods: a pilot analysis

Folkesson

Turkiewicz

Englund

et al. 2018

BMC Musculoskelet Disord

View full text Add to dashboard Cite

BackgroundProteomics is an emerging field in the study of joint disease. Our two aims with this pilot analysis were to compare healthy human knee articular cartilage with meniscus, two tissues both known to become affected in the osteoarthritic disease process, and to compare two mass spectrometry (MS)-based methods: data-dependent acquisition (DDA) and data-independent acquisition (DIA).MethodsHealthy knee articular cartilage taken from the medial tibial condyle and medial meniscus samples taken from the body region were obtained from three adult forensic medicine cases. Proteins were extracted from tissue pieces and prepared for MS analysis. Each sample was subjected to liquid chromatography (LC)-MS/MS analysis using an Orbitrap mass spectrometer, and run in both DDA and DIA mode. Linear mixed effects models were used for statistical analysis.ResultsA total of 653 proteins were identified in the DDA analysis, of which the majority was present in both tissue types. Only proteins with quantitation information in both tissues (n = 90) were selected for more detailed analysis, of which the majority did not statistically significantly differ in abundance between the two tissue types, in either of the MS analyses. However, 21 proteins were statistically significantly different (p < 0.05) between meniscus and cartilage in the DIA analysis. Out of these, 11 proteins were also significantly different in the DDA analysis. Aggrecan core protein was the most abundant protein in articular cartilage and significantly differed between the two tissues in both methods. The corresponding protein in meniscus was serum albumin. Dermatopontin exhibited the highest meniscus vs articular cartilage ratio among the statistically significant proteins. The DIA method led to narrower confidence intervals for the abundance differences between the two tissue types than DDA.ConclusionsAlthough articular cartilage and meniscus had similar proteomic composition, we detected several differences by MS. Between the two analyses, DIA yielded more precise estimates and more statistically significant different proteins than DDA, and had no missing values, which makes it preferable for future LC-MS/MS analyses.Electronic supplementary materialThe online version of this article (10.1186/s12891-018-2346-6) contains supplementary material, which is available to authorized users.

show abstract

Different synovial vasculogenic profiles of primary, rapidly destructive and osteonecrosis-induced hip osteoarthritis. An immunohistochemistry study

Cited by 10 publications

References 12 publications

Dynamic Alterations in the Gut Microbiota of Collagen-Induced Arthritis Rats Following the Prolonged Administration of Total Glucosides of Paeony

Dynamic Alterations in the Gut Microbiota of Collagen-Induced Arthritis Rats Following the Prolonged Administration of Total Glucosides of Paeony

Results after Using Bioactive Glass S53P4 in Reconstructive Orthopaedic Surgery - A 36 - Month Follow-up Clinical Study

Differential protein expression in human knee articular cartilage and medial meniscus using two different proteomic methods: a pilot analysis

Contact Info

Product

Resources

About