Different vimentin expression in two clones derived from a human colocarcinoma cell line (LoVo) showing different sensitivity to doxorubicin

Conforti, G; Codegoni, Anna Maria; Scanziani, Eugenio; Dolfini, E.; Dasdia, T; Calza, M.; Caniatti, M.; Broggini, Massimo

doi:10.1038/bjc.1995.101

Cited by 18 publications

(14 citation statements)

References 28 publications

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“…In a 5-day MTT cytotoxicity assay, LoVo/C7 cells displayed low-level cross-resistance to VP-16 and VCR compared with LoVo/C5 cells, whereas no differences were observed between the two clonal lines regarding L-PAM and camptothecin. This pattern suggests a typical form of MDR; however, no P-gp overexpression was detected in LoVo/C7 cells, both by Northern blot analysis (Conforti et al, 1995) and according to the immunocytochemical and confocal microscopic data presented in this paper. As expected, LoVo/DX cells expressed significant levels of P-gp and were found to be highly cross-resistant to VP-16 and VCR.…”

Section: Discussionsupporting

confidence: 61%

Characterization of a clonal human colon adenocarcinoma line intrinsically resistant to doxorubicin

Dolfini

Dasdia²,

Arancia³

et al. 1997

Br J Cancer

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Summary Intrinsic low-level resistance to anti-cancer drugs is a major problem in the treatment of gastrointestinal malignancies. To address the problem presented by intrinsically resistant tumours, we have isolated two monoclonal lines from LoVo human colon adenocarcinoma cells: LoVo/C7, which is intrinsically resistant to doxorubicin (DOX); and LoVo/C5, which shows the same resistance index for DOX as the mixed parental cell population. For comparison, we have included in the study a LoVo-resistant line selected by continuous exposure to DOX and expressing a typical multidrug resistant (MDR) phenotype. In these cell lines we have studied the expression and/or activity of a number of proteins, including P-glycoprotein 170 (P-gp), multidrug resistance-associated protein (MRP), lung resistance-related protein (LRP), glutathione (GSH)-dependent enzymes and protein kinase C (PKC) isoforms, which have been implicated in anti-cancer drug resistance. Intracellular DOX distribution has been assessed by confocal microscopy. The results of the present study indicate that resistance in LoVo/C7 cells cannot be attributed to alterations in P-gp, LRP or GSH/GSH-dependent enzyme levels. Increased expression of MRP, accompanied by alterations in the subcellular distribution of DOX, has been observed in LoVo/C7 cells; changes in PKC isoform pattern have been detected in both intrinsically and pharmacologically resistant cells.

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Section: Discussionsupporting

confidence: 61%

Characterization of a clonal human colon adenocarcinoma line intrinsically resistant to doxorubicin

Dolfini

Dasdia²,

Arancia³

et al. 1997

Br J Cancer

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“…Furthermore, proteomic analysis identified the vimentin gene as one differentially expressed in colorectal cancer compared with the surrounding normal tissue (Alfonso et al, 2005). In regard to chemosensitivity, vimentin expression was higher in colon carcinoma cell clones resistant to doxorubicin, although vimentin alone did not confer resistance (Conforti et al, 1995). Similar findings were reported for a multidrug resistant breast cancer subline (Bichat et al, 1997).…”

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confidence: 73%

Quantitative evaluation of vimentin expression in tumour stroma of colorectal cancer

et al. 2007

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Recent studies have identified vimentin, a type III intermediate filament, among genes differentially expressed in tumours with more invasive features, suggesting an association between vimentin and tumour progression. The aim of this study, was to investigate whether vimentin expression in colon cancer tissue is of clinical relevance. We performed immunostaining in 142 colorectal cancer (CRC) samples and quantified the amount of vimentin expression using computer-assisted image analysis. Vimentin expression in the tumour stroma of CRC was associated with shorter survival. Overall survival in the high vimentin expression group was 71.2% compared with 90.4% in the low-expression group (P ¼ 0.002), whereas disease-free survival for the high-expression group was 62.7% compared with 86.7% for the low-expression group (P ¼ 0.001). Furthermore, the prognostic power of vimentin for disease recurrence was maintained in both stage II and III CRC. Multivariate analysis suggested that vimentin was a better prognostic indicator for disease recurrence (risk ratio ¼ 3.5) than the widely used lymph node status (risk ratio ¼ 2.2). Vimentin expression in the tumour stroma may reflect a higher malignant potential of the tumour and may be a useful predictive marker for disease recurrence in CRC patients.

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“…After fixing with paraformaldehyde, and permeabilisation with Triton X-100, 35 cells were stained with p73 monoclonal antibodies (Oncogene Research) and with fluoresceine-tagged antimouse antibodies (Novocastra). Coverslips were mounted in Mowiol 4-88 (Hoechst, Frankfurt) and observed in an Olympus BX60 fluorescence microscope.…”

Section: Immunofluorescencementioning

confidence: 99%

Effects of inducible overexpression of DNp73α on cancer cell growth and response to treatment in vitro and in vivo

et al. 2005

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The p73 gene has a complex regulation, which leads to the expression of different isoforms, often with opposite biological effects. We have generated in the human colocarcinoma cell line HCT116, expressing a wild-type p53, an inducible DNp73a expressing system. Two clones (HCT116/DN3 and HCT116/DN14), upon doxycycline addition, show a strong expression of DNp73a. In vitro the two DNp73a overexpressing clones grow at similar rate of the control transfected clone (HCT116/8a) and similarly respond to DNA damage. When injected in mice, HCT116/DN3, HCT116/DN14, and HCT116/8a cells grew similarly in the absence or presence of tetracycline. In HCT116/DN3 and HCT116/DN14 tumors, tetracycline induced a strong expression of DNp73a both as mRNA and protein. These results indicate that in this system the overexpression of the DNp73a does not induce a more aggressive phenotype and does not seem to be associated with a reduced response of the cells to treatment with anticancer agents.

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Different vimentin expression in two clones derived from a human colocarcinoma cell line (LoVo) showing different sensitivity to doxorubicin

Cited by 18 publications

References 28 publications

Characterization of a clonal human colon adenocarcinoma line intrinsically resistant to doxorubicin

Characterization of a clonal human colon adenocarcinoma line intrinsically resistant to doxorubicin

Quantitative evaluation of vimentin expression in tumour stroma of colorectal cancer

Effects of inducible overexpression of DNp73α on cancer cell growth and response to treatment in vitro and in vivo

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