Differential abilities of Korean soybean varieties to biosynthesize glyceollins by biotic and abiotic elicitors

Abstract

Glyceollins synthesized in soybeans that are exposed to biotic or abiotic stress have been reported to have health benefits. Considering that glyceollins are synthesized from daidzein via several enzymatic steps and that isoflavone concentration widely varies among soybean varieties, the abilities of 60 soybean cultivars to synthesize glyceollins were compared under different elicitation conditions. Soybeans accumulated glyceollins differentially depending upon the cultivar when elicited with. Contrary to our hypothesis that high isoflavone varieties may accumulate glyceollins more efficiently upon elicitation, glyceollin accumulation in response to fungal elicitation was not related with the concentration of either total isoflavones or daidzein in soybeans. Rather the glyceollin levels were significantly affected by soybean cultivar and most effectively increased by fungal infection. The data suggest that the selection of a strong fungal elicitor and a soybean cultivar with genotype that highly expresses the genes involved in glyceollin biosynthesis is essential for efficient glyceollin production.

Effect of elicitors on the metabolites in the suspension cell culture of Salvia miltiorrhiza Bunge

“…This compound can open the metabolic process of enzymatic activity, which can increase the content of secondary metabolites and sometimes even induce new compounds. Elicitors can be classified as biological and abiotic elicitors, depending on their sources (Park et al 2017). Dong et al (2010) revealed that 6.25-22.25 mg/L salicylic acid (SA) could elicit the accumulation of phenolic compounds.…”

Glyceollins from soybean: Their pharmacological effects and biosynthetic pathways

“…Among eight different fungal strains evaluated for their ability to activate glyceollin synthesis, it was observed that the NRRL 3653 strain of Trichoderma reesei was able to cause glyceollin accumulation in soybean cotyledons to reach 3.763 mg/g [ 163 ]. Evaluation of the synthesis ability of 60 different soybean varieties under various conditions revealed that treatment with Aspergillus sojae resulted in the highest yield of glyceollin, exceeding 8 mg/g [ 164 ]. Under acidic conditions (pH 3.0), glyceollin synthesis was effectively promoted [ 165 ].…”

Characterization of Glyceollins as Novel Aryl Hydrocarbon Receptor Ligands and Their Role in Cell Migration

“…Glyceollins, including glyceollin I, II and III (GI, GII and GIII), are the most important phytoalexins present in soybeans exposed to certain fungi and some abiotic elicitors [5,6]. They serve as a protective system in plants through their antibacterial, antifungal and antinematode actions [7].…”