Differential abundance of IGF1, bile acids, and the genes involved in their signaling in the dominant follicle microenvironment of lactating cows and nulliparous heifers

Sánchez, Ricardo Rubio; Schuermann, Yasmin; Gagnon-Duval, Laurianne; Baldassarre, Hernán; Murphy, Bruce D.; Gévry, Nicolas; Agellon, Luis B.; Bordignon, Vilceu; Duggavathi, Raj

doi:10.1016/j.theriogenology.2014.01.005

Cited by 21 publications

(28 citation statements)

References 52 publications

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“…Designing and validation of primers (Table 1) and qPCR were performed as described previously (Sanchez et al 2014). Samples were run in duplicates and transcript levels for each gene of interest were expressed relative to the average abundance of the reference genes, CYCLOPHILIN and GAPDH.…”

Section: Rna Extraction Rt and Real-time Pcrmentioning

confidence: 99%

Lack of FSH support enhances LIF–STAT3 signaling in granulosa cells of atretic follicles in cattle

et al. 2015

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Subordinate follicles (SFs) of bovine follicular waves undergo atresia due to declining FSH concentrations; however, the signalling mechanisms have not been fully deciphered. We used an FSH-induced co-dominance model to determine the effect of FSH on signalling pathways in granulosa cells of the second-largest follicles (SF in control cows and co-dominant follicle (co-DF2) in FSH-treated cows). The SF was smaller than DF in control cows while diameters of co-DF1 and co-DF2 in FSH-treated cows were similar. The presence of cleaved CASP3 protein confirmed that granulosa cells of SFs, but not of DFs and co-DFs, were apoptotic. To determine the effect of FSH on molecular characteristics of the second-largest follicles, we generated relative variables for the second largest follicle in each cow. For this, variables of SF or co-DF2 were divided by the variables of the largest follicle DF or co-DF1 in each cow. There was higher transcript abundance of MAPK1/3 and AKT1/2/3 but lower abundance of phosphorylated MAPK3/1 in SF than co-DF2 granulosa cells. Abundance of mRNA and phosphorylated protein of STAT3 was higher in granulosa cells of control SF than FSH-treated co-DF2. SF granulosa cells had higher levels of LIFR and IL6ST transcripts, the two receptors involved in STAT3 activation. Further, lower transcript abundance of interleukin 6 receptor (IL6R), another receptor involved in STAT3 activation, indicated that STAT3 activation in SF granulosa cells could be mainly due to leukemia inhibitory factor (LIF) signalling. These results indicate that atresia due to lack of FSH is associated with activated LIF-STAT3 signalling in SF granulosa cells, as FSH treatment reversed such activation. Reproduction (2015) 150 395-403

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Section: Rna Extraction Rt and Real-time Pcrmentioning

confidence: 99%

Lack of FSH support enhances LIF–STAT3 signaling in granulosa cells of atretic follicles in cattle

et al. 2015

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“…Furthermore, oocytes, follicular fluid and granulosa cells can be collected from individual follicles under epidural anesthesia using an adapted ovum pick up system, without the need to euthanize animals (Beg et al, 2001;Arashiro et al, 2013;Sanchez et al, 2014). Based on our experience, IFI is an excellent method to manipulate ligand/receptor signaling in a follicle of known developmental status without interfering with such signals in other follicles or tissues.…”

Section: Intrafollicular Injection (Ifi)mentioning

confidence: 99%

“…Moreover, intrafollicular injection of ligands into the largest or the second largest follicle followed by transrectal ultrasonography or ovariectomy at specific time-points after treatment has been performed to investigate the role of local factors on follicular development and atresia (Ferreira et al, 2011a;Gasperin et al, 2012;Barreta et al, 2015). As an alternative to ovariectomy, ultrasound-guided follicular aspiration may be used to recover follicular fluid and granulosa cells from individual follicles (Sanchez et al, 2014). This technique has the advantage of allowing the reutilization of the cows in additional studies or their return to the farm breeding/production program.…”

Section: Examples Of the Utility Of The Cow In The Study Of Ovarian Fmentioning

confidence: 99%

Methods to study ovarian function in monovulatory species using the cow as a model

Rovani¹,

Gasperin

Ferreira³

et al. 2017

Anim Reprod

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In this review, we discuss the utility of the cow as an in vivo model to study the regulation of ovarian functions in monovular species. It is increasingly becoming clear that besides endocrine control, locally produced factors play pivotal roles during dominant follicle selection, oocyte maturation, ovulation and luteolysis. Although in vitro culture systems have been used to study these processes, definitive understanding the interactions between endocrine and local factors requires appropriate in vivo models. Most of the experimental approaches to study ovarian functions in vivo in large animals are based on the use of ultrasonography and considerable progress in this field has been made during the last thirty years. It has been shown that cows are an excellent model to collect samples (e.g., follicular fluid, granulosa cells, oocytes) from live animals at specific stages of follicular development in order to study mechanisms of intrafollicular factors in a physiological endocrine environment. In addition to support fundamental studies, the cow model has contributed immensely to the refinement of assisted reproductive technologies, which are now widely used not only in farm animals but also in humans.

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“…; Sanchez et al. ). The insulin‐like growth factor binding proteins (IGFBPs) bind IGFs with high affinity and consequently regulate their access to IGF receptors.…”

Section: Introductionmentioning

confidence: 97%

“…; Spicer ; Sanchez et al. ). Within follicles, PAPP‐A is able to degrade IGFBP4 and 5, leaving IGF1 available, and is thus likely an important regulator of follicular growth and selection in cattle (Aad et al.…”

Section: Introductionmentioning

confidence: 99%

Involvement of PAPP‐A and IGFR1 in Cystic Ovarian Disease in Cattle

Rodríguez

Colombero

Amweg

et al. 2015

Reprod Domestic Animals

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Cystic ovarian disease (COD) is one of the main causes of infertility in dairy cattle. It has been shown that intra-ovarian factors, such as members of the insulin-like growth factor (IGF) system, may contribute to follicular persistence. The bioavailability of IGF to initiate its response by binding to specific receptors (IGFRs) depends on interactions with related compounds, such as pregnancy-associated plasma protein A (PAPP-A). The aim of this study was to determine IGFR1 and PAPP-A expression both in follicles at different stages of development and in cysts, to evaluate the roles in the etiopathogenesis of COD in cattle. The mRNA expression of PAPP-A was higher in granulosa cells of large tertiary follicles than in cysts, whereas the protein PAPP-A present in the follicular fluid from these follicles showed no differences. Although no PAPP-A mRNA expression was detected in smaller tertiary follicles, in their follicular fluid, this protease was detected in lesser concentration than in cysts. The mRNA expression of IGFR1 was lower in granulosa cells from cystic follicles than in those from tertiary ones. However, the protein expression of this receptor presented the highest levels in cystic structures, probably to increase the possibility of IGF response. The data obtained would indicate that animals with COD have an altered regulation of the IGF system in the ovary, which could be involved in the pathogenesis of this disease in cattle.

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Differential abundance of IGF1, bile acids, and the genes involved in their signaling in the dominant follicle microenvironment of lactating cows and nulliparous heifers

Cited by 21 publications

References 52 publications

Lack of FSH support enhances LIF–STAT3 signaling in granulosa cells of atretic follicles in cattle

Lack of FSH support enhances LIF–STAT3 signaling in granulosa cells of atretic follicles in cattle

Methods to study ovarian function in monovulatory species using the cow as a model

Involvement of PAPP‐A and IGFR1 in Cystic Ovarian Disease in Cattle

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