Differential accumulation of two size classes of poly(A) associated with messenger RNA during oogenesis in Xenopus laevis

Cabada, Marcelo O.; Darnbrough, Chris; Ford, P. J.; Turner, Philip C.

doi:10.1016/0012-1606(77)90227-5

Cited by 77 publications

(26 citation statements)

References 23 publications

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“…This experiment was controlled internally. The total RNA content increased in early oogenesis, remained approximately constant after fertilisation until the hatching tadpole, and then increased to 9 jig RNA per feeding tadpole ( Figure 4B and unpublished results), in accordance with published values (Cabada et al, 1977;Van Dongen et al, 1981;Smithl et al, 1984;Brown and Littna, 1964 (Brown and Littna, 1966;Ford, 1971) Northern experiment (Figure 3). From densitometric quantitation the signal from an unfertilised egg was equivalent to 5 x 106 preleukemic erythroblastic cells and that from a gastrula embryo of 30-60 000 cells to 5 x 105.…”

Section: Resultssupporting

confidence: 89%

“…The high degree of homology with the c-myc gene of chicken, mouse and human of both the nucleotide and the predicted amino acid sequences again indicates the conservation of this proto-oncogene during evolution and suggests an important common function. Xenopus myc expression during oogenesis and embyronic development Our results show that the Xenopus myc RNA belongs to the class of stable maternal RNAs which are accumulated from very early oogenesis (Rosbash and Ford, 1974;Cabada et al, 1977;Dolecki and Smith, 1979;Golden et al, 1980 (Newport and Kirschner, 1982); soon after the embryo enters the gastrula stage and new poly(A)+ RNAs begin to accumulate. Fertilisation is the start of a progressive degradation of the stably accumulated myc RNA stored in the egg (5 x 106 copies) until gastrula (about 30 000 cells) when a minimum level is reached (10 copies/cell).…”

Section: Resultsmentioning

confidence: 61%

See 1 more Smart Citation

Xenopus myc proto-oncogene during development: expression as a stable maternal mRNA uncoupled from cell division.

Taylor¹,

Gusse²,

Evan³

et al. 1986

The EMBO Journal

104

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A Xenopus cDNA clone highly homologous to the protooncogene c-myc has been isolated and used to derive a homologous probe to study myc expression during embryonic development. Myc RNA is identified as a member of the class of maternal mRNAs expressed before fertilisation. It is highly accumulated from early oogenesis and an unfertilised egg contains 8 pg, about 105-fold the myc content of proliferative somatic cells. After fertilisation a post-transcriptional regulation of the gene is induced and the accumulated myc RNA is degraded (t1/2 = 4 h 20 min) to reach a level at gastrula of 10 transcripts per cell; a value maintained during subsequent embyronic development. The Xenopus myc protein has also been identified by both myc-specific antibodies and hybrid selection experiments. Translation in vitro of Xenopus myc RNA shows that it encodes a 62-kd protein which is also recognised by myc antibodies in oocyte extracts. This protein is accumulated in late oogenesis. The results indicate an unusual uncoupling of myc expression and cell proliferation linked to a stabilisation of the RNA product.

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 61%

Xenopus myc proto-oncogene during development: expression as a stable maternal mRNA uncoupled from cell division.

Taylor¹,

Gusse²,

Evan³

et al. 1986

The EMBO Journal

104

View full text Add to dashboard Cite

show abstract

“…3. Poly(A) sequences of untreated oocytes (both zero time and 11 h-incubation) were composed of two distinct size classes ( distribution is similar to that reported previously for full-grown oocytes (4,24). After 3 h of treatment (Fig.…”

supporting

confidence: 82%

“…Recently, these poly(A)+RNAs have been shown to contain two distinct size classes of poly(A), designated poly(A)L (mean length: 60 nucleotides) and poly(A)s (mean length: 20 nucleotides), each of which accumulates at a different time during oogenesis (4).…”

mentioning

confidence: 99%

Metabolism of Poly (A) Sequences of Maternal mRNA during Progesterone-Induced Maturation of Xenopus laevis Oocytes

Sagata

Shiokawa

Yamana

1980

Cell Struct. Funct.

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ABSTRACT. Changes in the content and the size of poly(A) sequences during progesterone-induced maturation of Xenopus laevis oocytes were determined by [3H]poly(U) hybridization and by labeling with exogenously supplied radioactive adenosine. The poly(A) content increased significantly (by about 20 %) by the time of germinal vesicle breakdown (GVBD), then decreased continuously to one half of the initial content by the end of maturation. Gel electrophoresis revealed that poly(A) sequences were being elongated by 20-30 nucleotides before GVBD except for the smallest class of poly-(A) (ca. 20 nucleotides long), but after GVBD they were extensively degraded which gave rise to the egg poly(A) sequences of quite different sizes. All these changes in poly(A) content and size also occurred in oocytes which either were treated with actinomycin D or enucleated before exposure to progesterone; this signifies that they were not dependent on the nucleus. Labeling experiments on maturing oocytes showed that poly(A) sequences were synthesized actively independent of the concomitant synthesis of RNA even when a substantial amount of the poly(A) sequences was being degraded (after GVBD). The size distribution of these labeled poly(A) sequences was the same as that of steady-state poly(A) measured by [3H]poly(U) binding, and they were associated with non-radioactive heterogeneous high molecular weight RNA. Thus, the present findings indicate that the poly(A) sequences associated with cytoplasmic maternal mRNA were being turned over extensively and that a portion of them was completely degraded during oocyte maturation. This poly(A) turnover also was indicated in . full-grown oocytes which were not treated with progesterone and which showed no changes in the content and size of poly(A) throughout the experimental period.

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“…The sequence of the 110-bp region (Fig. 6) (4). Our data are consistent with the hypothesis proposed by Zaret and Sherman (31,32) The 110-bp transcription terminator region upstream of URA3 acts efficiently in either orientation.…”

Section: Materuils and Methodssupporting

confidence: 90%

Transcription terminator-like element within a Saccharomyces cerevisiae promoter region.

Yarger¹,

Armilei²,

Gorman³

1986

Mol. Cell. Biol.

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We analyzed a cloned fragment of the yeast URA3 promoter region that contains a sequence of DNA capable of fimctioning as a highly efficient transcription terminator. BAL 31 deletions have shown the signal for the transcription termination activity is <110 base pairs and resides between bases 45 and 155 upstream of the URA3 primary ATG codon at base 227. In our in vivo assay system, the DNA fragment is able to terminate transcripts very efficiently in either orientation. The teriniiated transcripts bind to oligodeoxythymidylate cellulose columns and promote the synthesis of full-length cDNAsi suggesting that the transciipts are polyadenylated. The 110-base-pair region contains no sequence resembling termoinator consensus sequences described

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Differential accumulation of two size classes of poly(A) associated with messenger RNA during oogenesis in Xenopus laevis

Cited by 77 publications

References 23 publications

Xenopus myc proto-oncogene during development: expression as a stable maternal mRNA uncoupled from cell division.

Xenopus myc proto-oncogene during development: expression as a stable maternal mRNA uncoupled from cell division.

Metabolism of Poly (A) Sequences of Maternal mRNA during Progesterone-Induced Maturation of Xenopus laevis Oocytes

Transcription terminator-like element within a Saccharomyces cerevisiae promoter region.

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