Differential acquisition of human antibody responses to Plasmodium falciparum according to intensity of exposure to Anopheles bites

Sarr, Joseph; Samb, Badara; Sagna, Aloïse; Fortin, Sonia; Doucouré, Souleymane; Sow, Cheikh; Senghor, Simon; Gaayeb, Lobna; Guindo, S.; Schacht, Anne‐Marie; Rogerie, F; Hermann, Emmanuel; Dia, Ibrahima; Konaté, Lassana; Riveau, Gilles; Remoué, Franck

doi:10.1016/j.trstmh.2012.05.006

Cited by 14 publications

(24 citation statements)

References 33 publications

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“…Only a few epidemiological studies have previously reported an association between exposure to Anopheles immunomodulatory saliva and acquisition of natural immunity to Plasmodium in natural settings. Sarr et al reported that children with higher exposure to Anopheles bites presented a down-regulated IgG1 response to whole Plasmodium extract and to CSP Ag compared to children lower exposed, whereas no effect was observed for the IgG3 isotype response [34]. Dechavanne et al reported that an environmental variable (quantitative index related to the spatiotemporal risk of exposure to Anopheles mosquitoes) was significantly associated with high anti-Plasmodium Ab levels in infants (6-18 month old infants) [13].…”

Section: Discussionmentioning

confidence: 99%

“…Sarr et al showed a modulation of the balance of cytophilic Ab responses to parasite Ags according to the level of exposure of children to Anopheles bites. High exposure to Anopheles bites seemed to down-regulate the protective IgG1 Ab responses to whole Plasmodium extracts and to CSP Ag, whereas specific IgG3 responses were similar for the two Plasmodium Ags in villages exposed to either low or high levels of Anopheles bites [34].…”

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confidence: 89%

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Pattern of antibody responses to Plasmodium falciparum antigens in individuals differentially exposed to Anopheles bites

Aka

Traoré

Sagna

et al. 2020

Malar J

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Background: In malaria-endemic areas, human populations are frequently exposed to immunomodulatory salivary components injected during mosquito blood feeding. The consequences on pathogen-specific immune responses are not well known. This study evaluated and compared the humoral responses specific to merozoite stage vaccine candidates of Plasmodium falciparum, in children differentially exposed to Anopheles bites in a natural setting. Methods:The cross-sectional study was carried out in Bouaké (Côte d'Ivoire) where entomological data and blood samples from children (0-14 years) were collected in two sites with similar malaria prevalence. Antibody (IgG, IgG1, IgG3) responses to PfAMA1 and PfMSP1 were evaluated by ELISA. Univariate and multivariate analysis were performed to assess the relationship between the immune responses to P. falciparum antigens and exposure to Anopheles bites in the total cohort and in each site, separately. The individual level of exposure to Anopheles bites was evaluated by quantifying specific IgG response to the Anopheles gSG6-P1 salivary peptide, which represents a proxy of Anopheles exposure.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 89%

Pattern of antibody responses to Plasmodium falciparum antigens in individuals differentially exposed to Anopheles bites

Aka

Traoré

Sagna

et al. 2020

Malar J

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“…47 These differences may be a result of dissimilarities in exposure, age, and time of residence of the studied populations in the endemic area because several studies have demonstrated a direct effect of these parameters on the antibody response to several plasmodial antigens. 18,19,45,48 Given that the repertoire of the host erythrocyte receptors available in specific populations will select for the expression of specific parasite ligands that can mediate a successful erythrocyte invasion, the immune response to such ligands may vary in different populations, correlating to their expression. We can also hypothesize that the difference in immunoreactivity may be due the differential usage of the EBA-175-glycophorin A invasion pathway.…”

Section: Discussionmentioning

confidence: 99%

Synthetic Antigens Derived from Plasmodium falciparum Sporozoite, Liver, and Blood Stages: Naturally Acquired Immune Response and Human Leukocyte Antigen Associations in Individuals Living in a Brazilian Endemic Area

Pratt-Riccio

Perce-da-Silva

Lima-Junior

et al. 2017

The American Journal of Tropical Medicine and Hygiene

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Peptide vaccine strategies using -derived antigens have emerged as an attractive approach against malaria. However, relatively few studies have been conducted with malaria-exposed populations from non-African countries. Herein, the seroepidemiological profile against of naturally exposed individuals from a Brazilian malaria-endemic area against synthetic peptides derived from vaccine candidates circumsporozoite protein (CSP), liver stage antigen-1 (LSA-1), erythrocyte binding antigen-175 (EBA-175), and merozoite surface protein-3 (MSP-3) was investigated. Moreover, human leukocyte antigen (HLA)-DRB1* and HLA-DQB1* were evaluated to characterize genetic modulation of humoral responsiveness to these antigens. The study was performed using blood samples from 187 individuals living in rural malaria-endemic villages situated near Porto Velho, Rondônia State. Specific IgG and IgM antibodies and IgG subclasses were detected by enzyme-linked immunosorbent assay, and HLA-DRB1* and HLA-DQB1* low-resolution typing was performed by PCR-SSP. All four synthetic peptides were broadly recognized by naturally acquired antibodies. Regarding the IgG subclass profile, only CSP induced IgG1 and IgG3 antibodies, which is an important fact given that the acquisition of protective immunity appears to be associated with the cytophilicity of IgG1 and IgG3 antibodies. HLA-DRB1*11 and HLA-DQB1*7 had the lowest odds of responding to EBA-175. Our results showed that CSP, LSA-1, EBA, and MSP-3 are immunogenic in natural conditions of exposure and that anti-EBA antibody responses appear to be modulated by HLA class II antigens.

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“…Such bias is somewhat unexpected, as IgG3 antibodies have a shorter half-life than other subclasses. It has been suggested that repeated parasite exposure may sustain high-levels of IgG3 antibodies (Kinyanjui et al, 2003; Sarr et al, 2012), while recent evidence reveals a genetic contribution to differential subclass production against malaria (Afridi et al, 2012). It is also possible the higher sensitivity of commercial anti-IgG3 detection antibodies may have led to an apparent IgG3 bias.…”

Section: Discussionmentioning

confidence: 99%

Effect of transmission intensity and age on subclass antibody responses to Plasmodium falciparum pre-erythrocytic and blood-stage antigens

et al. 2015

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Cytophilic immunoglobulin (IgG) subclass responses (IgG1 and IgG3) to Plasmodium falciparum antigens have been associated with protection from malaria, yet the relative importance of transmission intensity and age in generation of subclass responses to pre-erythrocytic and blood-stage antigens have not been clearly defined. We analyzed IgG subclass responses to the pre-erythrocytic antigens CSP, LSA-1, and TRAP and the blood-stage antigens AMA-1, EBA-175, and MSP-1 in asymptomatic residents age 2 years or older in stable (n=116) and unstable (n=96) transmission areas in Western Kenya. In the area of stable malaria transmission, a high prevalence of cytophilic (IgG1 and IgG3) antibodies to each antigen was seen in all age groups. Prevalence and levels of cytophilic antibodies to pre-erythrocytic and blood-stage P. falciparum antigens increased with age in the unstable transmission area, yet IgG1 and IgG3 responses to most antigens for all ages in the unstable transmission area were less prevalent and lower in magnitude than even the youngest age group from the stable transmission area. The dominance of cytophilic responses over non-cytophilic (IgG2 and IgG4) was more pronounced in the stable transmission area, and the ratio of IgG3 over IgG1 generally increased with age. In the unstable transmission area, the ratio of cytophilic to non-cytophilic antibodies did not increase with age, and tended to be IgG3-biased for pre-erythrocytic antigens yet IgG1-biased for blood-stage antigens. The differences between areas could not be attributed to active parasitemia status, as there were minimal differences in antibody responses between those positive and negative for Plasmodium infection by microscopy in the stable transmission area. Individuals in areas of unstable transmission have low cytophilic to non-cytophilic IgG subclass ratios and low IgG3:IgG1 ratios to P. falciparum antigens. These imbalances could contribute to the persistent risk of clinical malaria in these areas and serve as population-level, age-specific biomarkers of transmission.

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Differential acquisition of human antibody responses to Plasmodium falciparum according to intensity of exposure to Anopheles bites

Cited by 14 publications

References 33 publications

Pattern of antibody responses to Plasmodium falciparum antigens in individuals differentially exposed to Anopheles bites

Pattern of antibody responses to Plasmodium falciparum antigens in individuals differentially exposed to Anopheles bites

Synthetic Antigens Derived from Plasmodium falciparum Sporozoite, Liver, and Blood Stages: Naturally Acquired Immune Response and Human Leukocyte Antigen Associations in Individuals Living in a Brazilian Endemic Area

Effect of transmission intensity and age on subclass antibody responses to Plasmodium falciparum pre-erythrocytic and blood-stage antigens

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