Differential Activation of Smads in HeLa and SiHa Cells That Differ in Their Response to Transforming Growth Factor-β

Maliekal, Tessy Thomas; Anto, Ruby John; Karunagaran, Devarajan

doi:10.1074/jbc.m404568200

Cited by 23 publications

(19 citation statements)

References 46 publications

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“…Using SMAD2/SMAD3 knock out fibroblast cell-lines, SMAD 2 and 3 independent actions of TGF-β have been reported earlier [36]. In addition, SMAD independent signalling by TGF-β mediated by other pathways like the MAP kinases namely the p38, ERK and the JNK pathways is also reported [18-20,37-40]. Activation of the p38 and the JNK pathways by TGF-β involves a TGF-β activated kinase called TAK1 (reviewed in [41,42]).…”

Section: Discussionmentioning

confidence: 83%

Expression profiling of genes regulated by TGF-beta: Differential regulation in normal and tumour cells

et al. 2007

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Background: TGF-beta is one of the key cytokines implicated in various disease processes including cancer. TGF-beta inhibits growth and promotes apoptosis in normal epithelial cells and in contrast, acts as a pro-tumour cytokine by promoting tumour angiogenesis, immune-escape and metastasis. It is not clear if various actions of TGF-beta on normal and tumour cells are due to differential gene regulations. Hence we studied the regulation of gene expression by TGF-beta in normal and cancer cells.

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Section: Discussionmentioning

confidence: 83%

Expression profiling of genes regulated by TGF-beta: Differential regulation in normal and tumour cells

et al. 2007

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“…To analyze whether this binding is related to Smad-binding activity, we tested a consensus Smad-binding element sequence in competition assays. 34 This sequence efficiently inhibited binding of the labeled probe to nuclear proteins, which strongly suggested that Smads are required nuclear proteins in the extract for binding to the 29-bp Cox2 element. Stimulation of MECs with BMP6 for 4 hours retarded the mobility and enhanced the intensity of the DNAprotein interaction (Figure 5D), suggesting that BMP6 remodels or posttranslationally modifies the protein complex that binds to this element.…”

Section: Transcriptional Activation Of the Cox2 Promoter By Bmp6mentioning

confidence: 99%

Gene expression profiles identify a role for cyclooxygenase 2–dependent prostanoid generation in BMP6-induced angiogenic responses

Ren

Charles

Zhang

et al. 2006

Blood

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The bone morphogenetic protein (BMP) family of proteins participates in regulation of angiogenesis in physiologic and pathologic conditions. To investigate the molecular mechanisms that contribute to BMP-dependent angiogenic signaling, we performed gene expression profiling of BMP6-treated mouse endothelial cells. We detected 77 mRNAs that were differentially regulated after BMP6 stimulation. Of these, cyclooxygenase 2 (Cox2) was among the most highly up-regulated by BMP stimulation, suggesting a role for

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“…Western blotting was performed as described before27. Briefly, cell lysates were prepared in RIPA lysis buffer and the bands were resolved by 10% SDS-PAGE, and transferred to nitrocellulose membrane for immunoblot analysis.…”

Section: Methodsmentioning

confidence: 99%

TM1-IR680 peptide for assessment of surgical margin and lymph node metastasis in murine orthotopic model of oral cancer

Kochurani

Nair

et al. 2016

Sci Rep

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Treatment outcome after surgical removal in oral carcinoma is poor due to inadequate methodologies available for marking surgical margins. Even though some methodologies for intraoperative margin assessment are under clinical and preclinical trials for other solid tumours, a promising modality for oral cancer surgery is not developed. Fluorescent-based optical imaging using Near Infrared (NIR) dyes tagged to tumour specific target will be an optimal tool for this purpose. One such target, Gastrin Releasing Peptide Receptor (GRPR) was selected for the study, and its binding peptide, TM1-IR680, was tested for its efficacy for surgical margin prediction in murine orthotopic model of oral cancer, derived from primary samples. Here, for the first time in a preclinical analysis, we show that the size and margin of oral cancer can be predicted, as revealed by 3D-imaging. Interestingly, the peptide was sensitive enough to detect lymph nodes that harboured dispersed tumour cells before colonization, which was impossible to identify by conventional histopathology. We recommend the use of TM1-NIR dyes alone or in combination with other technologies to improve the clinical outcome of oral cancer surgery.

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Differential Activation of Smads in HeLa and SiHa Cells That Differ in Their Response to Transforming Growth Factor-β

Abstract: We assessed the responsiveness of six human cervical cancer cell lines to transforming growth factor (TGF) -

Cited by 23 publications

References 46 publications

Expression profiling of genes regulated by TGF-beta: Differential regulation in normal and tumour cells

Expression profiling of genes regulated by TGF-beta: Differential regulation in normal and tumour cells

Gene expression profiles identify a role for cyclooxygenase 2–dependent prostanoid generation in BMP6-induced angiogenic responses

TM1-IR680 peptide for assessment of surgical margin and lymph node metastasis in murine orthotopic model of oral cancer

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