DIFFERENTIAL AGGLUTINATION OF PARTICULATE Vi AND O ANTIGENS BY THE IgM AND IgG CLASS ANTIBODIES

Chau, Pui Hing; Mh, Ng

doi:10.1038/icb.1978.5

Aust J Exp Biol Med

1978

DOI: 10.1038/icb.1978.5

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DIFFERENTIAL AGGLUTINATION OF PARTICULATE Vi AND O ANTIGENS BY THE IgM AND IgG CLASS ANTIBODIES

Pui Hing Chau

Ng Mh

Abstract: INTRODUCTION.

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1981

1985

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“…On the other hand, agglutinins against S. typhi are often obtained in response to nontyphoid Salmonella infections (21,24,25) and sometimes in diseases not attributable to Salmonella (20). The most important shortcoming, however, as the test is usually performed in the clinical laboratories, is that it is primarily a test of immunoglobulin M (IgM) antibodies (5,6,13). Hemagglutination tests, which offer several advantages over bacterial agglutinations in terms of sensitivity and versatility (19), often depend on IgM antibodies (2,31).…”

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confidence: 99%

Improved Serodiagnosis of Salmonella Enteric Fevers by an Enzyme-Linked Immunosorbent Assay

1981

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The value of the enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of typhoid or paratyphoid fevers was tested with a collection of five sets of sera from 23 individuals who had no history of Salmonella infection from a nonendemic area (set 1) and from 143 patients from a highly endemic area (sets 2 to 5). The test was performed with a crude cell envelope fraction derived from Salmonella typhi. On the basis of results with sera in set 1, titers >160 (inverse of serum dilution) for immunoglobulin M (IgM) or >500 for IgG were regarded as signifying a specific antibody response. These titers were occasionally exceeded in sera in set 2, derived from patients with no clinical or laboratory evidence of Salmonella infection. In presumptive cases of typhoid or paratyphoid fevers (culture positive and agglutination negative or vice versa; 13 patients, set 3, and 26 patients, set 4, respectively), IgG antibodies were encountered more frequently than IgM, but some sera were negative in each group. In 81 confirmed cases (culture and agglutination positive, set 5) IgG antibodies were detected in at least one of the paired sera from all patients, and the IgG titers were higher (median 3,500) than those of the IgM antibodies (median 600). There was no evidence of a significant difference in IgM or IgG titers between acute- and convalescent-phase sera. The titers of 10 paired sera from patients from whom S. enteritidis , bioserotype paratyphi A, was isolated were somewhat lower than those of 84 patients with S. typhi isolation (serogroup D). There was some correlation between O antigen agglutination and IgM enzyme-linked immunosorbent assay, but otherwise the titers in the two serological tests varied independently. Of 11 patients in set 3 (2 patients with serogroup B agglutinins omitted), 1 belonged to blood group O, in contrast to 54 group O among 107 agglutination-positive patients, a distribution not necessarily reflected by the enzyme-linked immuno-sorbent assay. This study has provided good evidence that the enzyme-linked immunosorbent assay can serve as a valuable aid to the diagnosis of Salmonella infection in an endemic area, as a substitute for the agglutination test.

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Improved Serodiagnosis of Salmonella Enteric Fevers by an Enzyme-Linked Immunosorbent Assay

1981

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Antibody Response to Somatic Antigen ofSalmonella typhiin Areas Endemic and Non-Endemic for Typhoid Fever

et al. 1985

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In sera obtained between the 6th and the 30th day from 16 Austrian and 26 Hong Kong patients with culturally verified typhoid fever, agglutinating antibodies (microagglutination test) at significant titers were detected in 93% of the Austrian (median titer: 640) but in only 50% of the Hong Kong patients (median titer: 240). Similar results (93% and 54% positive sera respectively) were obtained for specific IgM as assessed by the enzyme-linked immunosorbent assay (ELISA) using lipopolysaccharide of S. typhi as antigen (median relative titer: 0.32 and 0.21 respectively). In contrast, specific IgG at significant concentrations were found in only 69% of the Austrian (median relative titer: 0.16) but 88% of the Hong Kong sera (median relative titer: 0.71). The (IgM-detecting) microagglutination test may be sufficiently diagnostic for typhoid fever in nonendemic areas such as Austria. In endemic regions like Hong Kong, however, tests indicative for early specific IgG are indispensable for serological diagnosis of the disease. The ELISA proved useful and is an example for such tests.

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DIFFERENTIAL AGGLUTINATION OF PARTICULATE Vi AND O ANTIGENS BY THE IgM AND IgG CLASS ANTIBODIES

Abstract: INTRODUCTION.

Cited by 2 publications

References 5 publications

Improved Serodiagnosis of Salmonella Enteric Fevers by an Enzyme-Linked Immunosorbent Assay

Improved Serodiagnosis of Salmonella Enteric Fevers by an Enzyme-Linked Immunosorbent Assay

Antibody Response to Somatic Antigen ofSalmonella typhiin Areas Endemic and Non-Endemic for Typhoid Fever

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